Protective immune response of oral rabies vaccine in stray dogs, corsacs and steppe wolves after a single immunization.

In this study the safety and protective immunity of an oral rabies vaccine, based on the live, modified rabies virus strain VRC-RZ2, was examined in stray dogs (Canis Sp.), corsacs (Vulpes corsac) and steppe wolves (Canis lupus campestris). In the safety group (dogs, n=6; corsacs, n=3; wolves, n=3) which was vaccinated with a 10-times field dose/animal, no animals showed any signs of disease or changes in behavior or appetite during the period of clinical observation, similar to the animals in the negative control group.

[Universal diagnostic oligonucleotide microarray for subtyping of human and animal influenza A viruses].

The diagnostic oligonucleotide microarray for subtyping of human and animal influenza A viruses (IAVs) was developed. We proposed a simple method of the fluorescent labeling of genomic segments of all known IAVs subtypes, the composition of the hybridization buffer, as well as the software of the data processing. 48 IAVs strains of different subtypes were analyzed using our microarray.

[The development and application of solid-phase ELISA for the identification of avian influenza virus and its subtypes H5 and N3 in different biological samples].

The paper presents the results of research on the development of effective solid-phase ELISA methods for the identification of avian influenza virus (AIV) and typing its H5 and N3 subtypes in different biological samples. Optimized schemes for the production of goat antisera to matrix protein, hemagglutinin H5 and neuraminidase N3 of AIV were proposed. They were used to produce active and specific immunoglobulins and conjugates.