MDM2 interacts with PTEN to inhibit endothelial cell development and promote deep vein thrombosis via the JAK/STAT signaling pathway.

Deep vein thrombosis (DVT) is a prevalent clinical condition, which markedly affects patients' quality of life, commonly leading to post‑thrombotic syndrome. The present study aimed to elucidate the intricate interplay between murine double minute‑2 (MDM2) and phosphatase and tensin homolog (PTEN), thus shedding new light on their role in the pathogenesis of DVT. The results showed that both MDM2 and PTEN were upregulated in venous blood samples obtained from patients with DVT.

Two Novel and Two Recurrent Variants of the ADAR1 Gene in Three Chinese Families with Dyschromatosis Symmetrica Hereditaria.

Purpose: Dyschromatosis symmetrica hereditaria (DSH) is a rare autosomal dominant inherited pigmentary dermatosis. The gene responsible for DSH has been identified as adenosine deaminase acting on RNA1 (). This study aimed to identify the causative variants in the gene in three Chinese families with DSH.

Re-evaluating the necessity of routine laboratory monitoring during isotretinoin therapy for acne.

In this letter, we discuss the topic of necessity of routine laboratory monitoring during isotretinoin treatment for acne. According to Park and colleagues, it is advisable to monitor the levels of triglycerides, alanine aminotransferase, and aspartate aminotransferase every 5 to 6 months. Additionally, the levels of total cholesterol and low-density lipoprotein should be checked within the first two months of treatment.

A spectral bias-error stepwise correction method of plasma image-spectrum fusion based on deep learning for improving the performance of LIBS.

Poor spectral stability seriously hinders the wide application of laser-induced breakdown spectroscopy (LIBS), so how to improve its stability is the focus, hotspot, and difficulty of current research. In this study, to achieve high precision quantitative analysis under complex detection conditions, utilizing the fusion of multi-dimensional plasma information and the integration of physical models and algorithmic models, a spectral bias-error stepwise correction method of plasma image-spectrum fusion based on deep learning (SBESC-PISF) was proposed. In this method, based on the statistical properties of LIBS spectra, the actual obtained spectra were decomposed into three parts: the ideal spectral intensity related only to the element concentration, and the spectral bias and spectral error caused by the fluctuation of complex high-dimensional plasma parameters.

Artificial intelligence applications in the diagnosis and treatment of bacterial infections.

The diagnosis and treatment of bacterial infections in the medical and public health field in the 21st century remain significantly challenging. Artificial Intelligence (AI) has emerged as a powerful new tool in diagnosing and treating bacterial infections. AI is rapidly revolutionizing epidemiological studies of infectious diseases, providing effective early warning, prevention, and control of outbreaks.

Stereotactic radiotherapy vs whole brain radiation therapy in EGFR mutated NSCLC: Results & reflections from the prematurely closed phase III HYBRID trial.

Background: All known randomized trials of stereotactic radiotherapy (SRT) versus whole brain radiotherapy (WBRT) for brain metastases (BMs) comprise mixed histologies. The phase III HYBRID trial (NCT02882984) attempted to evaluate the non-inferiority of SRT vs. WBRT specifically for EGFR-mutated non-small cell lung cancer (EGFRm NSCLC) BMs.

Two B-box proteins orchestrate vegetative and reproductive growth in summer chrysanthemum.

Floral transition, the switch from vegetative to reproductive growth, is extremely important for the growth and development of flowering plants. In the summer chrysanthemum, CmBBX8, a member of the subgroup II B-box (BBX) family, positively regulates the transition by physically interacting with CmERF3 to inhibit CmFTL1 expression. In this study, we show that CmBBX5, a B-box subgroup I member comprising two B-boxes and a CCT domain, interacts with CmBBX8.

Association of cardiovascular disease prevalence with BMD and fracture in men with T2DM.

Background: Patients with type 2 diabetes mellitus (T2DM) are predisposed to cardiovascular disease (CVD). Bone mineral density (BMD) is linked to CVD, but most studies focused on women. Our analysis aims to explore the association of BMD and fracture with the prevalence of CVD in men with T2DM.

Plasma parameters correction method based on plasma image-spectrum fusion for matrix effect elimination in LIBS.

Matrix effect is one of the obstacles that hinders the rapid development of laser-induced breakdown spectroscopy (LIBS), and it is currently a hot, challenging, and focal point in research. To eliminate the matrix effect, this study proposed a plasma parameters correction method based on plasma image-spectrum fusion (PPC-PISF). This method corrects the total number density, plasma temperature, and electron number density variations caused by matrix effect using effective features in plasma images and spectra.

UHPLC-qTOF-MS-Based Nontargeted Metabolomics to Characterize the Effects of Capsaicin on Plasma and Skin Metabolic Profiles of C57BL/6 Mice-An In vivo Experimental Study.

Background: Capsaicin is the main compound found in chili pepper and has complex pharmacologic effects. This study aimed to elucidate the mechanism of the effect of capsaicin on physiological processes by analyzing changes in metabolites and metabolic pathways.

