Protective effect of thymoquinone on glycation of human myoglobin induced by d-ribose.

Nonenzymatic glycation and the subsequent accumulation of advanced glycation end-products (AGEs) in proteins are factors underlying long-term pathogenesis in diabetes. The study of protein glycation is crucial for elucidating their relationship with diabetes mellitus and related disorders. This study explores the interaction between d-ribose and human myoglobin (HMb), as well as the protective effect of thymoquinone (TQ) on glycation.

Bioactivity-guided isolation of compounds from with antibacterial activity against .

Bioactivity-guided fraction of an extract of to identify antibacterial compounds against , led to the isolation of two new compounds, (2″)-5-methoxy-7-hydroxy-8-lavandulylchromone (13) and (2,)-(-)-sophobiflavonoid CE (19), and 18 known flavonoids, (6a,11a)-(-)-maackiain (1), (2)-(-)-8-prenylnaringenin (2), (2)-(-)-exiguaflavanone K (3), (2)-(-)-sophoraflavanone G (4), (2)-(-)-leachianone A (5), (2)-(-)-kushenol E (6), (2)-(-)-leachianone G (7), (±)-kushenol F (8), (2)-(-)-kurarinone (9), (2)-(-)-kurarinol (10), (2 ,3)- (+)-3,7,4'-trihydroxy-5-methoxy-8-prenylflavanone (11), (2)-(-)-isoxanthohumol (12), (2)-(-)-2'-methoxykurarinone (14), (2 ,3)-(+)-kushenol I (15), calycosin (16), kuraridin (17), (2)-(-)-kushenol A (18), and trifolirhizin (20). Their structures were elucidated based on NMR, MS, and CD spectroscopic analysis. Among them, , , , and exerted modest antibacterial activity against , with MIC of 128-256 μg/mL for and 256-512 μg/mL for , and .

[Mass Transfer Kinetics Model of Ultrasonic Extraction of Pomegranate Peel Polyphenols].

Objective: The dynamic mathematical model of ultrasonic extraction of polyphenols from pomegranate peel was constructed with the Fick's second law as the theoretical basis.

Methods: The spherical model was selected, with mass concentrations of pomegranate peel polyphenols as the index, 50% ethanol as the extraction solvent and ultrasonic extraction as the extraction method. In different test conditions including the liquid ratio, extraction temperature and extraction time, a series of kinetic parameters were solved, such as the extraction process (k), relative raffinate rate, surface diffusion coefficient(D(S)), half life (t½) and the apparent activation energy (E(a)).

[Optimization of SFE-CO2 Extraction for Ursolic Acid from Punica granatum Peel by Plackett-Burman and Central Composite Design].

Objective: The optimum extraction of ursolic acid from Punica granatum peel by SFE-CO2 was investigated.

Methods: Based on the design of Plackett-Burman(PB), significant factors influencing the yield of ursolic acid in the operation process were filtered, with the extraction rate of ursolic acid as the index. The results obtained by steepest ascent method approximated the maximum area of ursolic acid yield.

[The isolation and identification of bone marrow mesenchymal stem cells derived from rabbit mandible].

Purpose: To investigate the methods of isolation, culture and identification of BMMSCs derived from rabbit mandible.

Methods: BMMSCs were collected from rabbit mandible and isolated by density gradient centrifugation. Cells were adherently cultured in vitro, and P2 or P3 BMMSCs populations were collected and examined.

[Study of dahuangzhechong pills on anti-arterial thrombosis with the orthogonal design].

Objective: To screen the main component of Dahuangzhechong pill's anti-arterial thrombosis with the orthogonal design and refine Dahuangzhechong pills.

Methods: In accordance with the orthogonal design table (L(16)2(15)), divided herbs into 16 groups and made the appropriate liquid. The liquid was gave to SD rats by intragastric administration,the model group, normal control group received the same volume of physiological saline.

[An epidemiological study on factors affecting serum thyroglobulin levels].

Objective: To investigate the effects of several factors affecting serum thyroglobulin (TG) levels among people aged 14 or more.

Methods: We selected Panshan with median urinary iodine (MUI) 83.45 micro g/L as a deficient iodine intake community, Zhangwu with MUI 242.