Isoalcanivorax beigongshangi sp. nov., isolated from the fermented grains of Chinese baijiu.

A bacterial strain, REN37, was isolated from fermented grains of Baijiu samples collected from Sichuan, PR China. The cells of strain REN37 was Gram-negative and aerobic. The cellular morphology exhibited rod-shaped cells without flagellum, displaying non-motility.

The Potential of Microorganisms for the Control of Grape Downy Mildew-A Review.

(Berk.et Curtis) Berl. Et de Toni is the pathogen that causes grape downy mildew, which is an airborne disease that severely affects grape yield and causes huge economic losses.

Resuscitation Promotion Factor: A Pronounced Bacterial Cytokine in Propelling Bacterial Resuscitation.

While confronted with unfavorable growth conditions, bacteria may transform into the dormant state, such as viable but nonculturable (VBNC) state, which is a reversible state characterized by low metabolic activity and lack of division. These dormant cells can be reactivated through the influence of the resuscitation promoting factor (Rpf) family, which are classified as autocrine growth factors and possess peptidoglycan hydrolase activities. To date, with the significant resuscitation or growth promotion ability of Rpf, it has been extensively applied to increasing bacterial diversity and isolating functional microbial species.

sp. nov., isolated from baijiu fermented grains, could transform ginsenosides of .

A Gram-stain-positive actinomycete, designated REN17, was isolated from fermented grains of Baijiu collected from Sichuan, PR China. It exhibited branched substrate mycelia and a sparse aerial mycelium. The optimal growth conditions for REN17 were determined to be 28 °C and pH 7, with a NaCl concentration of 0 % (w/v).

Resuscitation of baijiu pit mud bacteria based on Rpf protein of Umezawaea beigongshangensis.

The pit mud in the Baijiu fermentation cellar is an abundant microbial resource that is closely related to the quality of baijiu. However, many naturally existing species might be in a viable but nonculturable (VBNC) state, posing challenges to the isolation and application of functional species. Herein, a previously isolated strain from baijiu mash, Umezawaea beigongshangensis, was found to contain the rpf gene that encodes resuscitation promotion factor (Rpf).

Cry1Ac Mixed with Gentamicin Influences the Intestinal Microbial Diversity and Community Composition of Pink Bollworms.

Pink bollworms severely affect the production of cotton. The method currently used for pink bollworm control is the planting of Bt () protein-expressing transgenic cotton. However, pink bollworms can develop strong resistance to Bt proteins in transgenic cotton because of the large planting area and long planting time of this crop, which severely affects the control of pink bollworms.

The Adenylate Cyclase-Encoding Gene Is Involved in Mycoparasitism.

is an excellent biocontrol fungus against numerous fungal plant pathogens. The cAMP signaling pathway is a crucial signal transduction pathway in fungi. To date, the role of the cAMP signaling pathway in mycoparasitism remains unknown.

Transcriptomic Response of Mycoparasitizing .

is an important mycoparasitism biocontrol agent that exhibits excellent control efficacy against numerous fungal plant pathogens. Transcriptomic sequencing may be used to preliminarily screen mycoparasitism-related genes of against fungal pathogens. The present study sequenced and analyzed the transcriptome of mycoparasitizing a Basidiomycota (phylum) fungal pathogen, , under three touch stages: the pre-touch stage, touch stage and after-touch stage.

The Effect of Buckwheat Resistant Starch on Intestinal Physiological Function.

Resistant starch appears to have promising effects on hypertension, cardiovascular and enteric illness. The influence of resistant starch on intestinal physiological function has drawn great attention. In this study, we first analyzed the physicochemical characteristics, including the crystalline properties, amylose content, and anti-digestibility among different types of buckwheat-resistant starch.

Development of Microbial Indicators in Ecological Systems.

Indicators can monitor ecological environment changes and help maintain ecological balance. Bioindicators are divided into animal, plant, and microbial indicators, of which animal and plant indicators have previously been the most researched, but microbial indicators have drawn attention recently owing to their high sensitivity to the environment and their potential for use in monitoring environmental changes. To date, reviews of studies of animals and plants as indicator species have frequently been conducted, but reviews of research on microorganisms as indicator species have been rare.

Monitoring Mycoparasitism of against Using GFP.

is an important mycoparasite, with great potential for controlling numerous plant fungal diseases. Understanding the mechanisms and modes of action will assist the development and application of this biocontrol fungus. In this study, the highly efficient 67-1 strain was marked with the green fluorescent protein (GFP), and the transformant possessed the same biological characteristics as the wild-type strain.

cAMP Signalling Pathway in Biocontrol Fungi.

Biocontrol is a complex process, in which a variety of physiological and biochemical characteristics are altered. The cAMP signalling pathway is an important signal transduction pathway in biocontrol fungi and consists of several key components. The G-protein system contains G-protein coupled receptors (GPCRs), heterotrimeric G-proteins, adenylate cyclase (AC), cAMP-dependent protein kinase (PKA), and downstream transcription factors (TFs).

