Protection of laryngeal mucosa and function in laryngeal burns by heat absorption of perilaryngeal tissue.

Objective: The laryngeal tissue carries most of the heat during inhalation injury. This study aims to explore the heat transfer process and the severity of injury inside laryngeal tissue by horizontally studying the temperature rise process at various anatomical layers of the larynx and observing the thermal damage in various parts of the upper respiratory tract.

Methods: The 12 healthy adult beagles were randomly divided into four groups, and inhaled room temperature air (control group), dry hot air of 80 °C (group I), 160 °C (group II), and 320 °C (group III) for 20 min, respectively.

Epidemiology and risk prediction of patients with severe burns admitted to a burn intensive care unit in a burn center in beijing: A 5-year retrospective study.

Objective: This study was performed to describe the epidemiology of patients with severe burns hospitalized in a burn intensive care unit (BICU), explore the risk factors associated with the patients' outcomes and evaluate the ability of prognostic scoring systems as risk prediction of mortality.

Methods: The data for this study were derived from patients with severe burns in the BICU of Beijing Jishuitan Hospital from 2015 to 2019. The following epidemiological information and outcomes were collected for retrospective analysis: sex, age, date of injury, etiology of burn, admission time after injury, extent of burn, inhalation injury, length of stay, and outcome.

The nucleolus functions as the compartment for histone H2B protein degradation.

Histones are main components of chromatin, and the protein levels of histones significantly affect chromatin assembly. However, how histone protein levels are regulated, especially whether and how histones are degraded, is largely unclear. Here, we found that histone H2B is mainly degraded through the proteasome-mediated pathway, and the lysine-120 site of H2B is essential for its K48-linked polyubiquitination and degradation.

Epigenetic regulation of ferroptosis by H2B monoubiquitination and p53.

Monoubiquitination of histone H2B on lysine 120 (H2Bub1) is an epigenetic mark generally associated with transcriptional activation, yet the global functions of H2Bub1 remain poorly understood. Ferroptosis is a form of non-apoptotic cell death characterized by the iron-dependent overproduction of lipid hydroperoxides, which can be inhibited by the antioxidant activity of the solute carrier family member 11 (SLC7A11/xCT), a component of the cystine/glutamate antiporter. Whether nuclear events participate in the regulation of ferroptosis is largely unknown.

Mucosal fluid evaporation is not the method of heat dissipation from fourth-degree laryngopharyngeal burns.

This study was designed to explore whether mucosal fluid evaporation represents a method of heat dissipation from thermal air inhalation injury and to assess laryngopharyngeal tissue damage according to heat quantity changes of dry air and vapour. Fifteen adult male beagles were divided into five groups to inhale heated air or vapour for 10 min as follows: control group (ordinary air), group I (91-110 °C heated air), group II (148-175 °C heated air), group III (209-227 °C heated air), and group IV (96 °C saturated vapour). The heat quantity changes of the dry air and vapour were calculated via thermodynamic formulas.

Downregulation of LSD1 suppresses the proliferation, tumorigenicity and invasion of papillary thyroid carcinoma K1 cells.

The present study aimed to evaluate the effects of lysine-specific demethylase 1 (LSD1) downregulation, induced by small interfering RNA (siRNA) transfection, on the proliferation, colony formation, migration and invasion of the papillary thyroid carcinoma K1 cell line. The siRNA targeting LSD1 and scrambled non-targeting siRNA were each transfected into papillary thyroid carcinoma K1 cells. Downregulation of LSD1 mRNA and protein level was evaluated by reverse transcription-quantitative polymerase chain reaction, and immunocytochemical (ICC) analysis and western blotting, respectively.

siRNA targeting RBP2 inhibits expression, proliferation, tumorigenicity and invasion in thyroid carcinoma cells.

