[Corneal permeability of topically applied pirenzepine solution].

Objective: To study the corneal permeability of three different pirenzepine eye-drop solutions and provide reference for further clinical use.

Methods: Sixty-three New Zealand white rabbits were divided into three groups. Each group of rabbits received 2% pirenzepine (pirenzepine group), 2% pirenzepine with 0.

[Relationship between pterygium onset and ultraviolet rays exposure time].

Objective: To investigate the dose-effect relationship between the radiation of ultraviolet ray and the onset of pterygium quantitatively.

Methods: Interrogation was conducted to 95 patients with pterygium in Yacheng district, Sanya City, Hainan Province, 37 males and 58 females, aged 55.7 +/- 13.

[Enhanced coagulation and NOM fractionation study of a typical southern water].

Systematic studies on enhancing normal treatment processes were performed in order to remove aquatic organic matter economically and efficiently. Enhanced coagulation and polymer coagulant-aid coagulation were compared through jar tests and on-spot experiment (Pilot-scale and full-scale experiment). Fractionations of raw water and water after treatments of above two strategies were performed based on selective adsorption of resin DAX-8.

[Changes of expression of the P38 MAPK and caspase-3 in rat retinal ischemia-reperfusion model and the influence of Nimodipine].

Objective: To investigate the protective effects of L-type calcium blocker Nimodipine on retinal ischemia-reperfusion of rat and its affect on cell signal transduction system.

Method: Ninety-five Wistar rats were divided into 4 groups randomly. Group A was normal (blank) control group and included 5 rats; Group B was retinal ischemia-reperfusion (experimental control) group which had 30 rats; Group C was retinal ischemia-reperfusion plus Nimodipine (experimental) group that included 30 rats; and group D was low-pressure irrigation (pseudo-operation) group that had 30 rats.

[Development of children's self-regulation competence and its relationship with behavioral problems].

Objective: To study the development of self-regulation competence and its relationship with behavioral problems in Chinese children with the method of questionnaire assessment.

Methods: The subjects of the study consisted of 18 to 36 months old toddlers and 3 to 6 years old children. Self-regulation competence in this study included effortful control and self-regulation development.

[Association between vitamin D receptor gene polymorphism and vitamin D deficiency rickets].

Objective: To explore the genetic susceptibility of children to vitamin D deficiency rickets through studying the association between Vitamin D receptor (VDR) gene polymorphism and vitamin D deficiency rickets.

Methods: One hundred and fifty-nine children (100 boys and 59 girls, aged 0 to 2 years), with new-onset vitamin D deficiency rickets were enrolled. The patients sampled from a community of Jiamusi City, Heilongjiang Province.

MRI features of pediatric cerebral paragonimiasis in the active stage.

We retrospectively reviewed the MR images of the brains of six children (age = 5-13 years) who had cerebral paragonimiasis in the early active stage. Diagnosis was based on a positive antibody test enzyme-linked immunosorbent assay (ELISA) for paragonimiasis in serum. The most common finding (in five patients) was irregular hemorrhage of various degrees.

Removal of natural organic matter in a typical south-China source water during enhanced coagulation with IPF-PACl.

Systematic investigation on enhancing removal of natural organic matter (NOM) using inorganic polymer flocculant (IPF), polyaluminum chloride (PACl) and polyacrylamide (PAM) was performed in a typical south-China source water. Enhanced coagulation and applying polymer flocculant-aid were compared through jar tests and pilot tests. Raw water and settled water were characterized and fractionated by resin adsorption.

OsGLU1, a putative membrane-bound endo-1,4-beta-D-glucanase from rice, affects plant internode elongation.

A dwarf mutant glu was identified from screening of T-DNA tagged rice population. Genetic analysis of the T1 generation of glu revealed that a segregation ratio of wild-type:dwarf phenotype was 3:1, suggesting that the mutated phenotype was controlled by a single recessive nuclear locus. The mutated gene OsGLU1, identified by Tail-PCR, encodes a putative membrane-bound endo-1,4-beta-D-glucanase, which is highly conserved between mono- and dicotyledonous plants.

Roles of ethylene receptor NTHK1 domains in plant growth, stress response and protein phosphorylation.

Ethylene receptors sense ethylene and regulate downstream signaling events. Tobacco ethylene receptor NTHK1, possessing Ser/Thr kinase activity, has been found to function in plant growth and salt-stress responses. NTHK1 contains transmembrane domains, a GAF domain, a kinase domain and a receiver domain.

Cloning and comparative analysis of the gene encoding diacylglycerol acyltransferase from wild type and cultivated soybean.

Diacylglycerol acyltransferase (DGAT), as an important enzyme in triacylglycerol synthesis, catalyzes the final acylation of the Kennedy pathway. In the present study, the GmDGAT gene was cloned from Glycine max by using AtDGAT as a query to search against the soybean EST database and the rapid amplification of cDNA ends (RACE) method. Allelic genes were also isolated from 13 soybean accessions and the divergence of the deduced amino acid sequences were compared.

Genome-wide profiling of segmental-regulated transcriptomes in human epididymis using oligo microarray.

