Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification.

The large-scale preparation of Polyehylene terephthalate (PET) hydrolysing enzymes in low-cost is critical for the biodegradation of PET in industry. In the present study, we demonstrate that the post-translational glycosylation of Pichia pastoris makes it a remarkable host for the heterologous expression of PETase from Ideonella sakaiensis 201-F6 (IsPETase). Taking advantage of the abundant N- and O-linked glycosylation sites in IsPETase and the efficient post-translational modification in endoplasmic reticulum, IsPETase is heavily glycosylated during secretory expression with P.

Intestinal Flora Metabolite Trimethylamine Oxide Is Inextricably Linked to Coronary Heart Disease.

Atherosclerotic coronary heart disease is a common cardiovascular disease with high morbidity and mortality. In recent years, the incidence of coronary heart disease has gradually become younger, and biomarkers for predicting coronary heart disease have demonstrated valuable clinical prospects. Several studies have established an association between coronary heart disease and intestinal flora metabolites, including trimethylamine oxide (TMAO), which has attracted widespread attention from researchers.

Clinical Feasibility and Safety of Scleral Collagen Cross-Linking by Riboflavin and Ultraviolet A in Pathological Myopia Blindness: A Pilot Study.

Introduction: To investigate the feasibility and safety of scleral ultraviolet A (UVA) cross-linking (scleral CXL) on pathologically blindness.

Methods: This was a prospective, observational clinical study. Five patients with monocular blindness due to pathological myopic maculopathy were enrolled.

A systematic review on cross-culture, humor and empathy dimensions in conversational chatbots: the case of second language acquisition.

The advancement of information and communication technologies has led to an increasing use of conversational chatbots in the learning and teaching sector, especially for the second language (L2) acquisition. In the field of second language acquisition, the use of AI chatbots has been explored, mainly studying pedagogical approaches. However, there is a limited study in the development of empathetic strategies for dealing with learners' emotional discomfort, the impact of humor and the consideration of learners' cultural backgrounds.

Investigation of the Temperature Dependence of the Chemical-Mechanical Properties of the Wufeng-Longmaxi Shale.

Shale rocks have been widely investigated to evaluate the productivity of oil/gas. The high temperature generated by the explosive fracturing to stimulate the gas reservoir has a significant impact on the chemical-mechanical properties of shale rocks. Pioneering works have been carried out at temperatures below 500 °C, but little has been done to quantify the correlation between the chemical and mechanical properties of shale at temperatures above 500 °C.

Bifurcation Control on the Un-Linearizable Dynamic System via Washout Filters.

Information fusion integrates aspects of data and knowledge mostly on the basis that system information is accumulative/distributive, but a subtle case emerges for a system with bifurcations, which is always un-linearizable and exacerbates information acquisition and presents a control problem. In this paper, the problem of an un-linearizable system related to system observation and control is addressed, and Andronov-Hopf bifurcation is taken as a typical example of an un-linearizable system and detailed. Firstly, the properties of a linear/linearized system is upon commented.

Chest pain with ST-elevation in leads AVR, III, AVF, V, V, V and V.

We mistakenly diagnosed a patient with pulmonary embolism as ST-elevation acute myocardial infarction. The case complained of chest pain with ST-elevation in leads AVR, III, AVF, V, V, V and V. In addition, we also found ST- elevation >ST- elevation , Q>Q and Q.

Sprayer boom height measurement in wheat field using ultrasonic sensor: An exploratory study.

In order to explore the influencing factors and laws of ultrasonic sensor detecting wheat canopy height, designed an ultrasonic sensor detection height test platform with speed adjustable function. Taking step surface, bare soil and wheat canopy as the research objects, a canopy height calculation method based on K-mean clustering is proposed, and the response characteristics of ultrasonic detection to three media under different operating speeds are explored. Firstly, the step detection test results show that the average detection error of ultrasonic sensor is 1.

Ciprofol attenuates the isoproterenol-induced oxidative damage, inflammatory response and cardiomyocyte apoptosis.

Ciprofol (HSK3486), a novel 2,6-disubstituted phenol derivative, is a new intravenous anesthetic compound with a similar chemical structure to propofol. Animal studies have also shown that propofol plays a protective role in a variety of cardiovascular diseases, including myocardial infarction, myocardial ischemia-reperfusion injury and takotsubo syndrome. However, whether ciprofol exerts cardioprotective effects on myocardial infarction remains unclear.

