A TaqMan probe-based multiplex real-time quantitative pcr for simultaneous detection of kobuvirus, parechovirus B, rosavirus B, and hunnivirus carried by murine rodents and shrews.

Background: Picornaviruses, common infectious agents in humans and various animal species, pose significant health threats. Conventional monoplex PCR is widely employed in laboratory diagnostics but is relatively time-intensive and laborious.

Results: In this study, we developed a multiplex TaqMan probe-based real-time quantitative PCR (qPCR) assay for the rapid and simultaneous detection of kobuvirus, parechovirus B, rosavirus B and hunnivirus in murine rodent and shrew samples.

Association between waist-to-hip ratio and testosterone in Chinese men with young-onset type 2 diabetes: a cross-sectional study.

Background: Low testosterone levels is associated with higher cardiovascular risk in men with diabetes. However, there are few studies on testosterone levels and the factors affecting them in patients with young-onset diabetes (YOD). The objective of this study was to investigate the correlation between waist-to-hip ratio (WHR) and testosterone levels in men diagnosed with YOD.

Preoperative classification of urinary stones based on community detection.

In the treatment of urinary stones, surgical intervention is crucial. Urinary stones composition and type directly affect surgical planning. However, research on preoperative stone composition analysis is limited.

Mulberry leaves supplementation modulates ruminal and fecal bacterial community and metabolites in growing mutton sheep.

Mulberry leaves, a traditional Chinese medicine with the values of medicine and food, are rich in various active ingredients and nutrients. Gastrointestinal bacteria are recognized to play a crucial role in food digestion, nutrient metabolism and the host immune health. However, the modulations of mulberry leaves on microbiota and metabolites remain insufficiently understood.

Host FSTL1 defines the impact of stem cell therapy on liver fibrosis by potentiating the early recruitment of inflammatory macrophages.

Adult stem cell therapy holds great promise for treating decompensated liver cirrhosis on the basis of animal studies, despite uncertainty about its clinical therapeutic efficacy and unclear underlying mechanisms. Here, we investigated the role of follistatin-like 1 (FSTL1), a profibrotic and proinflammatory matricellular protein, in inflammation-related heterogeneity in stem cell therapy. Our results showed that a high level of circulating FSTL1 is significantly correlated with therapeutic response in patients with cirrhosis.

[Comprehensive assessment of bronchial asthma: why it matters?].

Assessment of bronchial asthma is essential to optimize management and improve patient outcomes. A personalized, precision medicine approach, guided by comprehensive, accurate, and repeated assessments, is necessary to develop an individualized treatment strategy, to improve asthma control, and to achieve the best possible long-term outcomes, addressing the unique needs of each patient based on their asthma phenotype, endotype, severity, and response to treatment. Recent advances in research and technology have significantly diversified the methods of asthma assessment.

Green synthesis of polysaccharide-based Ag@AgCl photoactive porous hydrogel spheres for enhanced visible light photocatalytic degradation of organic pollutants.

Improving the visible light activity and recyclability of photocatalysts remains a key challenge for organic pollutant degradation in wastewater. In this study, enteromorpha polysaccharide (EP) and xanthan gum (XG) were combined to preload Ag ions, with CO bubbles introduced via a pressure-decompression method. After cross-linking with ZrOCl via drip infusion, porous hydrogel spheres embedded with photoactive Ag@AgCl nanoparticles (NPs) were obtained.

The heading stage: A critical period for schwertmannite in reducing arsenic accumulation in rice (Oryza sativa L.).

Schwertmannite, a common iron-derived mineral, is known for its high efficiency in adsorbing As from water and reducing the mobility and availability of As in soils. However, few studies have examined the critical period for the effectiveness of schwertmannite in hindering As uptake by rice plants, particularly its impact on the uptake and transport of As across different growth stages of rice. In this study, hydroponic experiments were performed to explore the absorption and translocation of As (500 µg/L As(III) or As(V)), when combined with schwertmannite, in rice during all growth stages.

Molecular insights into the simultaneous removal mechanisms of As(Ⅴ) and Cd(II) in iron tailings slag-biochar composites.

