High-temperature crystal structures and chemical modifications in RbH(2)PO(4).

We have used laboratory and synchrotron x-ray diffraction to investigate the structural and chemical changes undergone by polycrystalline RbH(2)PO(4) upon heating within the 30-250 °C temperature interval. Our data show no evidence of the previously reported onset of partial polymerization at T = 96 °C (Park et al 2001 J. Phys.

[The effect of form of alveolar ridge on relining of complete dentures].

Purpose: To evaluate the effect of relining on complete dentures by analysis of model measurement.

Methods: 15 edentulous mandibular models were fabricated by close mouth impression, these models were marked and cut at 5 different positions, the alveolar height, width and sectional area were measured, then direct relining was performed on these patients' lower denture (Tokuyama Rebase Fast II). The cases were followed up for 7 days.

[Biological and molecular characteristics of a cat-borne Bartonella clarridgeiae].

Objective: To characterize a Bartonella strain M9HN-SHQ from a blood culture of cat from Henan Province,China.

Methods: The organisms were subcultured in 5% CO2 at 37 degrees C on trypticase soy agar containing 5% sheep blood for 6 to 7 days. We analyzed the isolate using whole-cell fatty acid analysis,Etest for susceptibility testing, random amplified polymorphic DNA (RAPD), pulsed-field gel electrophoresis(PFGE) and sequence analysis of 16S rRNA, gltA, groEL, ftsZ, rpoB, ribC and 16S-23S rRNA intergenic spacer region.

Prevalence and characterization of heterogeneous vancomycin-intermediate Staphylococcus aureus isolates from 14 cities in China.

The prevalence of heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) among 1,012 vancomycin-susceptible methicillin (meticillin)-resistant S. aureus isolates collected from 14 cities in China from 2005 to 2007 was 13 to 16%, as determined by a combination of (i) measurement by the modified population analysis profile-area under the curve method (PAP-AUC) and (ii) estimation from the measured sensitivity and specificity of a screening method. Two hundred isolates from blood were chosen as a subset for measurement of the sensitivities and the specificities of several previously described screening methods by using the results of PAP-AUC as the reference.

Toxoplasma gondii: proteomic analysis of antigenicity of soluble tachyzoite antigen.

The obligate intracellular parasite Toxoplasma gondii is an important pathogen of humans and animals. The tachyzoite of T. gondii is the main life-cycle stage that is responsible for toxoplasmosis.

[Multilocus sequence typing of methicillin-resistant Staphylococcus aureus].

Objective: To analyze multilocus sequence typing (MLST) of methicillin-resistant Staphylococcus aureus (MRSA) strains in 2000 and 2005, and get a primary knowledge of MLST Characterization of MRSA.

Methods: Sequence analysis was conducted on seven allelic genes of 29 methicillin-resistant Staphylococcus aureus strains and 2 methicillin-sensitive Staphylococcus aureus (MSSA) strains and the allelic profiles were gained from internet database.

Results: All 12 MRSA strains in 2000 were sequence type (ST) 239 and 10 MRSA strains in 2005 were ST239, while 7 MRSA strains in 2005 were new types, ST5 (41.

[Detection of tissue protein biomarkers by surface enhanced laser desorption/ionization-time of flight-mass spectrometry in patients with mycosis fungoides].

Objective: To search the tissue biomarker in mycosis fungoides (MF) patients by surface enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS).

Methods: Skin specimens were collected from 12 MF patients and 15 normal controls to prepare solution of protein. CM10 Proteinchip was used to detect the biomarkers for MF.

Comparative proteomic analysis of passaged Helicobacter pylori.

In order to identify the proteins associated with Helicobacter pylori colonization in mice, we used 2-dimensional gel electrophoresis (2-DE) to analyze the membrane- and soluble-cellular proteins extracted from H. pylori strain 26695 and the mouse-passaged homolog 88-3887. We defined 2- and 3-fold changes in protein expression as the threshold values for differential expression in the membrane-protein and whole-cell-protein fractions, respectively.

[The application of case-based learning in English in teaching of clinical stomatological courses].

As a medical course approach of posing contextualized questions that are based upon "real life" clinical problems, case-based learning (CBL) is used to stimulate and underpin the acquisition of knowledge, skills, and attitudes by clinical cases. Good effects were acquired in the practice of CBL teaching in English applied by College of Stomatology, Shanghai Jiao Tong University. Responses from both students and teachers reflected that this course approach won their high acceptability, and was worthy of use in selected stomatological courses.

Regularizing method for the determination of the backscatter cross section in lidar data.

The retrieval of the backscatter cross section in lidar data is of great interest in remote sensing. For the numerical calculation of the backscatter cross section, a deconvolution has to be performed; its determination is therefore an ill-posed problem. Most of the common techniques, such as the well-known method of Gaussian decomposition, make implicit assumptions on both the emitted laser pulse and the scatterers.

Wavelength division multiplexing of chaotic secure and fiber-optic communications.

