Recent Advances in the CRISPR/Cas-Based Nucleic Acid Biosensor for Food Analysis: A Review.

Food safety is a major public health issue of global concern. In recent years, the CRISPR/Cas system has shown promise in the field of molecular detection. The system has been coupled with various nucleic acid amplification methods and combined with different signal output systems to develop a new generation of CRISPR/Cas-based nucleic acid biosensor technology.

MGACL: Prediction Drug-Protein Interaction Based on Meta-Graph Association-Aware Contrastive Learning.

The identification of drug-target interaction (DTI) is crucial for drug discovery. However, how to reduce the graph neural network's false positives due to its bias and negative transfer in the original bipartite graph remains to be clarified. Considering that the impact of heterogeneous auxiliary information on DTI varies depending on the drug and target, we established an adaptive enhanced personalized meta-knowledge transfer network named eta raph ssociation-Aware ontrastive earning (MGACL), which can transfer personalized heterogeneous auxiliary information from different nodes and reduce data bias.

Leveraging mitochondrial-programmed cell death dynamics to enhance prognostic accuracy and immunotherapy efficacy in lung adenocarcinoma.

Background: Lung adenocarcinoma (LUAD) is a highly heterogeneous disease, posing significant challenges to accurate prognosis prediction. Mitochondria play a central role in the energy metabolism of eukaryotic cells and can influence programmed cell death (PCD) mechanisms, which are critical in tumorigenesis and cancer progression. However, the prognostic significance of the interplay between mitochondrial function and PCD in LUAD requires further investigation.

Split G-Quadruplex Programmed Recyclable AIE-Biosensor for Label-Free Detection of miRNA in Acute Kidney Injury.

We herein rationally designed a target-recyclable AIE-biosensor based on a split G-quadruplex for label-free detection of miRNA in acute kidney injury. Initially, the PG was in an "OFF" state, and the two split segments (G4-a and G4-b) of G4 were tethered at the two terminals of P1 and far away from each other due to the rigid duplex structure formed by the partially complementary intermediate sequences of P2 and P1, bringing MG with quenched fluorescence. In the presence of target, the 5'-PO P2 was displaced from PG probe and competitively hybridized with target, which led to G4-a and G4-b tending to form an intact intermolecular G-quadruplex, providing sites for MG intercalation, thus generating an activated "ON" fluorescence signal due to the restriction of intermolecular motion.

Efficient Uranium Removal from Aqueous Solutions Using Silica-Based Adsorbents Functionalized with Various Polyamines.

With the rapid development of nuclear energy, the contamination of environmental water systems by uranium has become a significant threat to human health. To efficiently remove uranium from these systems, three types of silica-based polyamine resins-SiPMA-DETA (SiPMA: silica/poly methyl acrylate; DETA: diethylenetriamine), SiPMA-TETA (TETA: triethylenetetramine), and SiPMA-TEPA (TEPA: tetraethylenepentamine)-were successfully prepared, characterized, and evaluated in batch experiments. Characterization results showed that the silica-based polyamine resins were successfully prepared, and they exhibited a uniform shape and high specific surface area.

Chromosome-Scale Genome and Transcriptomic Analyses Reveal Differential Regulation of Terpenoid Secondary Metabolites in .

Construction of the genome of , a species of edible mushroom, and identification of the genes involved in terpenoid biosynthesis can determine the biology and genetics of terpenoids. The present study describes the assembly of a high-quality chromosome-scale genome of using Pacbio HiFi sequencing and Hi-C technology. This genome consisted of 13 chromosomes, a total size of 43.

Mediating effects of physical activities and cognitive function on the relationship between dietary diversity and all-cause mortality in community-dwelling older adults.

Background: Although dietary diversity (DD) has been confirmed to be associated with multiple health outcomes and longevity in older people, the related mechanisms have not been elucidated. In this study, we explored the mediating roles of physical activities and cognitive function in the relationship between DD and all-cause mortality.