In order to estimate the effects of small interfering RNA (siRNA) targeting retinoblastoma binding protein 2 (RBP2) on the proliferation, expression, invasion, migration and tumorigenicity abilities of papillary thyroid carcinoma K1 cells, siRNA targeting RBP2 (RBP2-siRNA) and negative control siRNA were transfected into K1 cells. The mRNA levels of RBP2 in the transfected cells were estimated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and the protein levels of RBP2 in these cells were evaluated by western blot analysis and immunocytochemical (ICC) analyses. The growth, tumorigenicity, migration and invasion abilities of the transfected cells were measured by Cell Counting Kit-8 (CCK-8), soft agar colony formation and transwell chamber assay, respectively.

PLCE1 gene in esophageal cancer and interaction with environmental factors.

Objective: To study the PLCE1 gene rs2274223 polymorphism with regard to esophageal cancer and its interaction with diet, lifestyle, psychological and environmental factors in Southwest Shandong province.

Materials And Methods: A case series study (case-case) was conducted. Questionnaire data were collected and 3 ml-5 ml venous blood was drawn for DNA extraction among the qualified research subjects.

Laryngeal morphologic changes and epidemiology in patients with inhalation injury: a retrospective study.

Background And Objectives: Laryngeal morphologic changes are important in risk assessment of upper airway obstruction (UAO) after inhalation injury. This retrospective study evaluates the clinical application of laryngeal burn classification system.

Materials And Methods: Clinical data from January 1999 to June 2013 were analyzed retrospectively.

Short low concentration cisplatin treatment leads to an epithelial mesenchymal transition-like response in DU145 prostate cancer cells.

Background: Prostate cancer is one of the main causes of cancer death, and drug resistance is the leading reason for therapy failure. However, how this occurs is largely unknown. We therrfore aimed to study the response of DU145 cells to cisplatin.

[Pathological changes in different parts of the larynx in canines following laryngeal burns induced by inhalation of hot air at various temperatures].

Objective: To examine pathological changes in different parts of the larynx in canines after laryngeal burns induced by hot dry air at various temperatures and compare the pathological results with clinical typing.

Methods: Eighteen healthy, male, adult dogs were randomly assigned to inhale hot dry air at 80 °C (Group 1), 160 °C (Group 2) or 320 °C (Group 3) for 20 min to induce inhalation injury. Pathological changes after hot air inhalation were examined at different locations of the larynx including the epiglottis, laryngeal vestibule, vocal folds and trachea.

Pathological changes of the three clinical types of laryngeal burns based on a canine model.

Objective: The study was designed to examine pathological changes of inhalational laryngeal burns of three clinical types: congestive, oedematous and obstructive.

Methods: A total of 18 healthy, male, adult Beagle dogs were randomly assigned to inhale hot dry air at room temperature (group C), 80°C (Group 1), 160°C (Group 2) or 320°C (Group 3) for 20min to induce inhalation injury. Each larynx was evaluated and scored based on the 'clinical scoring and typing system of laryngeal burns at early stage'.

Firework injuries at a major trauma and burn center: A five-year prospective study.

Background And Objective: In China, fireworks are an integral part of the celebration of the annual Spring Festival, but the number of injuries associated with their private use seen in emergency rooms increases dramatically. To raise awareness and help guide future prevention practices in this city, we investigated the epidemiology of firework-related injuries presented at our trauma and burn center in Beijing during the Spring Festivals of 2007-2011.

Methods: Patients were interviewed using a pre-coded questionnaire to elicit information regarding age, gender, causes, injured body part, type of injury, diagnosis, and disposition.

Circulational heat dissipation of upper airway: Canine model of inhalational thermal injury.

Background: The upper airway has powerful heat-absorbing ability, in which the blood circulation may play an important role.

Objective: This study aimed to explore the circulational heat-dissipating ability, and to investigate the contribution of blood circulation to the heat-absorbing ability of upper airway.

Methods: 18 adult, male Beagle dogs were divided into three groups to inhale thermal dry air of 70-80°C, 150-160°C or 310-320°C for 20min.

Clinicopathological implications of leptin and leptin receptor expression in papillary thyroid cancer.