Global, regional, and national deaths, disability-adjusted life years, years lived with disability, and years of life lost for the global disease burden attributable to second-hand smoke, 1990-2019: A systematic analysis for the Global Burden of Disease Study.

Background: Smoke-free policies have led to a decline in smoking prevalence. Nevertheless, as the global population grows, more non-smokers are exposed to second-hand smoke (SHS) hazards. Mitigating SHS hazards requires a systematic analysis of the global disease burden attributable to SHS.

Ant nest-like hierarchical porous imprinted resin-dispersive solid-phase extraction for selective extraction and determination of polychlorinated biphenyls in milk.

Polychlorinated biphenyls (PCBs) are persistent toxic, organic chemicals that tend to accumulate in the food chain. This study reports the rapid and selective extraction and determination of PCBs (PCB81, 153, 105, 126, and 157) in milk samples by a dispersive solid-phase extraction (DSPE) coupled with gas chromatography-tandem mass spectrometry (GC-MS/MS). An ionic liquid-molecularly imprinted porous resin (IL-MIPPR) as a DSPE adsorbent was synthesized from m-aminophenol, formaldehyde, and 2,2'-benzidinedisulfonic acid as the monomer, crosslinker, and virtual template, respectively.

Experimental investigation of dynamic mechanical characteristics of inhomogeneous composite coal-sandstone combination for coalbed methane development.

In deep resource exploitation, the coal seam and the strata are jointly loaded, forming a systematic combined structure that can have a significant effect on coalbed methane (CBM) development. Therefore, to understand the deformation and damage characteristics due to blasting load of the complex and real ground conditions, the 50 mm split Hopkinson pressure bar (SHPB) was used to study the dynamic performance and energy changes of coal-sandstone combination and sandstone-coal combination as inhomogeneous materials. In addition, high-speed photographic equipment was employed to determine the damage failure mechanisms.

The effect of the street environment on two types of essential physical activity in industrial neighborhoods from the perspective of public health: a study from the Harbin low-income population health survey, China.

A large number of low-income residents in industrial neighborhoods rarely engage in recreational and physical activities in green spaces in extremely cold weather. This study mainly explores the relationship between the street environment and physical activities under special industrial properties and extreme cold weather conditions. In addition, we further divide essential physical activity into two categories, life-type and traffic-type physical activity, to explore and refine the related studies.

A Stable Rechargeable Aqueous Zn-Air Battery Enabled by Heterogeneous MoS Cathode Catalysts.

Aqueous rechargeable zinc (Zn)−air batteries have recently attracted extensive research interest due to their low cost, environmental benignity, safety, and high energy density. However, the sluggish kinetics of oxygen (O2) evolution reaction (OER) and the oxygen reduction reaction (ORR) of cathode catalysts in the batteries result in the high over-potential that impedes the practical application of Zn−air batteries. Here, we report a stable rechargeable aqueous Zn−air battery by use of a heterogeneous two-dimensional molybdenum sulfide (2D MoS2) cathode catalyst that consists of a heterogeneous interface and defects-embedded active edge sites.

Silica nanoparticle remodeling under mild conditions: versatile one step conversion of mesoporous to hollow nanoparticles with simultaneous payload loading.

A binary mixture of mesoporous silica nanoparticles plus organic polyammonium additive (dye or drug) is cleanly converted upon mild heating into hollow nanoparticles. The remodeled nanoparticle shell is an organized nanoscale assembly of globular additive/silica subunits and cancer cell assays show that a loaded drug additive is bioavailable.

Intestinal Flora Derived Metabolites Affect the Occurrence and Development of Cardiovascular Disease.

In recent years, increasing evidence has shown that the gut microbiota and their metabolites play a pivotal role in human health and diseases, especially the cardiovascular diseases (CVDs). Intestinal flora imbalance (changes in the composition and function of intestinal flora) accelerates the progression of CVDs. The intestinal flora breaks down the food ingested by the host into a series of metabolically active products, including trimethylamine N-Oxide (TMAO), short-chain fatty acids (SCFAs), primary and secondary bile acids, tryptophan and indole derivatives, phenylacetylglutamine (PAGln) and branched chain amino acids (BCAA).

