ESM1 suppresses LncRNA GAS5/miR-23a-3p/PTEN axis to promote the cisplatin-chemotherapy resistance of ovarian cancer cells via activating the PI3K/AKT pathway.

Background: Cisplatin chemotherapy is an important treatment for advanced ovarian cancer (OC). However, the development of cisplatin resistance greatly limits the survival time of OC patients. Endothelial cell-specific molecule 1 (ESM1) has been found to be an important proto-oncogene promoting OC, but its mediating OC cisplatin resistance remains unknown.

Efficacy of a combination of O-RADS, CEUS, and CA125, in identification of ovary-adnexal malignant lesions.

Objectives: To evaluate the efficacy of a combination of Ovarian-Adnexal Reporting and Data System (O-RADS), contrast-enhanced ultrasound (CEUS), and cancer antigen 125 (CA125) in identifying malignant ovarian-adnexal lesions and improving diagnostic accuracy, providing a reliable reference for the evaluation and management of ovarian-adnexal malignancies to enhance patient prognosis.

Methods: The study analyzed the diagnostic performance of O-RADS alone and in combination with CEUS and CA125 for distinguishing between benign and malignant ovarian-adnexal lesions. Sensitivity, specificity, accuracy, and Kappa values were calculated to assess the efficacy of these diagnostic approaches.

Identification and mechanism analysis of biomarkers related to butyrate metabolism in COVID-19 patients.

Background: Butyrate may inhibit SARS-CoV-2 replication and affect the development of COVID-19. However, there have been no systematic comprehensive analyses of the role of butyrate metabolism-related genes (BMRGs) in COVID-19.

Methods: We performed differential expression analysis of BMRGs in the brain, liver and pancreas of COVID-19 patients and controls in GSE157852 and GSE151803.

Direct recovery of high-purity uranium from fluoride-containing nuclear wastewater via extraction materials with ensemble Lewis sites and a tandem electrochemical device.

Electrochemical uranium extraction from real nuclear wastewater with a high concentration of fluoride ions (F-) represents a promising strategy for the efficient treatment of radioactive wastewater and the recovery of the valuable uranium resource. However, the current progress suffers from the interference of extremely high concentration of F- and undesired purity of the final uranium product. Herein, we constructed the neighboring ensemble Lewis acid-base pair sites (ensemble Lewis sites) in bismuth oxides as the extraction material, which was integrated into a designed tandem electrochemical device for efficient recovery of high-purity uranium from real nuclear wastewater.

CAD manipulates tumor intrinsic DHO/UBE4B/NF-κB pathway and fuels macrophage cross-talk, promoting hepatocellular carcinoma metastasis.

Background And Aims: Portal vein tumor thrombosis (PVTT), an indicator of clinical metastasis, significantly shortens hepatocellular carcinoma (HCC) patients' lifespan, and no effective treatment has been established. We aimed to illustrate mechanisms underlying PVTT formation and tumor metastasis, and identified potential targets for clinical intervention.

Approach And Results: Multi-omics data of 159 HCC patients (including 37 cases with PVTT) was analyzed to identify contributors to PVTT formation and tumor metastasis.

The Effect of Electroencephalographic Biofeedback Therapy on Anxiety and Overall Well-being in Patients with Rectal Cancer.

This study aimed to investigate the effectiveness of electroencephalographic (EEG) biofeedback therapy in reducing anxiety levels and improving overall well-being among patients diagnosed with rectal cancer. A randomised controlled trial was conducted with 150 patients with rectal cancer who were randomly assigned to either the intervention group (n = 75) or the control group (n = 75). The intervention group received 16 sessions of EEG biofeedback therapy over 8 weeks, whereas the control group received standard care.

Oral hygiene is independently associated with early stroke-associated pneumonia in hospitalized patients with ischemic stroke: An observational study.

Stroke-associated pneumonia (SAP) is a major stroke complication. Oral microorganisms are important contributors to SAP. Here, we aimed to investigate whether oral hygiene is associated with early SAP (<72 h of stroke onset) in patients with ischemic stroke.

Optimization of enzyme-ultrasound assisted extraction from mulberries anthocyanins based on response surface methodology and deep neural networks and analysis of in vitro antioxidant activities.