Sperm maturation during passage through the epididymis depends on regionalized gene expression which maintains the progressively changing environment within the epididymal tubule. Towards defining the genes that drive the sequential maturation of spermatozoa, we profiled regionally regulated gene expression pattern in the epididymis of a fertile young male donor using Affymetrix human genome U133 plus 2.0 microarray representing approximately the whole human genome.

[Toxicity and influencing factors of liquid chlorine on chironomid larvae].

The excessive propagation of Chironomid larvae (red worm) in the sedimentation tanks is a difficult problem for the normal function of waterworks. The toxic effect of liquid chlorine on the different instar larvae of Chironomid was studied using distilled water as test sample. Furthermore, the effect of pH value, organic matter content, ammonia nitrogen, and algae content on toxicity of liquid chlorine was observed.

AtNAC2, a transcription factor downstream of ethylene and auxin signaling pathways, is involved in salt stress response and lateral root development.

An NAC-type transcription factor gene AtNAC2 was identified from Arabidopsis thaliana when expression patterns of the genes from a microarray analysis were examined. The AtNAC2 expression was induced by salt stress and this induction was reduced in magnitude in the transgenic Arabidopsis plants overexpressing tobacco ethylene receptor gene NTHK1. AtNAC2 is localized in the nucleus and has transcriptional activation activity.

Removal of disinfection by-products formation potential by biologically intensified process.

The removal of disinfection by-products formation potential (DBPFP) in artificially intensified biological activated carbon (IBAC) process which is developed on the basis of traditional ozone granular activated carbon was evaluated. By IBAC removals of 31% and 68% for THMFP and HAAFP were obtained respectively. Under identical conditions, the removals of the same substances were 4% and 32% respectively only by the granular activated carbon (GAC) process.

Functional analysis of a putative Ca2+ channel gene TaTPC1 from wheat.

The cytosolic free-calcium concentration [Ca2+](cyt) transiently increases under abiotic stresses and the proteins that control this process are gradually disclosed. The Ca2+-permeable channel is one type of these proteins in plants. In the present study, a novel Ca2+-permeable channel gene TaTPC1 encoding a putative membrane protein was cloned from wheat.

A putative plasma membrane cation/proton antiporter from soybean confers salt tolerance in Arabidopsis.

Cation transport is thought to be an important process for ion homeostasis in plant cells. Here, we report that a soybean putative cation/proton antiporter GmCAX1 may be a mediator of this process. GmCAX1 is expressed in all tissues of the soybean plants but at a lower level in roots.

[Anxiety state and its related factors in Shanghai high school students].

Objective: To investigate the anxiety state in high school students and related factors in order to get reasonable suggestions for prevention.

Methods: The mental health test (MHT) for high school students, and the living environmental and parental style were used in this study.

Results: MHT served as an assessment scale of anxiety.

Flocculation control study based on fractal theory.

A study on flocculation control based on fractal theory was carried out. Optimization test of chemical coagulant dosage confirmed that the fractal dimension could reflect the flocculation degree and settling characteristics of aggregates and the good correlation with the turbidity of settled effluent. So that the fractal dimension can be used as the major parameter for flocculation system control and achieve self-acting adjustment of chemical coagulant dosage.

[The effect of UV-irradiation on telomerase activity and other stress-related proteins in human lens epithelial cells].

Objective: To investigate the changes and the role of telomerase activity and other stress-related proteins in the process of UV-induced DNA damage and repair in human lens epithelial cells.

Method: Human lens epithelial cells were irradiated at UV-doses 0.0 (control group) and 0.

[Cytokine expression in murine cornea tissue during recurrent herpetic stromal keratitis].

Objective: To investigate the expression of cytokine in CD(4)(+) T helper type 1 (Th1) and T helper type 2 (Th2) cells in murine cornea during recurrent herpetic stromal keratitis (HSK) and its relationship with the HSK.

Methods: Seven weeks after corneal inoculation with HSV-1, the eyes of latently infected BALB/c mice were irradiated by UV-B and examined for the signs of inflammation and viral reactivation. The corneas of six mice with recurrent stromal disease and six controls were examined to detect the content of Th1 type cytokines (IFN-gamma and IL-12) and Th2 type cytokines (IL-4 and IL-10) on days 0, 3, 7, 10, 14, 21 and 28 after irradiation.

[Laminin and fibronectin influence growth characteristics of human lens epithelial cells in vitro].

Objective: To investigate the effects of Laminin (Ln) and Fibronectin (Fn) on human lens epithelial cells (hLECs) in vitro.

Methods: Twenty-eight pieces of lens anterior capsule were cut into scraps and seeded on dishes coated with Ln, Fn or BSA. The morphological characters of the hLECs were detected with an inverse microscope.

Soybean DRE-binding transcription factors that are responsive to abiotic stresses.

Three DREB homologue genes, GmDREBa, GmDREBb, and GmDREBc, were isolated from soybean, Glycine max (L.) Merr. Each of the deduced proteins contains an AP2 domain of 64 amino acids.

[The protective effect of recombinant adenovirus of catalase on oxidative damaged rat lens].

Objectives: To investigate the validity of catalase recombinant adenovirus on the treatment of oxidative cataract.

Methods: The coding sequence of catalase was cloned and the catalase recombinant adenovirus was constructed. The expression time course of catalase gene in rat lens infected by recombinant adenovirus was determined by Western blotting.