[Coordination of the changes in some sites of the hantaviral segments].

Correlation in the changes of the nucleotide and amino acid sequences at the Hantaan virus occurring in different parts of S- and M-segments were studied. The correlation was analyzed using comparison of substitutions in aligned nucleic sequences of the segments. Correlations of the nucleotide substitutions between S- and M-segments were observed.

[Related substitutions in the small segment of Old World hantavirus genome].

Whether there were related nucleotide substitutions in the S-segments of the genome of Tula, Puumala, Hantaan, Dorava/Belgrad, and Seoul viruses was studied. The significance of the correlations found in nucleotide substitutions was estimated by the level of mutual information. The majority of the correlated changes were synonymous substitutions.

[Study of the phylogenetic relations of Puumala hantaviruses, by using the mutual information calculating method].

Phylogenetic relations were investigated in the group of Puumala viruses (the genus Hantavirus, the family Bunyaviridae) by the mutual information showing the similarity of the nucleotide sequences of S- and M-genome segments. There was evidence that recombination events might occurr in some Eurasian Puumala viruses (Scandinavian and West-Siberian genotypes). The investigation revealed the conservative sites available in the S and M segments that were common to most Puumala viruses, as well as the preservation of alternation and mutual arrangement of these sites in the S and M segments even for greatly diverging genotypes, which may be used in the detection of recombination events in Puumala viruses during further studies.

[Study of phylogenetic relations of hantaviruses by using the mutual information method. Tula genotype viruses].

Phylogenetic relations were studied in the group of Tula viruses (Hantavirus genus, Bunyaviridae family) of Eurasia. The similarity in the nucleotide sequences of segments was estimated by the value of mutual information. The application of this method confirmed that recombinant events might occur in some Tula-like viruses of Eurasia, as previously presumed by the results of bootscanning and analysis of amino acid sequences.