[F10 expressions in cervical cancer tissues].

Objective: To detect the expression of F10 at both mRNA and protein levels in cervical cancer tissues and explore its role in the occurrence and progression of cervical cancer.

Methods: F10 expressions at mRNA and protein levels were detected in 30 pairs of cervical cancer tissues and adjacent tissues using RT-PCR and immunohistochemistry.

Results: The mRNA and protein expressions of F10 were significantly higher in cervical cancer tissues than in the adjacent normal tissues (P<0.

[Effect of F10 gene silencing and over-expression on cell cycle of choriocarcinoma cell line JAR and the mechanisms].

Objective: To explore the role of F10 gene in regulating cell cycles of choriocarcinoma cells and the underlying mechanisms.

Methods: Using untreated cells as the control, JAR cells with F10 gene silencing or stable F10 over-expression were examined for cell cycle changes by flow cytometry (FCM) and for expressions of cyclin and cyclin-dependent kinase (CDKs) with Western blotting and immunofluorescence technique.

Results: JAR cells over-expressing F10 gene showed reduced duration of cell cycle compared with untreated and with cells after F10 gene silencing.

Dysregulated expression of matrix metalloproteinases and their inhibitors may participate in the pathogenesis of pre-eclampsia and fetal growth restriction.

Background: Trophoblast invasion into the maternal endometrium serves an important function in human pregnancy. Dysregulation of the finely controlled process of trophoblast invasion can result in a wide spectrum of pregnancy abnormalities.

Aims: We aimed to elucidate the relationship between the expression of matrix metalloproteinases and pregnancy complication.

Regulation of trophoblast invasion: the role of matrix metalloproteinases.

Pregnancy success is determined by a complex progress that includes trophoblast invasion and placentation. Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) are metal-dependent endopeptidases capable of degrading extracellular matrix, and appear to play a critical role in trophoblast invasion. This article reviews in detail the role of MMPs, TIMPs, and their regulators in the mechanism of trophoblast invasion in early human pregnancy.

Interleukin-10 may participate in regulating trophoblast invasion in human placentae throughout gestation.

Problem: A successful human pregnancy requires cytotrophoblasts from the fetal portion of the placenta to adopt tumor-like properties. But unlike tumor metastasis, cytotrophoblast invasion is highly regulated both spatially and temporally. The mechanisms that regulate human trophoblast invasion are understood poorly.

[Function of F10 gene, a novel hydatidiform mole-related gene: a preliminary study].

Objective: To study the function of F10 gene, a novel hydaditiform mole-related gene.

Methods: A549 cell line was transfected with the F10 gene of forward or reverse sequence or with the empty vector, respectively. The cellular mRNA was extracted after 24 h of transfection to screen for the differentially expressed genes among the 3 transfected and the control cells using differential display-polymerase chain reaction (ddPCR).

[Construction and identification of a stable eukaryotic expression system for F10 gene].

Objective: To detect the transcriptional level of a novel gene F10 associated with the pathogenesis of hydatidiform mole in human cell lines and screen the cell lines with low F10 expression to construct a stable eukaryotic expression system for F10 gene.

Methods: The expression level of F10 mRNA was detected with fluorescent quantitative PCR in A549, 16HBE, Bel7402, HIC, HepG2, 293, PC and MGC cell lines. A549 cell line was transfected with plasmid pRc-CMV2-F10 via electroporation to allow stable F10 expression, and the positive cell clones were selected by G418.

[Preliminary study of gene expression profiling in human type I and II endometrial carcinoma].

Objective: To study gene expression profiling in human type I and II endometrial carcinoma.

Methods: Six Affymetrix human genome genechips were utilized to investigate the differences in gene expression profiles between type I and II endometrial carcinoma with bioinformatic analysis.

Results: Many genes were highly expressed in estrogen-dependent endometrial carcinoma, and some of them were involved in the metabolism and conversion of estrogen, while some others in estrogen regulation.

