Spatiotemporally resolved mapping of extracellular proteomes via in vivo-compatible TyroID.

Extracellular proteins play pivotal roles in both intracellular signaling and intercellular communications in health and disease. While recent advancements in proximity labeling (PL) methods, such as peroxidase- and photocatalyst-based approaches, have facilitated the resolution of extracellular proteomes, their in vivo compatibility remains limited. Here, we report TyroID, an in vivo-compatible PL method for the unbiased mapping of extracellular proteins with high spatiotemporal resolution.

Glycosylated Phosphorous Corrole as effective Photosensitizers for Cancer Photodynamic Therapy via Enhancing cellular Uptake.

Developing effective photosensitizers (PSs) is the most important for cancer photodynamic therapy (PDT) treatment. Phosphrous corrole complex is a kind of promising photosensitizer. Herein, we report the synthesis and anticancer activity of a novel glycosylated phosphorous(V) corrole PS 5,15-bis(pentafuorophenyl)-10-(4-glycosyl phenyl) corrole phosphorous complex (2-P) which was prepared from the glycosylation of phosphorous 5,15-bis(pentafuorophenyl)-10-(4-oxhydryl phenyl) corrole (1-P).

Efficacy and safety of sintilimab combined with trastuzumab and chemotherapy in HER2-positive advanced gastric or gastroesophageal junction cancer.

Background: To evaluate the efficacy and safety of sintilimab in combination with trastuzumab and chemotherapy for HER2-positive advanced gastric/gastroesophageal junction cancer (GC/GEJC).

Methods: HER2-positive advanced GC/GEJC patients admitted to our department between January 2018 and October 2024 were included in this study. Patients who received sintilimab in combination with trastuzumab and chemotherapy were assigned to cohort A, while patients who received trastuzumab and chemotherapy alone were assigned to cohort B.

Role of nucleotide pair frequency and synonymous codon usage in the evolution of bovine viral diarrhea virus.

Synonymous codon usage plays an important role in the adaptation of viruses to their hosts. Bovine viral diarrhea virus (BVDV) relies on a high mutation rate in its genome to achieve the necessary fitness in a particular host. However, the question of which selective forces influence nucleotide pair and synonymous codon usage patterns in different BVDV genotypes remains unresolved.

Real-World Emissions and Formation Mechanism of IVOCs from Biodiesel-Fueled Agricultural Machinery.

The use of biodiesel as an alternative to diesel remains controversial because of its potential impact on organic matter emissions. Herein, intermediate-volatility organic compounds (IVOCs) from five agricultural machines (AMs) using three types of fuels (pure diesel (B0), diesel mixed with 5 and 20% biodiesel (B5 and B20), respectively) were collected and analyzed. The following results were obtained: (1) Reasonable biodiesel ratios reduced IVOC emissions.

Efficacy and safety of loxoprofen sodium cataplasms in the treatment of osteoarthritis: A randomized, multicenter study.

The present study aimed to compare the efficacy and safety of loxoprofen sodium cataplasm (LSC) with those of flurbiprofen cataplasm (FPC) in osteoarthritis (OA) treatment. In this multicenter, randomized controlled trial, subjects meeting the inclusion and exclusion criteria were randomly assigned to the two treatment groups. According to the manufacturer's instructions, the first group received LSC once daily, with the application of one patch per area for 2 weeks, whereas the second group received FPC twice daily, with the application of one patch per area for 2 weeks.

Fructose induces inflammatory activation in macrophages and microglia through the nutrient-sensing ghrelin receptor.

High fructose corn syrup (HFCS) is a commonly used sweetener in soft drinks and processed foods, and HFCS exacerbates inflammation when consumed in excess. Fructose, a primary component of HFCS; however, it is unclear whether fructose directly activates inflammatory signaling. Growth hormone secretagogue receptor (GHSR) is a receptor of the nutrient-sensing hormone ghrelin.

Discovery of Covalent and Cell-Active ALKBH5 Inhibitors with Potent Antileukemia Effects In Vivo.

The N-methyladenosine (mA) demethylase ALKBH5 is the only other identified mA eraser except for FTO, and dysregulated ALKBH5 functions were closely associated with leukemogenesis. However, the development of ALKBH5 inhibitors is slow compared to FTO inhibitors. Inspired by a non-catalytic C200-covalent strategy, a series of maleimide derivatives were designed and synthesized as potent and covalent ALKBH5 inhibitors in this work.

Histone Deacetylase Inhibitor Largazole Deactivates A Subset of Superenchancers and Causes Mitotic Chromosome Mis-alignment by Suppressing SP1 and BRD4.

Histone deacetylase inhibitors have been investigated as potential therapeutic agents for cancer and other diseases. HDIs are known to promote histone acetylation, resulting in an open chromatin conformation and generally increased gene expression. In previous work, we reported that a subset of genes, particularly those regulated by superenhancers, can be suppressed by the HDAC inhibitor largazole.