Methods: We recruited 34 068 community-dwelling older adults aged ≥60 years from the Chinese Longitudinal Healthy Longevity Study and followed them up until 2018.

Global burden, trends and inequalities for typhoid and paratyphoid fever among children younger than 15 years over the past 30 years.

Background: Typhoid and paratyphoid fever, collectively known as enteric fever, are systemic infections caused by Salmonella enterica and are highly prevalent in children. We aimed to describe the global burden, trends and inequalities of enteric fever among children under 15 years from 1990 to 2019 based on data from the Global Burden of Diseases, Injuries, and Risk Factors Study 2019 (GBD 2019).

Methods: In this secondary analysis of GBD 2019, we extracted data for incident cases, deaths and disability-adjusted life-years (DALYs) as measures of enteric fever burden.

Diacylglycerol O-acyltransferase 2, a Novel Target of Flavivirus NS2B3 Protease, Promotes Zika Virus Replication by Regulating Lipid Droplet Formation.

Various lipid metabolism-related factors are essential for Zika virus (ZIKV) replication. In this study, we revealed a crucial role of diacylglycerol O-acyltransferase 2 (DGAT2) in ZIKV replication using a short hairpin RNA-based gene knockdown technique. The replication of ZIKV was significantly inhibited by DGAT2 depletion in multiple cell lines and restored by trans-complementation with DGAT2.

The recent research progress in the application of the nanozyme-hydrogel composite system for drug delivery.

Hydrogels, comprising 3D hydrophilic polymer networks, have emerged as promising biomaterial candidates for emulating the structure of biological tissues and delivering drugs through topical administration with good biocompatibility. Nanozymes can catalyze endogenous biomolecules, thereby initiating or inhibiting biological processes. A nanozyme-hydrogel composite inherits the biological functions of hydrogels and nanozymes, where the nanozyme serves as the catalytic core and the hydrogel forms the structural scaffold.

PVDF nanocomposites with aligned boron nitride nanosheets and dispersed BaTiO@PEG nanoparticles by a superspreading strategy towards high energy density.

The limited improvement in discharge energy density () of polyvinylidene fluoride (PVDF) nanocomposites filled with low-dielectric-constant () nanosheets has been addressed by using a superspreading layering strategy. The integration of highly aligned boron nitride nanosheets (BNNS) and well-dispersed BaTiO@PEG (BT@PEG) nanoparticles into the PVDF matrix results in a significant enhancement, increasing and by 52% and 64%, respectively, and achieving an of up to 20.12 J cm.

pH-Responsive MOF Nanoparticles Equipped with Hydrophilic "Armor" Assist Fungicides in Controlling Peanut Southern Blight.

The development of novel, safe, and efficient pest and disease control technologies for agricultural crops remains a pivotal area of research. In this study, by combining ZIF-8 and ZIF-90, a water-stable, pH-responsive bilayer MOF nanoparticle (NP) named Z8@Z90 was created, and tebuconazole (TEB) was added to form T@Z8@Z90, used for controlling peanut southern blight. The loading efficiency of TEB within the T@Z8@Z90 reached 26.

Controllable Regulation of CO Adsorption Behavior via Precise Charge Donation Modulation for Highly Selective CO Electroreduction to Formic Acid.

The synthesis of value-added products via CO electroreduction (COER) is of great significance, but the development of efficient and versatile strategies for the controllable selectivity tuning is extremely challenging. Herein, the tuning of COER selectivity through the modulation of CO adsorption behavior is proposed. Using the constructed zeolitic MOF (SNNU-339), CO adsorption behavior is controllably changed from *CO to CO* via the precise ligand-to-metal charge donation (LTMCD) regulation.

Short-term ozone exposure on stroke mortality and mitigation by greenness in rural and urban areas of Shandong Province, China.

Background: Short-term exposure to ozone (O) has been associated with higher stroke mortality, but it is unclear whether this association differs between urban and rural areas. The study aimed to compare the association between short-term exposure to O and ischaemic and haemorrhagic stroke mortality across rural and urban areas and further investigate the potential impacts of modifiers, such as greenness, on this association.

