Parallel evolution of methyltransferases leads to vobasine biosynthesis in and .

Monoterpenoid indole alkaloids (MIA) are one of the largest and most complex alkaloid class in nature, boasting many clinically significant drugs such as anticancer vinblastine and antiarrhythmic ajmaline. Many MIAs undergo nitrogen -methylation, altering their reactivity and affinity to the biological targets through a straightforward reaction. Remarkably, all known MIA -methyltransferases (NMT) originate from the neofunctionalization of ancestral γ-tocopherol -methyltransferases (γTMTs), a phenomenon seemingly unique to the Apocynaceae family.

Antibiotics in the rice-crayfish rotation pattern: Occurrence, prioritization, and resistance risk.

Antibiotics are extensively utilized in aquaculture to mitigate diseases and augment the productivity of aquatic commodities. However, to date, there have been no reports on the presence and associated risks of antibiotics in the emergent rice-crayfish rotation (RCR) system. This study investigated the occurrence, temporal dynamics, prioritization, sources, and potential for resistance development of 15 antibiotics within the RCR ecosystem.

Fate of high-risk antibiotic resistance genes in large-scale aquaculture sediments: Geographical differentiation and corresponding drivers.

Antibiotic resistance genes (ARGs), emerging environmental contaminants, have become challenges of public health security. However, the distribution and drivers of ARGs, especially high-risk ARGs, in large-scale aquaculture sediments remain unknown. Here, we collected sediment samples from 40 crayfish ponds in seven main crayfish culture provinces in China and then investigated the distribution and risk of ARGs based on high-throughput sequencing and quantitative PCR techniques.

Occurrence, distribution, and ecological risks of antibiotics in Honghu Lake and surrounding aquaculture ponds, China.

Antibiotics are largely applied in aquaculture to increase production and control diseases, while how the antibiotics used in pond farming influence the distribution of antibiotics in receiving water seasonally is still not well understood. In this study, the variations of 15 frequently used antibiotics in Honghu Lake and surrounding ponds were investigated seasonally to figure out the impact of pond farming on antibiotics distributions in Honghu Lake. Results showed that the antibiotic concentrations in fish ponds ranged from 11.

Improved protein glycosylation enabled heterologous biosynthesis of monoterpenoid indole alkaloids and their unnatural derivatives in yeast.

With over 3000 reported structures, monoterpenoid indole alkaloids (MIAs) constitute one of the largest alkaloid groups in nature, including the clinically important anticancer drug vinblastine and its semi-synthetic derivatives from (Madagascar's periwinkle). With the elucidation of the complete 28-step biosynthesis for anhydrovinblastine, it is possible to investigate the heterologous production of vinblastine and other medicinal MIAs. In this study, we successfully expressed the flavoenzyme -acetylstemmadenine oxidase in (baker's yeast) by signal peptide modification, which is a vinblastine biosynthetic gene that has not been functionally expressed in this system.

Distribution and influencing factors of antibiotic resistance genes of crayfish (Procambarus clarkii) intestine in main crayfish breeding provinces in China.

The propagation of antibiotic resistance genes (ARGs) has become a global public health concern. However, the distribution and influencing factors of ARGs, especially high-risk ARGs, in the gut of aquaculture animals remain unclear. Here, we employed 16S rRNA gene sequencing and high-throughput quantitative PCR techniques to determine crayfish gut microbiota and ARGs collected from 40 culture ponds in major crayfish farming provinces of China.

Characteristics of (micro)plastic transport in the upper reaches of the Yangtze River.

Many studies focus on the transport of plastic from rivers to oceans while little attention was paid to the plastic transport in the upper reaches of rivers. Transport process of plastic from upstream to downstream in the whole river basin scale is still poorly understood. In this work, five sections in the upper reaches of the Yangtze River were investigated to characterize the features of plastic transport.

Manipulation of fish community structure effectively restores submerged aquatic vegetation in a shallow subtropical lake.

Fish community manipulation and regulation has been largely overlooked as a mitigation strategy for restoring submerged aquatic vegetation (SAV) in shallow lakes of the middle and lower Yangtze River Basin (MLYRB). An in-situ fish exclusion experiment and a large-scale lake manipulation were conducted to test the hypothesis that the reasonable removal of benthivorous and herbivorous fish would facilitate the restoration and reconstruction of SAV in shallow lakes within the MLYRB. The in-situ exclusion experiment was conducted from April to October in 2017.