[Sequence polymorphism of human papillomavirus type 16 E7 in cervical cancer].

Objective: To explore the genetic polymorphism of E7 open-reading frame of human papillomavirus (HPV) type 16 in cervical cancer.

Methods: The types of HPV was identified by sequence analysis of the PCR product of HPV in cervical cancer tissues. HPV 16 gene fragment in the cervical cancer tissue was amplified by HPV-specific PCR with general consensus primers.

[Significance of pelvic lymph node human papillomavirus DNA detection in cervical cancer].

Objective: To assess the significance of human papillomavirus (HPV) DNA detection in the pelvic lymph nodes in cervical cancer.

Methods: HPV L1 gene fragment was amplified by HPV-specific PCR with general consensus primers from cervical cancer tissues. The types of HPV were identified by sequencing of the PCR product.

[Detection of human papillomavirus DNA in cervical cancer tissue by PCR and direct sequencing].

Objective: To explore an effective method for detecting human papillomavirus (HPV) DNA in cervical cancer tissue.

Methods: HPV L1 gene fragment in cervical cancer tissue was amplified by HPV-specific PCR with consensus primers, and typing of the HPV strains was performed on the basis of sequence analysis of the PCR product.

Results: The positivity rates of HPV DNA was 78% in the 50 cases of cervical cancer, and mixed infection with HPV16 and HPV18 strains was the most common, which accounted for 48% on the total infections.

[Association of the novel hydatidiform mole-related gene F10 with the invasiveness of trophoblastic tumor].

Objective: To study the expressions of the novel gene F10 associated with hydatidiform mole in different trophoblastic tumors and explore the relation of F10 expression with the invasiveness of malignant trophoblastic tumor.

Methods: In situ hybridization was used to study the expression of F10 in 12 cases of hydatidiform mole, 6 cases of invasive mole, and 8 cases of choriocarcinoma.

Results: F10 mRNA was positive in all cases of hydatidiform mole, invasive mole, and choriocarcinoma, and the expression intensity significantly increased in the order of hydatidiform mole, invasive mole and choriocarcinoma (P<0.

[Effect of decidual cell conditioned media on invasion-related gene expression of ovarian tumor cell line COC1].

Objective: To study the effect of decidual cell conditioned media (DCM) on the expression of the invasion-related gene of ovarian tumor cell line COC1.

Methods: After primary culture of the decidual cells of early and late pregnancy, DCM was extracted from the cell cultures for treatment of the ovarian tumor cell line COC1. Analysis of the invasion-related gene expression in COC1 cells was performed by way of reverse transcriptional (RT)-PCR.

[Effects of exogenous tumor necrosis factor alpha on the invasiveness of human trophoblasts].

Objective: To observe the effect of exogenous tumor necrosis factor alpha (TNF-alpha) and its polyclonal antibody (anti-TNF-alpha) on the invasiveness of human trophoblasts in vitro.

Methods: The effect of exogenous TNF-alpha and anti-TNF-alpha on the expression of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinases-2 (TIMP-2) in human trophoblastic cells was investigated with reverse transcription (RT)-PCR (RT-PCR).

Results: The trophoblasts cultured in vitro expressed MMP-2 but not TIMP-2.

DNA microarrays detect the expression of apoptosis-related genes in preeclamptic placentas.

Aims: To investigate the expression of apoptosis-related genes in preeclamptic placentas and the possible mechanism of the regulation process.

Methods: Complementary DNA microarrays were employed to compare gene expression profiles of five preeclamptic and five normal placentas.

Results: Among the 368 genes detected over 35% showed an over 2-fold difference of expression between preeclamptic placentas and normal placentas.

Comparative profiling of metabolism-related gene expression in pre-eclamptic and normal pregnancies.

Extensive endothelial dysfunction has been regarded as the central hallmark in the pathogenesis of pre-eclampsia, but the mechanisms leading to this dysfunction remain unclear. The levels of many metabolism substances, such as lipid peroxides, are changed in plasma of patients with pre-eclampsia. Some of those might be associated with the occurrence of pre-eclampsia.

