Knockdown of regulator of Calcineurin 2 promotes transcription factor EB-mediated lipophagy to prevent non-alcoholic fatty liver disease.

Non-alcoholic fatty liver disease (NAFLD) is a major cause of chronic liver disease. The present work aimed to explore the function of regulator of Calcineurin 2 (RCAN2) in NAFLD and its related mechanisms. Mice were fed with high-fat diet (HFD) to construct NAFLD model.

Dynamic effects of fixed orthodontic treatment on oral health and oral microbiota: a prospective study.

Objective: This prospective clinical study examined the effects of fixed orthodontic appliances on oral hygiene and assessed changes in the oral microflora structure of orthodontic patients using high-throughput sequencing technology.

Methods: A total of 45 patients undergoing initial fixed orthodontic treatment were recruited from three hospitals in Beijing, China. Clinical parameters and oral hygiene habits questionnaire were recorded at pre-treatment (T0) and at a 6-month follow-up (T1).

Critical roles of extracellular vesicles in periodontal disease and regeneration.

Extracellular vesicles (EVs) are evolutionarily conserved communication mediators that play key roles in the development of periodontal disease as well as in regeneration processes. This concise review first outlines the pathogenic mechanisms through which EVs derived from bacteria lead to the progression of periodontitis, with a focus on the enrichment of virulence factors, the amplification of immune responses, and the induction of bone destruction as key aspects influenced by bacterial EVs. This review aims to elucidate the positive effects of EVs derived from mesenchymal stem cells (MSC-EVs) on periodontal tissue regeneration.

Development and validation of a psychotherapy change motivation scale.

Objective: Develop a Psychotherapy Change Motivation Scale (PCMS) with robust psychometric properties.

Method: A literature review, semi-structured interviews, and expert evaluation were used to draft the scale. The scale was administered to participants receiving psychological counseling or therapy at specialized hospital psychological intervention centers, university mental health education centers, and social psychological counseling agencies using convenience sampling.

Effect of Baduanjin exercise on health and functional status in patients with chronic obstructive pulmonary disease: a community-based, cluster-randomized controlled trial.

Baduanjin has been shown to be an effective method of exercise for promoting body function in patients with chronic obstructive pulmonary disease (COPD). However, this method is rarely used by rural doctors in rural communities. The aim of this study was to compare the therapeutic effects of Baduanjin exercise with those of conventional pulmonary rehabilitation (CPR) implemented by rural doctors in patients with COPD.

Tough, antibacterial, antioxidant, antifogging and washable chitosan/nanocellulose-based edible coatings for grape preservation.

This study focused on extracting nanocellulose from food processing waste to create a multi-functional edible coating for preserving grapes. Nanocellulose, in the form of short rods with diameters ranging from 30 to 130 nm, was extracted from soy hulls. Edible coatings were then prepared through an ion cross-linking method.

mutation-related immune checkpoint molecule absent in melanoma 2 () contributes to immune infiltration in pediatric and adult acute myeloid leukemia: evidence from bioinformatics analysis.

Background: The use of FMS-like tyrosine kinase 3 () as a crucial target for kinase inhibitors is well established, but its association with immune infiltration remains unclear. This study aimed to explore the relationship between mutations and immune checkpoint molecules (ICMs) in patients with acute myeloid leukemia (AML).

Methods: The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) databases were used to identify the ICMs associated with mutations.

The F204S mutation in adrenodoxin oxidoreductase drives salinomycin resistance in Eimeria tenella.

Salinomycin is a polyether ionophore widely used for the treatment of coccidiosis in poultry. However, the emergence of coccidia strains resistant to salinomycin presents challenges for control efforts, and the mechanisms underlying this resistance in Eimeria remain inadequately understood. In this study, 78 stable salinomycin-resistant strains were generated through experimental evolution approaches.

A STT3A-dependent PD-L1 glycosylation modification mediated by GMPS drives tumor immune evasion in hepatocellular carcinoma.

Hepatocellular carcinoma (HCC) is a malignant tumor characterized by rapid progression. To explore the regulatory mechanism of rapid tumor growth and metastasis, we conducted proteomic and scRNA-Seq analyses on advanced HCC tissues and identified a significant molecule, guanine monophosphate synthase (GMPS), closely associated with the immune evasion in HCC. We analyzed the immune microenvironment characteristics remodeled by GMPS using scRNA-Seq and found GMPS induced tumor immune evasion in HCC by impairing the tumor-killing function of CD8  T cells.

StUBC13, a Ubiquitin-Conjugating Enzyme, Positively Regulates Salt and Osmotic Stresses in Potato.

Protein ubiquitination is an important regulatory mechanism for biological growth and development against environmental influences, and can affect several biological processes, including the growth, development, and stress responses of plants. However, the function of potato-related ubiquitin-conjugating enzymes in abiotic stress tolerance is poorly understood. In this study, a with a UBC conserved structural domain was identified in potato and its function was investigated under osmotic stress and salt stress conditions.

Effect of graded exercise rehabilitation based on pulmonary function classification on dyspnea, pulmonary function, and exercise capacity in elderly lung cancer patients.

Objective: To investigate the effects of graded exercise rehabilitation training tailored to pulmonary function classification on dyspnea, pulmonary function, and exercise capacity during postoperative rehabilitation in elderly patients following lung cancer surgery.

