Publications by authors named "Zhang Guimin"

Halophilic proteins possess unique structural properties and show high stability under extreme conditions. This distinct characteristic makes them invaluable for application in various aspects such as bioenergy, pharmaceuticals, environmental clean-up, and energy production. Generally, halophilic proteins are discovered and characterized through labor-intensive and time-consuming wet lab experiments.

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  • The study examines olfactory dysfunction following head trauma, highlighting challenges in diagnosis due to injuries in olfactory brain areas and the lack of standardized imaging criteria.
  • Researchers compared subjective olfactory function and brain volume (gray-matter and white-matter) between 56 patients with traumatic olfactory dysfunction and 45 patients with other causes of olfactory issues.
  • Results showed that the trauma group scored significantly lower on olfactory tests, indicating more profound dysfunction compared to the control group, with the findings suggesting a need for improved diagnostic criteria for traumatic olfactory disorders.
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As a type of transdermal drug delivery system (TDDS), Microneedles (MNs) have garnered significant attention from researchers due to their ability to penetrate the stratum corneum (SC) of the skin, enhance drug permeability and bioavailability, avoid first-pass metabolism, and cause minimal damage to the skin. This makes them particularly suitable for localized transdermal drug delivery. Dissolvable microneedles (DMNs) can encapsulate sensitive particles, provide high drug-loading capacity, and possess biodegradability and biocompatibility, attracting extensive research interest.

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Background: Triphala, is a composite of three individual botanical drugs: , , and . It exhibits properties such as heatclearing, anti-inflammatory, anti-fatigue, antioxidant, and antibacterial effects,making it extensively utilized in India and Tibet. It has been found to exhibitinhibitory effects on (); however, further comprehensive research is still needed to elucidate its specific antibacterial mechanism.

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Astragalus membranaceus has been used in traditional Chinese medicine for over 2,000 years. Its major active triterpenoid saponins, astragalosides, have attracted great attention due to their multiple health benefits and applications in medicine. Despite this, the biosynthetic machinery for astragalosides remains enigmatic.

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Background: Retz, known as the King of Tibet, is considered a functional food in China, celebrated for its antioxidant, immune-modulating, antibacterial, and anti-inflammatory properties. Chebulinic acid, derived from aqueous extracts of Retz, is known for its anti-inflammatory properties. However, its potential as an anti- (HP) agent has not been fully explored.

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  • Slow transit constipation (STC) is becoming more common, and Shouhui Tongbian capsule (SHTB), a traditional Chinese medicine, shows promise in treating it, although its mechanisms are not fully understood.
  • Researchers used various methods, like mass spectrometry and microbiome analysis, to study how SHTB works in repairing gut health and affecting lipid metabolism in STC.
  • Results showed SHTB improved intestinal barrier function, decreased inflammation, and normalized gut bacteria, highlighting its role in reducing lipid buildup and restoring microbial balance as key treatment mechanisms for STC.*
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  • * A new method involving metabolomics techniques like UPLC-MS/MS and DESI-MSI was used to analyze metabolic changes in STC rats, revealing that SHTB treatment normalized abnormal bile acid levels and reduced intestinal injury by increasing muscle layer thickness.
  • * SHTB treatment inhibited the excessive reabsorption of bile acids in the ileum by affecting specific transporters and significantly increased the presence of beneficial bile acids in the colon tissue of STC
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Ethnopharmacological Relevance: Cerebral ischemic stroke (CIS) is one of the most important factors leading to death and disability, which seriously threaten the survival and health of patients. The intentional flora and its derived metabolites are demonstrated to play vital roles in the physiology and onset of CIS. Shouhui Tongbian Capsules (SHTB), a Traditional Chinese Medicine, could regulate gut microbiota and metabolites.

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  • The study aimed to evaluate a new method to prevent the Argentinian flag sign during anterior capsulotomy in patients with intumescent cataracts, with proper registration and documentation.
  • A total of 61 patients underwent several preoperative examinations, and the new technique involved using an air bubble and puncturing to manage the liquefied cortex, ensuring no Argentinian flag sign occurred during the procedure.
  • Results showed successful capsulorrhexis and satisfactory postoperative outcomes for all patients, with minimal complications, indicating the technique is simple, convenient, and worth clinical promotion.
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  • Gastric cancer is a major global health issue, and this study explores the impact of Helicobacter pylori (HP) infection on gastric cancer cells, focusing on their viability and migration.
  • The research involved multiple assays to assess the effects of HP on cancer cell behaviors and used bioinformatics to identify genes associated with HP infection, with particular attention to their implications for immunity and drug sensitivity.
  • Results indicated that while HP infection doesn't significantly affect cell viability, it enhances the migration of gastric cancer cells; 1405 genes related to HP infection were identified, and seven key genes were validated that appear to influence migration and treatment response in gastric cancer.
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Ulcerative colitis (UC) is a chronic, relapsing nonspecific intestinal inflammatory disease. It is difficult for a single drug to treat UC effectively and maintain long-term efficacy. There is an urgent need to find new drugs and treatment strategies.

