[Enzyme immunoassay of ampicillin in milk].

An indirect immunoassay for quantitative determination of ampicillin (range, 10-1000 ng/ml) in buffer or milk has been developed. Polyclonal antibodies were obtained against ampicillin conjugated with bovine serum albumin; the conjugate was synthesized by direct condensation using carbodiimide. The antibodies were specific for ampicillin and exhibited low cross-reactivity to other penicillins (azlocillin, 17%; penicillin G, 10%; piperacillin, 5%; and carbenicillin, 4%).

[Optimization of a method of solid-phase immunoenzyme analysis for determination of chloramphenicol in milk].

The method of solid-phase enzyme linked immunosorbent assay (ELISA) for quantitative detection of chloramphenicol (CAP) in milk was developed. Peculiarities of the adsorption on the microtitre plates surface of CAP-ovalbumin conjugate were investigated. Different conditions of competition stage of the analysis were studied.

[Solid-phase immunoenzyme analysis of chloramphenicol in human blood serum].

The method of enzyme linked immunosorbent assay (ELISA) for quantitative detection of chloramphenicol (CAP) in human blood serum was developed. Peculiarities of the adsorption on the microtitre plates surface of CAP-ovalbumin conjugate were investigated. Different conditions of competition stage of the analysis were studied.

[Development of a solid-phase immunoenzyme analysis of gentamicin in human blood serum].

An enzyme immune test system was designed and optimized for quantitative assay of gentamicin in human sera. Immunospecific reagents i.e.

[Polarization fluoroimmunoassay of propazine in reversed micelles of aerosol OT in octane].

The interaction between fluorescein-labeled propazine and antibodies against this hapten was studied in the reversed micelles of Aerosol OT in n-octane by a polarization fluoroassay. The effect of the hydration degree of micelles W0 (W0=[H2O]/[Surf]), which determines their size and surfactant concentration, on the binding of the antigen with antibodies was studied. A high hydration degree of the reversed micelles (W0 = 15-30) and low concentration of the surfactant (less than 50 microM) are optimal for binding.

[The problem of selecting the structure of a labelled antigen in developing a fluorescence polarization immunoassay for atrazine].

Influence of labelled antigens structure on the sensitivity of the polarization fluoroimmunoassay of atrazine was studied. It is shown that the highest sensitivity is provided by the use of the heterologous labelled reagent with the shortest chemical bridge between the antigen and fluorescent label.

[Polarization fluorescence immunoanalysis of herbicides of the S-1,3,5-triazine class].

Polarization fluoroimmunoassay for s-triazine herbicides was developed. The sensitive of assay is higher with using shortest chemical "bridge" length between the molecule of antigen and fluorescent label. The detection limit of atrazine in 50 microL sample was 6 ng/ml.