Methods: A retrospective analysis was conducted on clinical data from 168 elderly patients undergoing postoperative rehabilitation after lung cancer surgery at Panjin Liaohe Oilfield Gem Flower Hospital from January 2021 to December 2022. Patients were divided into two groups based on the rehabilitation received: the control group (n=71), receiving standard rehabilitation, and the study group (n=97), receiving additional graded exercise rehabilitation based on pulmonary function classification.

Development and validation of a risk score model for patient stratification and personalized management of papillary thyroid cancer.

Background: The status of central lymph node (CLN) is a crucial determinant for the initial treatment of papillary thyroid cancer (PTC), but preoperative ultrasound (US) has limited ability to accurately assess their condition. This study aimed to develop a risk score model for risk stratification of CLN metastasis in unifocal PTC patients to guide the initial treatment.

Methods: A total of 5,374 patients diagnosed with unifocal PTC at Union Hospital between November 2009 and August 2022 were finally enrolled in the analysis, including 3,542 patients in derivation cohort and 1,832 patients in validation cohort.

Temperature-dependent fold-switching mechanism of the circadian clock protein KaiB.

The oscillator of the cyanobacterial circadian clock relies on the ability of the KaiB protein to switch reversibly between a stable ground-state fold (gsKaiB) and an unstable fold-switched fold (fsKaiB). Rare fold-switching events by KaiB provide a critical delay in the negative feedback loop of this posttranslational oscillator. In this study, we experimentally and computationally investigate the temperature dependence of fold switching and its mechanism.

A cross-tissue transcriptome-wide association study identifies WDPCP as a potential susceptibility gene for coronary atherosclerosis.

Background: Coronary atherosclerosis (CAS) is a complex chronic inflammatory disease with significant genetic and environmental contributions. While genome-wide association studies (GWAS) have pinpointed many risk loci, over 75 % are in non-coding regions, complicating functional analysis and understanding gene-disease mechanisms.

Methods: We conducted a cross-tissue transcriptome-wide association study (TWAS) using data from the GWAS Catalog (16,041 cases, 440,307 controls) and the Genotype-Tissue Expression (GTEx) v8 eQTL dataset.

Erratum: MT1G is Silenced by DNA Methylation and Contributes to the Pathogenesis of Hepatocellular Carcinoma: Erratum.

[This corrects the article DOI: 10.7150/jca.25680.

Harnessing Nanotechnology for Gout Therapy: Colchicine-Loaded Nanoparticles Regulate Macrophage Polarization and Reduce Inflammation.

Gout is a disease caused by hyperuricemia, characterized by inflammation reactions triggered by macrophage polarization. Colchicine is a commonly used drug for gout treatment, but its mechanism of action remains unclear. The aim of this study was to investigate the regulatory effect of colchicine on macrophage polarization to enhance the therapeutic effectiveness against gout inflammation.

Determinants of the low use of Thailand's Universal Coverage Scheme: a national cross-sectional study.

Introduction: Thailand's Universal Coverage Scheme (UCS) has increased overall healthcare use by offering free healthcare for about 76% of the entire population since it was introduced in 2002. However, a considerable number of beneficiaries have continued to depend on private healthcare, and the low use of the UCS has been cited as a challenge to accomplishing the policy's goal of universal access to healthcare. Thus, this study divided healthcare use into three patterns (self-medication, private providers, and UCS) and investigated the socio-demographic characteristics of non-users of the UCS and their reasons for non-use.

Temporal Incision Super-high Superficial Musculoaponeurotic System Face Lift for Chinese Patients.

Background: This investigation assessed patient satisfaction with a temporal incision technique using a super-high superficial musculoaponeurotic system (SMAS) flap for midface lifting in a Chinese patient cohort.

Methods: From July 2019 to July 2023, 95 patients underwent midface lifts via a temporal incision approach at our institution. The extent of SMAS flap dissection spanned 2 cm above the outer canthus, to the lower margin of the zygomatic arch inferiorly, and medially to the zygomaticus major muscle.

In Situ Grown LiTe Enhanced Lithium Metal Anode Interfacial Kinetics.

Lithium metal anode (LMA) is expected to be the ideal anode material for future high-energy-density batteries, but regulating the complex electrolyte-anode interface remains a challenge. In this work, a stable LiTe coating is formed on the surface of commercial copper mesh (LTCM) using a simple and quick method to improve lithium metal anode interfacial kinetics. LiTe possesses a strong affinity for both Li and TFSI anions, which reduces the lithium nucleation barrier and guides the formation of inorganic-rich SEI, accelerates the diffusion of Li, and promotes the growth of lithium metal along the plane.

Corrigendum to 'Comparing sedation protocols for endoscopic retrograde cholangiopancreatography (ERCP): A retrospective study' [Heliyon Volume , Issue 5, 15 March 2024, Article e27447].

[This corrects the article DOI: 10.1016/j.heliyon.

Flaxseed Linusorb Alleviates Collagen-Induced Rheumatoid Arthritis in Rats via Inhibiting the TLR4/NF-κb/MAPK Signal Pathway and Modulating Gut Microbiota.

Flaxseed linusorb (FL) has gradually garnered widespread attention in recent years because of its intriguing bioactivities like anti-inflammation, antimelanogenesis, and even anticancer effects. Based on its proven anti-inflammatory activity and mechanism, it is supposed that FL may also exhibit an effect in treating and preventing rheumatoid arthritis (RA). Collagen-induced arthritis (CIA) rat models were established to investigate the potential therapeutic effect of FL, which were intervened with FL via gavage (50 and 100 mg/kg B.