METTL3 mediated WISP1 mA modification promotes epithelial-mesenchymal transition and tumorigenesis in laryngeal squamous cell carcinoma via mA reader IGF2BP1.

Object: N6-methyladenosine (mA), is well known as the most abundant epigenetic modification in messenger RNA, but its influence on laryngeal squamous cell carcinoma (LSCC) remains largely unexplored and poorly understood. This study was designed to explore the effects of mA on WISP1-mediated epithelial-mesenchymal transition (EMT) and tumorigenesis in LSCC.

Methods: mA methylated and expression levels of WISP1 in LSCC tumor tissues and cells were measured by MeRIP-qPCR, qRT-PCR, and western blotting.

Emergence of Decapod iridescent virus 1 in cultured shrimp from Taiwan in 2020.

Objectives: This study was to identify and characterize Decapod iridescent virus 1 (DIV1) in the outbreaks reported in two whiteleg shrimp farms and one black tiger shrimp farm located in northern Taiwan in 2020.

Methods: The histopathology, electron microscopy and polymerase chain reaction (PCR) specific for the DIV1 were used to identify the virus, and the phylogenetic analysis was performed by comparing the major capsid protein gene fragment of DIV1s from Taiwan with reference sequences of the family Iridoviridae.

Results: DIV1 was identified by diagnostic PCR and caused mild mortality (20%) in cultured Penaeus monodon and high mortality (100%) in cultured whiteleg shrimp.

Resection of the intracavernous sinus tumors using a purely endoscopic endonasal approach.

Resection of the cavernous sinus (CS) lesions has been a surgical challenge because the anatomy of the CS presents a high grade of complexity. This report describes the feasibility of the purely endoscopic endonasal approach to the CS. Twenty-five patients with intracavernous sinus tumors were treated with a purely endoscopic endonasal approach.

Transoral endoscopic odontoidectomy to decompress the cervicomedullary junction.

Study Design: Clinical study.

Objective: To investigate the feasibility of the transoral endoscopic odontoidectomy without occipitocervical fusion.

Summary Of Background Data: Endoscopic transnasal resection of the odontoid process is less invasive than the conventional transoral odontoidectomy.

Endoscopic endonasal management of trigeminal schwannomas extending into the infratemporal fossa.

Extracranial trigeminal schwannomas extending into the infratemporal fossa are rare. The traditional surgical approaches to the infratemporal fossa are associated with complications, such as facial nerve dysfunction, hearing loss, dental malocclusion and cosmetic problems. We report eight patients (four males, four females, age range=31-62 years) who were treated between 2004 and 2009 for extracranial trigeminal schwannomas extending into the infratemporal fossa.