Arsenic and cadmium contamination frequently coexist in the real environment. However, it remains a challenge for their simultaneous removal due to their distinct physicochemical properties at low cost. To this end, a cost-effective magnetic biochar adsorbent (ITBNa800) was prepared using biomass waste and iron tailings slag.

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Transcriptomic and proteomic analyses reveals the diverse venom composition of the spider Neoscona shillongensis.

Neoscona shillongensis (Araneidae), also known as the Shillong orb-weaving spider, is a species that constructs a typically shaped round web, is inactive during the daytime and comes out at dusk to forage, primarily preying on flying insects such as mosquitoes, flies, and moths. This spider serves as an important natural enemy of agricultural and forest pests. Neoscona shillongensis primarily uses its web to capture prey, but its venom also plays a crucial role in subduing and processing prey.

Kidney-targeting DNA tetrahedral molecular cage synergistically inhibits acute kidney injury by clearing ROS and activating HO-1.

Acute kidney injury (AKI) is a major cause of mortality in hospitalized patients, yet effective therapeutic interventions remain underdeveloped. To address this critical need, we have employed tetrahedral framework nucleic acid (tFNA) as a carrier to self-assemble a complex incorporating G-quadruplex and hemin (G4/Hemin). This novel formulation exhibits uniform particle size, targeted delivery, and significant therapeutic efficacy for AKI.

Micron-scale heterogeneity reduction leads to increased interspecies competition in thermophilic digestion microbiome.

Microbial spatial heterogeneity is an important determinant of larger-scale community properties, whereas most studies neglect it and therefore only provide average information, potentially obscuring the signal of microbial interactions. Our study takes a step toward addressing this problem by characterizing the spatial heterogeneity of a microbiome with micron-scale resolution. Micron-scale single clusters (40-70 μm) were randomly collected from lab-scale anaerobic digestion (AD) biosystems, and a comparative analysis was performed to evaluate differences between mesophilic and thermophilic systems.

Transplant Outcomes Using Older Matched Sibling Donors Compared to Young Alternative Donors: A CIBMTR Analysis.

Whether younger donors should be prioritized over HLA-matching for allogeneic hematopoietic cell transplantation (allo-HCT) when using post-transplant cyclophosphamide (PTCy)-based graft versus host disease (GvHD) prophylaxis is unclear. To address this, we compared outcomes of allo-HCT recipients aged ≥50-years using PTCy-based GvHD prophylaxis from an older (≥50-years) matched sibling donor (MSD) to those of younger alternative donors ≤35-years: HLA-matched unrelated donors (MUD), HLA-mismatched unrelated donors (MMUD), and haploidentical (haplo)-related donors reported to the Center for International Blood & Marrow Transplant Research between 2014-2021. Young MUD and older MSD receiving calcineurin inhibitor (CNI)-based allo-HCT that met study criteria were concurrently examined.

Aggressive Ablation versus Regular Ablation for Persistent Atrial Fibrillation: A Multicenter Real-World Cohort Study.

Background And Aims: Current guidelines for the optimal ablation strategy for persistent atrial fibrillation(PerAF) remain unclear. While our previous RCT confirmed the favorable prognosis of aggressive ablation, real-world evidence is still lacking.

Methods: Among 4,833 PerAF patients undergoing catheter ablation at 10 centers, two groups were defined: Regular Ablation(PVI-only or PVI plus anatomical ablation) and Aggressive Ablation(anatomical plus electrogram-guided ablation), with 1,560 patients each after propensity score(PS) matching.

Pregnancy Metal Mixtures and Blood Pressure and Hypertension in Mid-Life: A Prospective U.S. Cohort Study.

Background: The long-term associations between metal mixtures in pregnancy and women's mid-life blood pressure (BP) and hypertension remain unclear.

Methods: In Project Viva (enrolled 1999-2002), we measured nonessential (arsenic, barium, cadmium, cesium, mercury, lead) and essential metals (copper, magnesium, manganese, selenium, zinc) in red blood cells, along with folate and vitamin B12 in plasma, collected during pregnancy. We measured mid-life BP from 2017 to 2021 (median age, 51.

Design, Synthesis, and Herbicidal Activity of Biaryl-Pyridazinone/Phthalimide Derivatives as Novel Protoporphyrinogen Oxidase Inhibitors.