The role of leptin and its receptors (OBRs) in the pathogenesis of various primary human malignancies has been demonstrated. However, their expression and clinicopathological significance in papillary thyroid cancer (PTC) is not fully understood. In this study, we examined the expression of leptin and OBRs in 76 PTC samples using immunohistochemistry, and their associations with clinicopathological parameters were evaluated.

[Repair of circumferential wound in the wrist region due to high-voltage electrical burn using combined abdominal axial pattern flaps].

Objective: To explore the method for repairing circumferential wound in the wrist region due to high-voltage electrical burn.

Methods: Six patients with circumferential wound in the wrist region after high-voltage electrical burn were admitted to our hospital from January 2009 to December 2011. After debridement, wounds in the wrist were repaired with combined abdominal axial pattern flaps.

[Skin irritation and sensitization of swine acellular dermal matrix treated with hyaluronic acid].

Objective: To evaluate the skin irritation and sensitization potential of the swine acellular dermal matrix treated with hyaluronic acid (SADM-HA).

Methods: (1) Skin irritation test. Twelve New Zealand rabbits were divided into SADM-HA group, allogeneic skin group, and (human) xeno-skin group according to the random number table, with 4 rabbits in each group.

[Repair of deep burn wound of neck with supraclavicular island flap].

Objective: To observe the therapeutic effect of supraclavicular island flap in repairing deep burn wound of neck.

Methods: Six patients with deep burn of neck hospitalized from January 2009 to June 2011 were enrolled in the study. Their total burn area ranged from 6% to 22% TBSA, of which full-thickness area ranged from 3% to 22% TBSA.

[Lay emphasis on the aesthetic effect of wound repair].

"Survival first" has been the leading rule in burn surgery for a long time. However, the life quality of patients in future should also be emphasized on the strategic level during the early treatment of patients with massive burn. Aesthetics is also extremely important in wound repair, and it should form a clear conception in the mind of the attending surgeons.

Measuring surface temperature and grading pathological changes of airway tissue in a canine model of inhalational thermal injury.

Background: Airway tissue shows unexpected invulnerability to heated air. The mechanisms of this phenomenon are open to debate.

Objective: This study was designed to measure the surface temperatures at different locations of the airway, and to explore the relationship between the tissue's surface temperature and injury severity.

[Treatment of full-thickness electric burn of skull combined with cerebral contusion and intracranial infection].

This article reports the treatment of a patient suffering from full-thickness electric burn of skull combined with cerebral contusion and intracranial infection to provide experience in treating such patients. Based on detailed analysis on patient's condition and CT results, several operations of surgery and anti-infection treatment were performed on the patient. The wounds healed 6 weeks after injury.

Epidemiology of 1974 burn patients at a major burn center in Beijing: a nine-year study.

To date, little epidemiological data are available on burns in China. This study describes the characteristics of burn patients admitted to a major burn center in Beijing to show trends in admission and outcomes in burned patients to share information about the current state of care for burned patients in our burn center. A retrospective study on 1974 burn patients admitted to Jishuitan Hospital in Beijing was conducted during the 9-year period from 2000 to 2008, and data were collected on age, gender, TBSA, etiology, length of hospital stay, mortality, and inhalation injury.

Temperature distribution in the upper airway after inhalation injury.

Objective: The aim of the study was to establish an animal model of laryngeal burn and to investigate the temperature distribution of heated air in the upper airway.

Methods: The animal model was established by inhalation of dry heated air at 80, 160 and 320 °C in 18 healthy, male, adult hybrid dogs. Time for inducing injury was set at 20 min.

[Early treatment of high-voltage electric burn wound in the limbs].

Objective: To summarize the experience of early treatment of high-voltage electric burn wounds in the limbs.

Methods: Fifty-four patients (50 males and 4 females, aged from 10 to 56 years) with high-voltage electric burn wounds in 97 limbs (67 upper limbs and 30 lower limbs) were hospitalized in our burn wards from January 2003 to December 2010. A total of 119 burn wounds in wrist-forearm, forearm-elbow-upper arm, shoulder-axillary region, ankle-foot, lower leg, around the knee, thigh-inguinal region were treated with incision for decompression within 10 days after burn.