This study used Xinjiang native "medicinal and food dual-use" resource mulberries as raw material, and optimized the extraction process of mulberries anthocyanins by enzyme-ultrasound-assistance through the establishment of a response surface model (RSM) and deep neural network model (DNN). A single-factor-Box-Behnken experiment was conducted to investigate the effects of pectinase dosage, enzymatic hydrolysis time, ultrasonic temperature, ultrasonic time, solvent concentration, and solid-liquid ratio on the extraction rates of total anthocyanins (TAC), and cyanidin-3-O-glucoside (C3G), cyanidin-3-O-rutinoside (C3R) two anthocyanin compounds, and the comprehensive evaluation index was used as a reference to obtain the optimal extraction conditions. The results show that both the RSM and DNN models could predict accurately, but by comparing the coefficient of determination (R) of the two models, it was found that the DNN model (R = 0.

Understanding benefit finding among burn survivors: A latent profile analysis study.

Objective: This study aimed to utilize Latent Class Analysis to identify distinct benefit finding traits among burn survivors, analyze the differences in benefit finding between subgroups based on their demographic and clinical characteristics, and explore the variables that are associated with these distinct patterns.

Method: From June 2022 to December 2023, a self-reported cross-sectional survey was conducted in Northeast China to investigate benefit finding among burn survivors. The survey included measures of demographic, clinical, specific emotion, and social isolation variables.

The potential value of microRNA-409-5p-mediated negative regulation of USP7 in the diagnosis and treatment of acute myocardial infarction.

Purpose: This study aimed to explore the effects of ubiquitin-specific peptidase 7 (USP7) on acute myocardial infarction (AMI) and the negative regulation of USP7 by microRNA-409-5p (miR-409-5p).

Methods: Clinical data were collected from patients admitted to the Cardiology Department of Yan'an Hospital of Kunming City between July 2020 and July 2021. The participants included patients with AMI (AMI; n = 30), stable angina pectoris (SAP; n = 30), and chest pain syndrome (CPS; n = 30) and healthy controls (n = 30).

Ferrostatin-1 reduces the inflammatory response of rheumatoid arthritis by decreasing the antigen presenting function of fibroblast-like synoviocytes.

Rheumatoid arthritis (RA) is a systemic chronic autoimmune disease with complex mechanism. Currently, ferroptosis is believed to play a role in it, but the specific mechanism is unknown, especially in immune response. In this study, we demonstrated that the high expression of major histocompatibility complex I (MHC-I) molecules in RA fibroblast-like synoviocytes (FLSs) is an antigen-presenting cell property and that this property is closely related to the increase in antigens after citrullination.

Discovery of soluble epoxide hydrolase inhibitors from Inula britannica: Inhibition kinetics, molecular dynamics simulation, biochemical, and in vitro cell-based studies.

Soluble epoxide hydrolase (sEH) is an enzyme involved in transforming epoxyeicosatrienoic acids (EETs) to dihydroxyeicosatrienoic acids (DHETs). EETs play a pivotal role in maintaining anti-inflammatory response and pain regulation, making sEH as an important target for therapeutic interventions. Based on our present study of discovery of sEH inhibitors, herein, six new (1-6) and fifteen known compounds (7-21) with inhibitory effects toward sEH were isolated from Inula britannica.

Effect of Yangling inclined trellis tree shape on light interception efficiency, fruit quality, and yield of sweet cherry cv: 'Jimei'.

Different tree shapes (TSs) directly influence canopy structure, light interception, and photosynthetic activity, impacting fruit quality and yield. This study investigated the effects of light interception efficiency (LIE) on the quality and yield of "Jimei" sweet cherry fruits by comparing two tree shapes: the Yangling inclined trellis arm TS (YLL-TS) and the Super slender spindle TS (SSS-TS). Using three-dimensional digitization techniques, we analyzed the growth relationships between branches and leaves, constructed virtual canopy models, and examined branch composition, leaf area, and spatial distribution.

Assembly of a lignocellulose-degrading synthetic community from the strong-flavor Daqu by a stepwise method.

The lignocellulose in Daqu plays an important role during the Baijiu fermentation, such as providing energy for microbial metabolism and precursors for flavor compounds. However, due to the complexity of the Daqu microbial community and the fermentation environment, the regulation of lignocellulose degradation efficiency is limited. In such cases, artificial intervention can be achieved through the application of synthetic communities.

Mussel Foot Protein Membrane-Enclosed Crystalline Drug with Zero-Order Release Kinetics for Long-Acting Therapy.

Injectable formulations with sustained and steady release capabilities are critically required to treat diseases requiring temporary or lifelong continuous therapy, especially for drugs with a short half-life. Additionally, achieving a sufficiently high drug loading in a single dose remains a persistent challenge. Herein, by mimicking the formation principles of mussel adhesive plaques, we have developed membrane-enclosed crystalline systems of insulin and progesterone as model macro- and small-molecular crystalline drugs.

Photo-crosslinking injectable Photothermal antibacterial hydrogel based on quaternary ammonium grafted chitosan and hyaluronic acid for infected wound healing.