Effects of mulching film on soil microbial diversity and community of cotton.

Different types of mulching film could variously influence soil properties and plant growth. Yet, surprisingly few studies have investigated the effects of mulching film upon soil microbial diversity and community structure. In this research, two kinds of mulching film, a traditional PE (polyethylene) mulching film and a degradable PBAT ((Poly [butyleneadipate-co-terephthalate])) mulching film, were applied to cotton (Gossypium spp.

Sporosarcina beigongshangi sp. nov., isolated from pit mud of Baijiu.

A Gram-positive, aerobic and short rod-shaped bacterium designated REN13, was isolated from pit mud of Baijiu, in Sichuan province, China. Strain REN13 could grow at 10-50 ℃, pH 6.0-9.

Umezawaea beigongshangensis sp. nov., Isolated From the Mash of Baijiu.

A Gram-positive, aerobic and non-motile actinobacterial strain, designated REN6, was isolated from the mash of Baijiu (Chinese spirits, a kind of distilling liquor is made by cooking, saccharification, fermentation and distillation) collected from Sichuan Province Region, China, and characterized using a polyphasic approach. Morphological and chemotaxonomic properties of strain REN6 were consistent with the description of the genus Umezawaea, such as the spore arrangement, the abundant aerial mycelium and the fragmented substrate mycelium. The diamino acid of peptidoglycan is meso-diaminopimelic acid.

Pseudoxanthomonas beigongshangi sp. nov., a novel bacteria with predicted nitrite and nitrate reduce ability isolated from pit mud of Baijiu.

A Gram-negative and rod-shape bacterium designated REN9, was isolated from pit mud of Baijiu in Sichuan, China. The 16S rRNA sequence of strain REN9 had a high similarity to Pseudoxanthomonas indica P15 (99.21%), P.

Brevibacterium renqingii sp. nov., isolated from the Daqu of Baijiu.

Two bacterial strains, designated REN4 and REN4-1, were isolated from daqu sample collected from baijiu factory located in Shanxi, China. The two strains shared highly similar 16S rRNA gene sequences (99.67% identities) and formed a monophyletic clade within the Brevibacterium 16S rRNA gene tree, showing 97.

Culturing Bacteria From Fermentation Pit Muds of Baijiu With Culturomics and Amplicon-Based Metagenomic Approaches.

The Baijiu-making microbiota has an important role in the alcohol production, flavor, and character of Baijiu. 16S rRNA gene sequencing revolutionized the understanding of Baijiu-making microbiota. In this study, nine phyla, 23 classes, 49 orders, 99 families, and 201 genera were detected in pit muds (PMs) by 16S rRNA gene sequencing.

Long-term fertilization alters soil properties and fungal community composition in fluvo-aquic soil of the North China Plain.

Different fertilization regimes can substantially influence soil fungal community composition, yet fewer studies try to control for the effects of nitrogen input. Here, we investigated the impact of fertilization with equal nitrogen upon soil properties and soil fungal diversity and community composition in the North China Plain in a long-term field experiment. Long-term (32 years) fertilization regimes were applied with equal amounts of nitrogen: no chemical fertilizer or organic manure; chemical fertilization only; organic manure fertilization only, and; combination of 1/2 chemical fertilizer and 1/2 organic manure.

Directed mutation of β-glucanases from probiotics to enhance enzymatic activity, thermal and pH stability.

β-glucanases are widely applied in biological control, brewing and feed industries; however, there are seldom studies of β-glucanases in probiotics. Here, β-glucanase genes were cloned from Bacillus licheniformis, Lactobacillus fermentum and L. johnsonii.

The Mitogen-Activated Protein Kinase Gene Is Involved in Mycoparasitism.

is a mycoparasite used for biocontrol of numerous fungal plant pathogens. Sequencing of the transcriptome of following mycoparasitization of the sclerotia of revealed significant upregulation of a mitogen-activated protein kinase (MAPK)-encoding gene, . Although MAPKs are known to regulate fungal growth and development, the function of in mycoparasitism is unclear.

Bacterial succession and the dynamics of flavor compounds in the Huangjiu fermented from corn.

Huangjiu is one of the Chinese unique and traditional liquor. Corn is a kind of high yield crop in China, which has the characteristics of wide distribution, low price and high starch content. Fermenting Huangjiu with corn not only enrich Huangjiu types, but also opens up a new way for the utilization of corn.

Pontibacter beigongshangensis sp. nov., Isolated from the Mash of Wine.

A Gram-negative, aerobic, oval-shaped, and light red pigmented bacterium, designated T6-1, was isolated from the mash of wine collected from a wine-making laboratory simulated fermenter located in Beijing, China. The optimal growth of T6-1 occurred at 30 °C, pH 7.0 with 1% NaCl.