Wavelength division multiplexing (WDM) transmission of chaotic optical communication (COC) and conventional fiber-optic communication (CFOC) is numerically confirmed and analyzed. For an 80-km-long two-channel communication system, a 1-Gb/s secure message in COC channel and 10-Gb/s digital signal in CFOC channel are simultaneously achieved with 100 GHz channel spacing. Our numerical simulations demonstrate that the COC and CFOC can realize no-crosstalk transmission of 80 km when the peak power of CFOC channel is less than 8dBm.

Successful isolation of Helicobacter pylori after prolonged incubation from a patient with failed eradication therapy.

Helicobacter pylori (H pylori), a gastric pathogen, is a major cause of chronic gastritis and peptic ulcer disease, and is an important risk factor for the development of gastric malignancies. Culture of the bacterium from gastric biopsy is essential for the determination of drug resistance of H pylori. However, the isolation rates of H pylori from infected individuals vary from 23.

[Detection and identification of mycobacterial gene in skin lesions and lymph nodes in patients with sarcoidosis].

Objective: To investigate the possible role of mycobacteria in the pathogenesis of sarcoidosis.

Methods: We used nested polymerase chain reaction-restriction fragment length polymorphism (nPCR-RFLP) and gene sequencing method to examine 31 formalin-fixed, paraffin-embedded specimens (including 19 skin lesions and 12 lymph nodes ) obtained from patients with sarcoidosis, 14 normal skin specimens, and 3 cutaneous tuberculosis specimens.

Results: The 65kD mycobacterial heat shock protein gene was found in 4 out of 19 (21.

[Screening of serum protein biomarkers by surface-enhanced laser desorption and ionization with time-of-flight detection mass spectrometry in patients with mycosis fungoides].

Objective: To screen serum biomarkers in patients with mycosis fungoides (MF) using surface-enhanced laser desorption and ionization with time-of-flight detection mass spectrometry (SELDI-TOF-MS) technique.

Methods: Serum was analyzed from 14 patients with MF and 17 controls using CM10 Protein-chip to capture serum proteins, followed by Biomarker Wizard software analysis.

Results: In all specimens, about 131 protein peaks could be detected when the relative molecular weight ranged from 0 to 50 000.

Discovery and identification of potential biomarkers of pediatric acute lymphoblastic leukemia.

Background: Acute lymphoblastic leukemia (ALL) is a common form of cancer in children. Currently, bone marrow biopsy is used for diagnosis. Noninvasive biomarkers for the early diagnosis of pediatric ALL are urgently needed.

Psoriasis genome-wide association study identifies susceptibility variants within LCE gene cluster at 1q21.

We report the first large genome-wide association study (GWAS) in a Chinese population to identify susceptibility variants for psoriasis using a two-stage case-control design. In the first stage, we carried out a genome-wide association analysis in 1,139 cases and 1,132 controls of Chinese Han ancestry using Illumina Human 610-Quad BeadChips. In the second stage, we took top SNPs forward for replication in two independent samples of 5,182 cases and 6,516 controls of Chinese Han ancestry, and 539 cases and 824 controls of Chinese Uygur ancestry.

Genetic analysis of group A streptococcus isolates recovered during acute glomerulonephritis outbreaks in Guizhou Province of China.

In this study, 68 group A streptococcus (GAS) isolates associated with two outbreaks of acute glomerulonephritis (AGN) in China were analyzed by emm typing. A total of 11 different emm types were identified. Analysis of emm type distribution suggested that AGN outbreaks in two counties were caused by emm60.

[The effects of H. pylori and its crude extracted proteins on isolated rabbit parietal cells acid secretion].

Objective: To explore the effects of H. pylori and crude extracted proteins secreted by H. pylori (broth culture filtrate protein, BCF-P) on acid secretion from isolated rabbit parietal cells.

A novel DNA microarray for rapid diagnosis of enteropathogenic bacteria in stool specimens of patients with diarrhea.

A microarray technique for the detection and identification of enteropathogenic bacteria at the species and subspecies levels was developed in this study, and the target bacteria included pathogenic Escherichia coli, Vibrio cholerae, Vibrio parahaemolyticus, Salmonella enterica, Campylobacter jejuni, Shigellae, Yersinia enterocolitica, and Listeria monocytogenes. The virulence gene of each pathogen was chosen as the amplification target, labeled with a fluorescence dye by multiplex polymerase chain reaction (PCR), and hybridized to the specific virulence gene probes that had been immobilized on a microchip. Stool specimens from 34 patients with diarrhea were tested in this study.

Proteomic profiling of hepatitis B virus-related hepatocellular carcinoma in China: a SELDI-TOF-MS study.

Hepatocellular carcinoma (HCC) is one of the most common malignancies with high mortality, but its underlying molecular mechanisms remain not well understood. High-throughput, proteomic techniques targeting unique biological molecules may provide novel insights into HCC pathogenesis and prognosis. In this study, we systemically investigated tissue biomarkers of HCC by using surface-enhanced laser desorption and ionization time-of-flight mass spectrometry (SELDI-TOF-MS) technique.