Cloning and characterization of a cDNA encoding 14-3-3 protein with leaf and stem-specific expression from wheat.

The 14-3-3 proteins, originally described as the mammalian brain proteins, are ubiquitous eukaryotic proteins and have been shown to exert an array of function. A great number of 14-3-3 sequences have been reported in Eudicotyledon. The data of 14-3-3 from the monocotyledon plants, however, are limited.

Cloning and characterization of a cDNA encoding Ran binding protein from wheat.

Ran, which functions in nucleocytoplasmic transport and mitosis, binds to and is regulated in part by Ran binding protein (RanBP). A RanBP cDNA (TaRanBP1) was isolated from a wheat cDNA library using RT-PCR product as a probe. The predicted amino acid sequence of TaRanBP1 is over 60% identity to AtRanBP1 from Arabidopsis and also with considerable similarity to human and fungi RanBPs.

Cloning of cDNA encoding COMT from wheat which is differentially expressed in lodging-sensitive and -resistant cultivars.

In the present study two cDNA fragments were cloned by nested-PCR using degenerate primers for COMT and found to be 93% identical at the nucleotide level. The deduced amino acid sequence of the two cDNAs showed a high degree of identity with COMT from other plants and were most similar to COMTs from monocots. RNA gel blot hybridization demonstrated that the wheat COMT gene W-cm5-1 was expressed in stem, root and leaf tissues.

Increased seed cytokinin levels in transgenic tobacco influence embryo and seedling development.

Seed-specific expression of a heterologous vicilin-isopentenyl transferase (ipt) gene has been determined in transgenic tobacco lines 7 and 16. Genetic analysis of self-fertilized progeny showed that a single copy of the vicilin-ipt gene had been integrated, and T2 progeny had become homozygous for the transgene. Stable inheritance of the vicilin-ipt gene in T3 progeny was verified by polymerase chain reaction analysis.