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Purpose: A growing body of evidence has elucidated that the gut microbiota has a crucial impact on the pathophysiological process of atopic diseases. Eosinophilic chronic rhinosinusitis with nasal polyps (eCRSwNP) is a local atopic disease of the systemic immune response. Alterations in the gut microbiome in eCRSwNP patients remain largely undefined.

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The mutual interactions of endoplasmic reticulum (ER) resident proteins in the ER maintain its functions, prompting the protein folding, modification, and transportation. Here, a new method, named YST-PPI (YESS-based Split fast TEV protease system for Protein-Protein Interaction) was developed, targeting the characterization of protein interactions in ER. YST-PPI method integrated the YESS system, split-TEV technology, and endoplasmic reticulum retention signal peptide (ERS) to provide an effective strategy for studying ER in situ PPIs in a fast and quantitative manner.

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  • - Zearalenone (ZEN) is a harmful fungal toxin linked to reproductive issues in both animals and humans, and its removal is crucial for health and safety.
  • - Current methods using ZEN lactonases struggle with thermal stability, but a new engineered enzyme variant, I160Y-G242S, shows about 40% activity retention at 55℃ after 10 minutes, making it the most heat-tolerant ZEN hydrolase reported.
  • - The I160Y-G242S variant also exhibits twice the effectiveness compared to its predecessor, thanks to enhanced stability and improved interactions with its substrate, making it a promising option for ZEN degradation in industrial applications.
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  • Hypertrophic cardiomyopathy (HCM) is a hereditary condition characterized by thickening of the heart muscle, contributing significantly to cardiac-related deaths, with ongoing research exploring the role of immune inflammation in its development.
  • Five machine learning algorithms were used to identify five new biomarkers (DARS2, GATM, MGST1, SDSL, ARG2) linked to HCM, with GATM and MGST1 showing strong diagnostic potential based on their elevated expression in HCM tissues compared to normal tissues.
  • A new risk assessment model utilizing GATM and MGST1 was developed and validated, demonstrating effective performance in distinguishing HCM patients, while also indicating these markers have prognostic relevance in both HCM and various cancers.
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The Publisher regrets that this article is an accidental duplication of an article that has already been published, http://dx.doi.org/10.

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Staphylococcus aureus is a gram-positive bacteria, and its virulence factors can cause many kinds of infections, such as pneumonia, sepsis, enteritis and osteomyelitis. Traditional antibiotics can not only kill bacteria, but also easily lead to bacterial resistance. Jingfang Mixture (JFM) has the effects of inducing sweating and relieving the exterior, dispelling wind and eliminating dampness, and is commonly used in clinic to prevent and treat epidemic diseases and infectious diseases.

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Ethnopharmacological Relevance: Mailuo Shutong Pill (MLST), a traditional Chinese medicine (TCM), has been widely used for clearing heat and detoxifying, eliminating stasis and dredging meridians, dispelling dampness and diminishing swelling. Earlier study found that MLST could improve cerebral ischemic-reperfusion injury, however, the potential mechanism has not been well evaluated.

Aim Of Study: In this study, a well established and widely used mice model of middle cerebral artery occlusion/reperfusion (MCAO/R) was preformed to evaluate the protective function of MLST on cerebral ischemic-reperfusion injury and further discuss the potential pharmacological mechanisms.

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Natural products-based screening of active ingredients and their interactions with target proteins is an important ways to discover new drugs. Assessing the binding capacity of target proteins, particularly when multiple components are involved, presents a significant challenge for sensors. As far as we know, there is currently no sensor that can accomplish high-throughput quantitative analysis of natural product-target protein binding capacity based on Raman spectroscopy.

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The Food and Drug Administration (FDA) recently reported a new nitrosamine impurity in sitagliptin that was named nitroso-STG-19 (NTTP), whose acceptable intake limit was extremely low at 37 ng per day. In addition, NTTP was found to be a degradation impurity in sitagliptin tablets, which was formed by the reaction of 3-(trifluoromethyl)-5,6,7,8-tetrahydro-[1,2,4]triazolo[4,3-]pyrazine hydrochloride and nitrite salts introduced excipients. Consequently, the NTTP content in tablets was larger than that in active pharmaceutical ingredients (APIs).

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Background: The aqueous extract of the dried buds of Syzygium aromaticum (SAAE) have the potential to alleviate Helicobacter pylori infection, but the specific molecular mechanism has not been fully elucidated.

Purpose: This study aimed to investigate the underlying mechanisms of SAAE on H. pylori pathogenicity.

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Intracerebral hemorrhage (ICH) can induce intensive oxidative stress, neuroinflammation, and brain cell apoptosis. However, conventional methods for ICH treatment have many disadvantages. There is an urgent need for alternative, effective therapies with minimal side effects.

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Background: Trace levels of organic and inorganic lead and mercury species in the environment, including divalent lead (Pb), trimethyllead (TML), divalent mercury (Hg), monomethylmercury (MeHg), and ethylmercury (EtHg), are highly toxic to humans and ecology. It is of great importance for speciation of lead and mercury to evaluate the toxicity of lead and mercury and their biogeochemistry in the environment. However, simultaneous multi-elemental enrichment and speciation at trace level remains a challenge.

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