Methods: Female C57BL/6 mice were divided into two groups(n = 10/group) and fed with capsaicin-soybean oil solution(group T) or soybean oil(group C) for 6 weeks.

Two Novel and Three Recurrent Mutations in the Mevalonate Pathway Genes in Chinese Patients with Porokeratosis.

Purpose: Porokeratosis (PK) is a chronic autosomal-dominant cutaneous keratinization disorder exhibiting clinical and genetic heterogeneity. Mevalonate decarboxylase (), farnesyl diphosphate synthase (), phosphomevalonate kinase(), and mevalonate kinase genes(), which encode the mevalonate pathway, are disease-causing genes in PK.

Patients And Methods: Data and blood samples were collected from two Chinese families and five sporadic patients with porokeratosis.

Acetyltransferases CBP/p300 Control Transcriptional Switch of β-Catenin and Stat1 Promoting Osteoblast Differentiation.

CREB-binding protein (CBP) (CREBBP) and p300 (EP300) are multifunctional histone acetyltransferases (HATs) with extensive homology. Germline mutations of CBP or p300 cause skeletal abnormalities in humans and mice. However, the precise roles of CBP/p300 in bone homeostasis remain elusive.

USP1 promotes cholangiocarcinoma progression by deubiquitinating PARP1 to prevent its proteasomal degradation.

Despite its involvement in various cancers, the function of the deubiquitinase USP1 (ubiquitin-specific protease 1) is unexplored in cholangiocarcinoma (CCA). In this study, we provide evidence that USP1 promotes CCA progression through the stabilization of Poly (ADP-ribose) polymerase 1 (PARP1), consistent with the observation that both USP1 and PARP1 are upregulated in human CCA. Proteomics and ubiquitylome analysis of USP1-overexpressing CCA cells nominated PARP1 as a top USP1 substrate.

Complex-valued neural-operator-assisted soliton identification.

The numerical determination of solitary states is an important topic for such research areas as Bose-Einstein condensates, nonlinear optics, plasma physics, and so on. In this paper, we propose a data-driven approach for identifying solitons based on dynamical solutions of real-time differential equations. Our approach combines a machine-learning architecture called the complex-valued neural operator (CNO) with an energy-restricted gradient optimization.

Visualization of laser-induced breakdown spectroscopy data of mouse organs based on the feature extraction method.

At present, there is no comprehensive and systematic research on laser-induced breakdown spectroscopy (LIBS) data visualization. In particular, the LIBS spectra of biological samples have large noise and weak signals, which seriously affect the feature visualization. Here, three commonly used sample visualization methods were compared, and a new method was applied for tissue sample visualization.

Untargeted Plasma Lipidomics Reveal Perturbed Metabolites of Glycerophospholipids, and Sphingolipids in Moderate-to-Severe Acne.

Background: Acne vulgaris (AV) is a common inflammatory disorder involving the pilosebaceous unit. The study aimed to explore the plasma lipidome signatures and identify specific lipid biomarkers in moderate-to-severe acne patients.

Patients And Methods: Untargeted plasma lipidomic analysis using ultra-high performance liquid chromatography system (UHPLC) coupled to q-extraction plus was employed on 30 moderate-to-severe acne patients aged between 16-25 years and 30 healthy controls.

Liquid tumor microenvironment enhances WNT signaling pathway of peritoneal metastasis of gastric cancer.

Gastric cancer remains one of the most prevalent tumors worldwide and peritoneal metastasis is responsible for approximately 60% of death in advanced gastric cancer patients. However, the underlying mechanism of peritoneal metastasis is poorly understood. We have established organoids derived from malignant ascites (MA) of gastric cancer patients and noticed that MA supernatant could strongly increase the colony formation of organoids.

Using urban building energy modeling to quantify the energy performance of residential buildings under climate change.

The building sector is facing a challenge in achieving carbon neutrality due to climate change and urbanization. Urban building energy modeling (UBEM) is an effective method to understand the energy use of building stocks at an urban scale and evaluate retrofit scenarios against future weather variations, supporting the implementation of carbon emission reduction policies. Currently, most studies focus on the energy performance of archetype buildings under climate change, which is hard to obtain refined results for individual buildings when scaling up to an urban area.

Two Novel and a Recurrent Mutations in Chinese Population with Hailey-Hailey Disease.

Purpose: Hailey-Hailey disease (HHD), also known as familial benign chronic pemphigus, is a rare autosomal dominant inherited blistering dermatosis. Pathogenic variants in have been associated with HHD since 2000. This study aimed to identify the mutations in the gene in two Chinese pedigrees and two sporadic cases with HHD.

Nerve conduction velocity is independently associated with bone mineral density in type 2 diabetes mellitus.

Aim: This study investigated the association between nerve conduction velocity (NCV) and bone mineral density (BMD) in patients with type 2 diabetes mellitus (T2DM).

Methods: This study retrospectively collected medical data of T2DM patients who underwent dual-energy X-ray absorptiometry and nerve conduction study at the Shanghai Ruijin Hospital, Shanghai, China. The primary outcome was the total hip BMD T-score.