Expression of transforming growth factor-beta and insulin-like growth factor in molar and placental tissues.

The semiquantitative reverse transcription polymerase chain reaction was employed to detect the expression of transforming growth factor beta (TGF-beta) and insulin-like growth factor (IGF) in complete hydatidiform mole, normal first-trimester villi, the normal term placenta (after vaginal/abdominal deliver) and the preeclamptic placenta at term. The expression of IGF-I mRNA was seen in all five tissues, but its level was much lower in the term placental tissues with preeclampsia than in other tissues. The content of IGF-I mRNA in villous tissues from molar pregnancy was slightly higher than in normal first-trimester villi.

[Role of p38 pathway in PMA-induced in vitro invasion of JAR human choriocarcinoma cell line].

Objective: To investigate the role of p38 mitogen-activated protein kinase (MAPK) signal transduction pathways in regulating the in vitro invasion of JAR human choriocarcinoma cells induced by phorbol 12-myristate 13-acetate (PMA).

Methods: ELISA was used to detect the kinase activity of the JAR cells in response to PMA stimulation, and the in vitro invasion capabilities of the stimulated cells were observed using transwell assay. Changes in the proliferation and activity of the JAR cells cultured in vitro following PMA treatment were also observed by MTT assay.

Comparative study on the expression of cytokine--receptor genes in normal and preeclamptic human placentas using DNA microarrays.

Aims: To study the relationship between the expression levels of cytokine/receptor genes in placenta and the pathogenesis of pre-eclampsia.

Methods: The study was performed to compare the mRNA contents of cytokine (receptor) superfamily genes in placentas from 5 patients with pre-eclampsia and 5 strictly matched normal pregnancies. A complementary DNA microarray representing over 220 cytokine-associated genes was employed to complete the detection.

Effect of polysaccharide Krestin on the up-regulation of macrophage colony-stimulating factor gene expression in protecting mouse peritoneal macrophages from oxidative injury.

Oxidative injury caused by oxidatively modified low density lipoprotein (Ox-LDL) plays an important role in the transformation of macrophages into foam cells and atherogenesis. Treatments to protect macrophages from oxidative injury will be effective in treating atherosclerosis. A macrophage-specific growth factor, macrophage colony-stimulating factor (M-CSF), was reported to be able to prevent the progression of atherosclerosis in Watanabe heritable hypercholesterolemic (WHHL) rabbits.

[Role of phorbol 12-myristate 13-acetate on cytokine expression in JAR trophoblast cell line].

Objective: To investigate whether phorbol 12-myristate 13-acetate (PMA) modifies the invasive ability of trophoblast cells by regulating their cytokine productions.

Methods: Reverse transcriptase-polymerase chain reaction was used to examine the effect of PMA on the expression of cytokines which regulated the invasive ability of trophoblast cells.

Results: Prior to PMA treatment, expressions of the cytokins including hepatocyte growth factor (HGF), interleukin (IL)-1beta, insulin-like growth factor (IGF)-II, transforming growth factor (TGF)- beta and vascular endothelial growth factor (VEGF) were all detected in JAR cells, only with the exception of IGF-I.

Effect of conditioned media from decidual cell culture on the expression of genes regulating the invasion of trophoblastic cells.

Objective: To investigate the effect of the conditioned media from decidual cell cultures (DCM) on the expression of genes regulating the invasion of trophoblastic cells.

Methods: In vitro culture of decidual cells obtained from healthy women of both early (within the first trimester) and full-term pregnancy respectively was performed to prepare conditioned media of decidual cells (DCM). Trophoblastic cells were also obtained in these subjects and treated with DCM for 24 h in in vitro culture, to observe the effect of DCM, with the help of semi-quantitative reverse transcriptase-PCR, on the expression of genes in these cells that regulate their invasion.