Antibacterial hydrogels not only provide a better environment for skin wounds to avoid infection but also accelerate wound healing. Herein, chitosan modified by a quaternary ammonium salt (CQ), and hyaluronic acid grafted with methacrylate (HM) were designed and synthesized to prepare an injectable photo-crosslinking hydrogel for wound dressing with inherent antibacterial and photothermal properties. CQ and HM exhibited excellent biocompatibility, improved water retention, and antibacterial activity, illustrating vast potential as an antibacterial material in various applications.

3D printing of microstructured polyacrylamide/sodium alginate/lithium chloride composite hydrogels for nanofriction generator and e-skin.

The development of wearable electronic devices presents significant challenges to develop flexible power supplies and sensors that are efficient and convenient, highly sensitive, and adaptable to complex environments. In this study, dual-network ionic PSL hydrogels were successfully synthesized and characterized, which have excellent cyclic tensile properties (maximum stress fluctuation <8 % over 50 tensile times), anti-freezing (-47.2 °C), electrical conductivity (175.

Antifungal effect and control of Alternaria fruit rot in blueberry through ε-polylysine hydrochloride.

Blueberry was easily infected by Alternaria sp. during storage time, leading to Alternaria fruit rot (AFR). ε-polylysine hydrochloride (ε-PL), a widely used food additive, was evaluated for its antifungal efficacy at different concentrations and its effects on the quality of blueberries treated with 3 g L ε-PL.

A bioinspired polymeric membrane-enclosed insulin crystal achieves long-term, self-regulated drug release for type 1 diabetes therapy.

The nuclear envelope serves as a highly regulated gateway for macromolecule exchange between the nucleus and cytoplasm in eukaryotes. Here we have developed a cell nucleus-mimicking polymeric membrane-enclosed system for long and self-regulated therapy. A polymeric nano-membrane with nanopores is conformally synthesized in situ on the surface of each insulin crystal, ensuring sustained, adjustable and zero-order drug release kinetics.

Metabolites Discovery from : Exploring the Potential of Desert Microorganisms.

The Taklamakan Desert is an extreme environment supporting a unique and diverse microbial community with significant potential for exploration. Strain TRM70308, isolated from desert soil, shares 98.43% 16S rRNA gene sequence similarity with OF1.

Historical loss weakens competitive behavior by remodeling ventral hippocampal dynamics.

Competitive interactions are pervasive within biological populations, where individuals engage in fierce disputes over vital resources for survival. Before the establishment of a social hierarchy within the population, this competition becomes even more intense. Historical experiences of competition significantly influence the competitive performance; individuals with a history of persistent loss are less likely to initiate attacks or win escalated contests.

Targeted delivery of CCL3 reprograms macrophage antigen presentation and enhances the efficacy of immune checkpoint blockade therapy in hepatocellular carcinoma.

Background: Hepatocellular carcinoma (HCC) remains a leading cause of cancer-related deaths worldwide, especially in advanced stages where limited treatment options result in poor prognosis. The immunosuppressive tumor immune microenvironment (TIME), characterized by low immune cell infiltration and exhaustion, limits immunotherapy efficacy. To address this, our study investigates the role of C-C motif chemokine ligand 3 (CCL3) in modulating the HCC TIME.

A comparative study of the composition of microorganisms and metabolites in different β-casein genetic types of dairy cows based on metagenomics and non-targeted metabolomics.

β-Casein is the main component of cow's milk protein, with A1 and A2 β-casein being the most common. Of these, A1 β-casein hydrolysate produces BCM-7, which can cause lactose intolerance, while A2 β-casein milk is more gentle on the gut. However, there is limited research on the composition of rumen microbiota, metabolites, and host metabolites in different genotype cows using metagenomics and metabolomics.

Hybrid EMT Phenotype and Cell Membrane Tension Promote Colorectal Cancer Resistance to Ferroptosis.

Intratumoral heterogeneity, including epithelial-mesenchymal transition (EMT), is one major cause of therapeutic resistance. The induction of ferroptosis, an iron-dependent death, has the potential in overcoming this resistance to traditional treatment modalities. However, the roles of distinct EMT phenotypes in ferroptosis remain an enigma.

How urbanization shapes the effectiveness of school closures on hand, foot, and mouth disease.

Objective: This study analyzed the influence of urbanization on the effectiveness of school closures in controlling hand, foot, and mouth disease (HFMD).

Methods: Data were collected from 31 provinces in mainland China between 2010 and 2019. The effect of school closures on HFMD was estimated and synthesized for high and low urbanization groups.