In this study, a series of biaryl-pyridazinone/phthalimide derivatives were designed and synthesized as novel protoporphyrinogen IX oxidase (PPO) inhibitors. Herbicidal activity and crop safety assessments revealed that some compounds exhibited excellent herbicidal activity and crop safety profiles. For instance, at 37.

Assessment of Syphilitic Optic Neuropathy: A Prospective Cross-Sectional Study.

Purpose: To evaluate the clinical features of syphilis optic neuropathy (SON) and explore its underlying pathogenesis by measuring the peripapillary retinal nerve fiber layer (pRNFL).

Methods: This was a prospective cross-sectional study. Syphilitics, regardless of ocular complaints, in the ophthalmology department in 2020 were screened for SON, which was defined as isolated optic neuropathy without overt signs of syphilitic uveitis.

Mandrel-free fabrication of giant spring-index and stroke muscles for diverse applications.

Methods for making high-spring-index polymer fiber or yarn muscles have required expensive fabrication by wrapping around a mandrel, which limits their practical applications. We demonstrate an inexpensive mandrel-free method for making polymer muscles that can have a spring index of >50 and a contractile tensile stroke exceeding 97%. This method enables the spring index to be varied along a muscle's length by varying the plying twist, resulting in muscles that transition between homochiral and heterochiral when either heated or cooled.

Flexible Organic-Polyphosphates Interfacial Layer for Stable Lithium Metal Anode.

Lithium (Li) metal is regarded as a desired anode candidate for high-energy-density rechargeable battery systems in the future because of its high specific capacity and low redox potential. However, Li dendritic growth and volume expansion during cycling severely hinder its practical application. Herein, an artificial organophosphorus-inorganic Li hybrid flexbile solid electrolyte interphase (SEI) layer was designed by a prereaction between phytic acid (PA) and lithium hydroxide (LiOH) to generate metal chelates for quick Li conductivity.

Graph Foundation Models: Concepts, Opportunities and Challenges.

Foundation models have emerged as critical components in a variety of artificial intelligence applications, and showcase significant success in natural language processing and several other domains. Meanwhile, the field of graph machine learning is witnessing a paradigm transition from shallow methods to more sophisticated deep learning approaches. The capabilities of foundation models in generalization and adaptation motivate graph machine learning researchers to discuss the potential of developing a new graph learning paradigm.

An Intelligent Grading Model for Myopic Maculopathy Based on Long-Tailed Learning.

Purpose: To develop an intelligent grading model for myopic maculopathy based on a long-tail learning framework, using the improved loss function LTBSoftmax. The model addresses the long-tail distribution problem in myopic maculopathy data to provide preliminary grading, aiming to improve grading capability and efficiency.

Methods: This study includes a data set of 7529 color fundus photographs.

Clinical and genetic characteristics of glucose transporter 1 deficiency syndrome in a large cohort of Chinese patients.

Background: Mutations in the SLC2A1 gene cause glucose transporter type 1 deficiency syndrome (Glut1DS). This study aimed to investigate the clinical and molecular genetics characteristics of Chinese patients with Glut1DS.

Methods: The clinical data of patients with Glut1DS were analyzed retrospectively.

Propulsion Mechanisms in Magnetic Microrobotics: From Single Microrobots to Swarms.

Microrobots with different structures can exhibit multiple propulsion mechanisms under external magnetic fields. Swarms dynamically assembled by microrobots inherit the advantages of single microrobots, such as degradability and small dimensions, while also offering benefits like scalability and high flexibility. With control of magnetic fields, these swarms demonstrate diverse propulsion mechanisms and can perform precise actions in complex environments.

Recent Advances in AlN-Based Acoustic Wave Resonators.

AlN-based bulk acoustic wave (BAW) filters have emerged as crucial components in 5G communication due to their high frequency, wide bandwidth, high power capacity, and compact size. This paper mainly reviews the basic principles and recent research advances of AlN-based BAW resonators, which are the backbone of BAW filters. We begin by summarizing the epitaxial growth of single-crystal, polycrystalline, and doped AlN films, with a focus on single-crystal AlN and ScAlN, which are currently the most popular.