Effects of Hypericum attenuatum Choisy extract on the immunologic function and intestinal microflora of broilers under oxidative stress.

This study investigated the impact of Hypericum attenuatum Choisy extract (HYG) on immunological function and the cecum microflora in broilers. A total of 240 one-day-old AA broilers were randomly divided into 5 groups with 6 replicates of 8 broilers each: 1) the CN group, in which broilers were injected with saline and fed a basal diet; 2) the PC group, in which broilers were injected with lipolyaccharide (LPS) and fed a basal diet; 3) the HYG1 group, in which broilers were injected with LPS and fed a 400 mg/kg HYG-supplemented diet; 4) the HYG2 group, in which broilers were injected with LPS and fed a 800 mg/kg HYG-supplemented diet; 5) the HYG3 group, in which broilers were injected with LPS and fed a 1,200 mg/kg HYG-supplemented diet. Broilers were injected with 1 mg/kg LPS or the same amount saline 12 hours before sampling on d 21 and 42.

Comparative transcriptomic analysis reveals differences in genes of different hypericum in Changbai Mountains.

To explore the differences between the hypericum in the Changbai Mountains, we carried out a transcriptome analysis of two common hypericums in the area, which was Choisy and Oliv. We screened the genes to analyze divergence time and evolutionary selection expression, and determine their expression levels. The results showed that we detected 9287 differentially expressed genes in the two species, of which shared 6044 genes by the two species.

Effects of plant growth regulators on the contents of rutin, hyperoside and quercetin in Hypericum attenuatum Choisy.

To explore the accumulation of rutin, hyperoside and quercetin in Hypericum attenuatum Choisy under treatment with different plant growth regulators, 100 mg/L, 200 mg/L and 300 mg/L cycocel, 100 mg/L, 200 mg/L and 300 mg/L mepiquat chloride and 1 mg/L, 2 mg/L and 3 mg/L naphthalene acetic acid were foliage sprayed on Hypericum attenuatum Choisy plants at the early growth stage. We sampled and determined the important flavonoid contents at the flowering stage. The results showed that the three plant growth regulators had different effects on the accumulation of rutin, hyperoside and quercetin in the leaves, stems and flowers of Hypericum attenuatum Choisy at the flowering stage.

Effects of dietary dandelion (Taraxacum mongolicum Hand.-Mazz.) polysaccharides on the performance and gut microbiota of laying hens.

This experiment was designed to evaluate the influences of dietary dandelion polysaccharides (DP) on the performance and cecum microbiota of laying hens. Three hundred laying hens were assigned to five treatment groups: the basal diet group (CK group), three DP groups (basal diets supplemented with 0.5, 1.

Phenolic composition, antioxidant, cytotoxic activities and cardioprotective effect of hydroalcoholic extract from aerial-parts of Fisch. ex Choisy.

This study investigated phenolic metabolites, antioxidant, cytotoxic and cardioprotective effects of the hydroalcoholic extract from the aerial parts of Fisch. ex Choisy. The total phenolic and flavonoid contents of the extract were 132.

Effects of inulin supplementation in laying hens diet on the antioxidant capacity of refrigerated stored eggs.

The effects of inulin supplementation in the diet of laying hens on the antioxidant capacity of refrigerated (0, 2, 4, 6 and 8 weeks) eggs were evaluated. Three hundred commercial laying hens were divided into five treatment groups and fed diets with supplements of 0 (control), 5, 10, 15 or 20 g/kg inulin. The experimental results showed that the use of inulin (15 or 20 g/kg) in the diet for laying hens increased the DPPH and ABTS radical-scavenging activities, total phenolic content and ferric reducing power in the yolks of fresh (stored 0 weeks) and stored eggs (P < 0.

[Changes of total flavonoids and rutin contents in different parts of wild and cultivated Hypericum attenuatum ].

Objective: To determine the content of total flavonoids and rutin in different parts of wild and cultivated Hypericum attenuatum.

Methods: Ethanol ultrasonic extraction method was used to extract total flavonoids and rutin in different parts of Hypericum attenuatum. With rutin as reference substance, UV spectrophotometry and high performance liquid chromatography were applied to deter- mine the total flavonoids and rutin content in Hypericum attenuatum samples (leaves, stems and flowers).

The effects of warming and nitrogen addition on soil nitrogen cycling in a temperate grassland, northeastern China.

Background: Both climate warming and atmospheric nitrogen (N) deposition are predicted to affect soil N cycling in terrestrial biomes over the next century. However, the interactive effects of warming and N deposition on soil N mineralization in temperate grasslands are poorly understood.

Methodology/principal Findings: A field manipulation experiment was conducted to examine the effects of warming and N addition on soil N cycling in a temperate grassland of northeastern China from 2007 to 2009.