Methods: A multi-county time-series analysis was carried out in 19 counties of Shandong Province from 2013 to 2019.

Innovative management strategies for addressing paediatric medical staff shortages in underdeveloped cities in developing countries.

Background: Paediatric professional scarcity and uneven distribution is acute in underdeveloped regions, exacerbated by COVID-19's workload surge and burnout, highlighting the need for strengthened prevention and response measures.

Aim: Propose an effective talent management model to address the challenge of paediatric medical personnel shortage and lack of management experience in Underdeveloped cities of developing countries.

Methods: A crisis management plan has been implemented in a paediatric hospital in Henan, China, with a talent framework to ensure a skilled, stable workforce.

Eutrophication promotes resource use efficiency and toxin production of Microcystis in a future climate warming scenario.

Addressing the risks of cyanobacterial blooms and toxin production under ongoing and accelerating eutrophication and climate warming is crucial for both water ecosystem services and human health. Therefore, we here explored the interactive effects of eutrophication and warming on freshwater ecosystems, focusing on Microcystis and its cyanotoxin production. We employed a large-scale mesocosm system simulating future climate warming scenarios in concert with varying degrees of nutrient enrichment.

RAdiotherapy Dose Accumulation Routine (RADAR)-A Novel Dose Accumulation Script With Built-In Uncertainty.

Purpose: To incorporate uncertainty into dose accumulation for reirradiation.

Methods And Materials: The RAdiotherapy Dose Accumulation Routine (RADAR) script for the Eclipse treatment planning system (Varian Medical Systems) is described, and the voxel-wise ellipsoid search algorithm is introduced as a means of incorporating uncertainty. RADAR is first demonstrated on a test patient reirradiated to the spine, illustrating the effect of the uncertainty algorithm.

Adaptive multi-epitope targeting and avidity-enhanced nanobody platform for ultrapotent, durable antiviral therapy.

Pathogens constantly evolve and can develop mutations that evade host immunity and treatment. Addressing these escape mechanisms requires targeting evolutionarily conserved vulnerabilities, as mutations in these regions often impose fitness costs. We introduce adaptive multi-epitope targeting with enhanced avidity (AMETA), a modular and multivalent nanobody platform that conjugates potent bispecific nanobodies to a human immunoglobulin M (IgM) scaffold.

Metal-Organic Framework-Derived Ni-Doped Indium Oxide Nanorods for Parts per Billion-Level Nitrogen Dioxide Gas Sensing at High Humidity.

Detecting parts per billion (ppb)-level nitrogen dioxide in high-moisture environments at room temperature without reducing sensing performance is a well-recognized significant challenge for metal oxide-based gas sensors. In this study, metal-organic framework-derived nickel-doped indium oxide (Ni-doped InO) mesoporous nanorods were prepared by a solvothermal method combined with the calcination process. The sensors prepared using the obtained Ni-doped InO nanorods showcase an ultrahigh response, low detection limit, and excellent selectivity.

Consensus on the prevention and repair of titanium mesh exposed wound after cranioplasty (2024 edition).

Titanium mesh exposure after cranioplasty is the most serious complication of this procedure. Although some clinical experience has been gradually accumulated over the years in the diagnosis and treatment of titanium mesh exposure, the treatment is often not standardized and it is difficult to achieve satisfactory repair results due to insufficient understanding of its pathogenesis and concurrent infections. To normalize the diagnosis and treatment of titanium mesh exposed wounds after cranioplasty and improve the therapeutic effect and the quality of life of patients, the Wound Repair Professional Committee of Chinese Medical Doctor Association organized an expert discussion based on the literature and current diagnosis and treatment status of titanium mesh exposed wounds after cranioplasty at home and abroad, and reached a consensus on the pathogenesis, preventive measures, and diagnosis and treatment strategies of titanium mesh exposed wounds after cranioplasty to provide reference for relevant clinicians.