Pressure-Dependent Structural and Band Gap Tuning of Semiconductor Copper(I) Thiocyanate (CuSCN).

Copper(I) thiocyanate (CuSCN) is a p-type semiconductor with exceptional properties for optoelectronic devices such as solar cells, thin-film transistors , organic light-emitting diodes, etc. Understanding the structure-optical property relationships in CuSCN is critical for its optoelectronic applications. Herein, high-pressure techniques combined with theoretical calculations are used to thoroughly investigate the structural and optical changes of CuSCN upon compression.

Seismic response of benchmark high-speed rail (HSR) round-ended rectangular-shaped cross-section solid (RERSCSS) concrete pier based on the shaking table tests.

High-speed rail (HSR) has recently expanded its networks globally, but its 350 km/h bridges have not yet been tested for high-level earthquakes. This study tests the typical HSR bridge on a shaking table to assess the seismic performance in high-level earthquakes such as Maximum Considered Earthquake. Based on the model similarity theory, it creates nine round-ended rectangular-shaped cross-section solid RC HSR bridge piers.

Quantitative analysis of Chinese steamed bread staling using NIR, MIR, and Raman spectral data fusion.

For efficient and comprehensive detection of the staling degree of Chinese steamed bread (CSB), staled CSB samples stored for 0-16 days were prepared and analyzed using near-infrared (NIR), mid-infrared (MIR), and Raman spectroscopy combined with data fusion. Among three data fusion schemes, decision-level fusion achieved the best performance when quantifying the CSB staling degree according to the soluble starch amylose fraction, relative crystallinity, and hardness, with determination coefficients and root mean square errors for the validation set in the range of 0.928-0.

Association Between Aromatase Inhibitors and Myocardial Infarction Morbidity in Women With Breast Cancer: A Meta-Analysis of Observational Studies.

Background: The cardiovascular toxicity of aromatase inhibitors (AIs) for women with estrogen receptor-positive breast cancer is controversial. We aimed to evaluate the association between AIs and the risk of myocardial infarction (MI) in women with estrogen receptor-positive breast cancer based on real-world studies.

Method: PubMed, Embase, and Cochrane Library were searched to identify studies that estimated the association between MI risk and AIs.

An isolable germylyne radical with a one-coordinate germanium atom.

Carbynes (R-[Formula: see text]), species that bear a monovalent carbon atom with three non-bonding valence electrons, are important intermediates and potentially useful in organic synthetic chemistry. However, free species of the type R-[Formula: see text] of any group 14 element (E) have eluded isolation in the condensed phase due to their high reactivity. Here we report the isolation, characterization and reactivity of a crystalline germylyne radical by using a sterically hindered hydrindacene ligand.

Temperature effect on cable force of a special-shaped tied-arch bridge.

In this study, on-site monitoring was used to obtain the temperature field distribution and cable force values of the bridge at various times, the temperature change pattern of the bridge elements, the temperature sensitivity of the cable force of different parts of the bridge, and the trend of the cable force under temperature load were all analyzed. The static response of the wires was studied under various operating circumstances. The total cable force of the bridge is inversely related to the temperature rise and fall, and the temperature sensitivity of the cable force in different parts of the bridge is different under the same temperature effect.

[Effect of herbal cake-separated moxibustion on behavioral stress reactions and blood lactic acid level and muscular AMPK/PGC-1α signaling in rats with chronic fatigue syndrome].

Objective: To observe the effect of herbal cake-separated moxibustion (HCSM) on serum lactic acid (BLA) level and AMPK/PGC-1α signaling pathway in the quadriceps femoris in chronic fatigue syndrome (CFS) rats, so as to explore its mechanisms underlying improvement of CFS.

Methods: According to the random number table, 50 SD rats were divided into blank control, model, HCSM, sham HCSM and medication (herbal medicine gavage) groups, with 10 rats in each group. The CFS model was established by using chronic restraint and exhaustive swimming, alternately, once daily for 21 days.

Addressing the imaging limitations of a microsphere-assisted nanoscope.

In the past decade, microsphere-assisted nanoscopy has been developed rapidly to overcome the diffraction limit. However, due to the limited size and high surface curvature of microspheres, the magnified imaging still suffers from problems like limited view scope, imaging distortion, and low contrast. In this paper, we specialize in the imaging mechanism of microspheres and find irradiance as the key factor for microsphere imaging quality.