Identification of Polar Constituents in the Decoction of Juglans mandshurica and in the Medicated Egg Prepared with the Decoction by HPLC-Q-TOF MS².

As a folk medicinal plant, has been used for the treatment of cancer in China and Korea. Traditionally, is decocted together with chicken eggs. Both the decoction and medicated eggs possess anti-tumor properties.

Multiconstituent identification in root, branch, and leaf extracts of Juglans mandshurica using ultra high performance liquid chromatography with quadrupole time-of-flight mass spectrometry.

As a traditional medicinal plant, Juglans mandshurica has been used for the treatment of cancer. Different organs of this plant showed anti-tumor activity in clinic and laboratory. Comparative identification of constituents in different plant organs is essential for investigation of the relationship between chemical constituents and pharmacological activities.

[Studies on optimizing extraction technics of flowers of Polygonum orientale by response surface methodology].

Objective: To analyze and optimize extraction technics of Polygonum orientale flowers by response surface methodology.

Methods: With the index for the content of taxifolin in flowers of Polygonum orientale, the effect of three factors such as concentration of alcohol, extraction time and solvent-solid ratio was designed by Box-Behnken central composite. Extraction technic parameters of Polygonum orientale flowers was optimized by response surface methodology.

Role of berberine in anti-bacterial as a high-affinity LPS antagonist binding to TLR4/MD-2 receptor.

Background: Berberine is an isoquinoline alkaloid mainly extracted from Rhizoma Coptidis and has been shown to possess a potent inhibitory activity against bacterial. However, the role of berberine in anti-bacterial action has not been extensively studied.

Methods: The animal model was established to investigate the effects of berberine on bacterial and LPS infection.

[Determination of taxifolin in Polygonum orientale of different storage period].

Objective: To find out the correlation between the content of taxifolin in Polygonum orientale and the storage time.

Method: HPLC was used to determine taxifolin. The chromatographic condition was as following: Diamonsil C18 column (4.

[Study on species identification of commercial drug cudraniae tricuspidatae radix et caulis and its medicinal parts].

Objective: To establish an identification method of Cudrania tricuspidata and Cudrania cochinchinensis, and their medicinal parts, and analyse the species of commercial drug Cudraniae Tricuspidatae Radix et Caulis.

Methods: TLC and First-order Derivative UV Spectrophotometry were used.

Results: Commercial drugs 5, 6, 7 and 9 belonged to the stem of Cudrania cochinchinensis, commercial drug 1, 2, 3 ,4 and 8 were neither Cudrania tricuspidata nor Cudrania cochinchinensis.

[Simultaneous determination of four flavones in root and stem of Cudrania tricuspidata and C. cochinchinensis by HPLC-DAD].

Objective: To establish a HPLC-DAD method for the determination of axifolin, naringenin, quercetin and kaempferol in Cudrania tricuspidata and C. cochinchinensis in order to provide a scientific reference for species identification and quality evaluation, by establishing.

Method: The determination was performed by HPLC-DAD on an Agilent C18 column (4.

[Effects of different processing on active components in fructus polygoni orientalis by HPLC analysis].

Objective: To study the effects of different processing on taxifolin and quercetin in Fructus Polygoni Orientalis.

Methods: The analysis was performed on a Agilent column (4. 6 mm x 150 mm, 5 microm) eluted with a gradient elution of methanol-water containing 0.

[The analysis for silver iodide fine particles of TLC/FTIR matrix].

In situ TLC/FTIR technique has tremendous potential in the analysis of complex mixtures. However, the progress in this technique was quite slow. The reason is that conventional stationary phase has strong absorption in FTIR spectrum and thus brings about severe interference in the detection of samples.

[Preliminary investigation on the formation mechanism of CCL4-water-cetyl trimethyl ammonium bromide (CTAB) gel].

Gels are gaining extensive interest owing to their versatile applications in fields such as drug delivery, tissue engineering, cosmetics, templated materials and food industry. Surfactants have an ability to self-assemble into a variety of supramolecular aggregate structures and morphologies. Of particular interest in resent years are surfactant-based gels, one special class of materials due to surfactant assemblies resulting in viscoelastic solid-like rheological behaviors.

[Study on the chemical constituents of Cudrania tricuspidata].

Objective: To study on the chemical constituents of Cudrania tricuspidata.

Methods: The chemical constituents were separated by means of silica gel and Sephadex LH-20 chromatography, and their structures were identified by spectra of 1H-NMR, 13 C-NMR.

Results: Seven compounds were isolated and identified as Quercetin (I),5,7,4'-trihydroxyldihydroisoflavone (II), (13alpha, 14beta, 17alpha, 20R)-Lanosta-7, 24-diene-3beta-ol (III), (13alpha, 14beta,17alpha, 20R)-Lanosta-7,24-diene-3beta-0-acetate (IV), Taxifolin (V), Dihydromorin (VI), Cycloartocarpin(VII).

[Study on the expressed oil of princes-feather fruit before and after processed by GC-MS].

Objective: To compare the chemical compositions in the expressed oil before and after processed of princes-feather fruit.

Methods: Making use of the GC-MS to compare the chemical composition in the expressed oil before and after processed of princes-feather fruit.

Results: 4 chemical compounds were detected in the expressed oil of the raw and 7 of the processed.

HPLC determination of malondialdehyde in ECV304 cell culture medium for measuring the antioxidant effect of vitexin-4''-O-glucoside.

To investigate the antioxidant effect of vitexin-4''-O-glucoside, a flavone glycoside, isolated from the leaves of Crataegus pinnatifida Bge. var. major, we developed a simple and sensitive high-performance liquid chromatography (HPLC) method to determine levels of malondialdehyde (MDA) in ECV304 cell culture medium after induction by tert-butyl-hydroperoxide (TBHP).