Disrupted Mitochondrial Dynamics Impair Corneal Epithelial Healing in Neurotrophic Keratopathy.

Neurotrophic keratopathy (NK) is a degenerative corneal disease characterized by impaired corneal sensitivity and epithelial repair that is often linked to sensory nerve dysfunction. To establish a clinically relevant model and explore the mechanisms underlying NK pathogenesis, we developed a novel mouse model through partial transection of the ciliary nerve. This approach mimics the progressive nature of NK, reproducing key clinical features such as corneal epithelial defects, reduced sensitivity, diminished tear secretion, and delayed wound healing.

Global, regional, and national burden inequality of chronic kidney disease, 1990-2021: a systematic analysis for the global burden of disease study 2021.

Background: Chronic kidney disease (CKD) is a significant global health issue, often linked to diabetes, hypertension, and glomerulonephritis. However, aggregated statistics can obscure heterogeneity across subtypes, age, gender, and regions. This study aimed to analyze global CKD trends from 1990 to 2021, focusing on age, gender, socio-demographic index (SDI), and regional variations.

Application of Deep Learning Accelerated Image Reconstruction in T2-weighted Turbo Spin Echo Imaging of the Brain at 7T.

Prolonged imaging times and motion sensitivity at 7T necessitate advancements in image acceleration techniques. This study evaluates a 7T deep-learning (DL)-based image reconstruction using a deep neural network trained on 7T data, applied to T2-weighted turbo spin echo imaging. Raw k-space data from 30 consecutive clinical 7T brain MRI patients was reconstructed using both DL and standard methods.

Interaction of micro and nanoplastics (MNPs) with agricultural stored products and their pests.

Micro and nanoplastics (MNPs) pose significant environmental concerns due to their potential implications for ecosystems and human health. While previous research has primarily focused on the environmental impacts (aquatic ecosystem, soil health) of MNPs, this review investigates their interactions with agricultural stored products, specifically their effects on stored product pests and grain quality. MNPs can infiltrate grains through various pathways, including atmospheric deposition, plastic residues from cultivation, and pest activity.

Completely conserved VP2 residue K140 of KREMEN1-dependent enteroviruses is critical for virus-receptor interactions and viral infection.

Unlabelled: The KREMEN1 (KRM1) protein is a cellular receptor for multiple enteroviruses that cause hand, foot, and mouth disease (HFMD), including coxsackievirus CVA2, CVA3, CVA4, CVA5, CVA6, CVA10, and CVA12. The molecular basis for the broad recognition of these viruses by the KRM1 receptor remains unclear. Here, we report the indispensable role of the completely conserved VP2 capsid protein residue K140 (designated K2140) in mediating receptor recognition and infection by CVA10 and other KRM1-dependent enteroviruses.

Study on the Carrier Transport Process in Deep Ultraviolet Light-Emitting Diodes with Al-Content-Varied AlGaN Composite Last Quantum Barrier.

Serious electron leakage and poor hole injection efficiency are still challenges for deep ultraviolet AlGaN-based light-emitting diodes with a traditional structure in achieving high performance. Currently, the majority of research works concentrate on optimizing the structures of the electron blocking layer (EBL) and last quantum barrier (LQB) separately, rather than considering them as an integrated structure. Therefore, in this study, an Al-content-varied AlGaN composite last quantum barrier (CLQB) layer is proposed to replace the traditional EBL and LQB layers.

Epithelial stem cells from human small bronchi offer a potential for therapy of idiopathic pulmonary fibrosis.

Background: Idiopathic pulmonary fibrosis (IPF) is a fibrosing interstitial pneumonia with restrictive ventilation. Recently, the structural and functional defects of small airways have received attention in the early pathogenesis of IPF. This study aimed to elucidate the characteristics of small airway epithelial dysfunction in patients with IPF and explore novel therapeutic interventions to impede IPF progression by targeting the dysfunctional small airways.

Prevalence of metal implants among US adults aged 40 years and older.

Metal implants are commonly used in clinical practice. However, little is known regarding the prevalence of metal implants. Therefore, this study aimed to evaluate the prevalence of metal implants in the United States (US) among individuals aged ≥ 40 years.

Road properties of cement-phosphogypsum-red clay under dry and wet cycles.

In this paper, the road performance and mechanism of cement-phosphogypsum-red clay (CPRC) under dry and wet cycling were systematically investigated using 5% cement as curing agent, the mass ratio of phosphogypsum: red clay = 1:1, and 5% SCA-2 as water stabilizer. The road performance of dry and wet cycle mix was verified with the National Highway G210 Duyun Yangan to Yingshan Highway Reconstruction and Expansion Project as a test road to provide a scientific basis for the application of cement-phosphogypsum-red clay on roads. The results show that the cement-phosphogypsum-red clay unconfined compressive strength decreases with the increase of the number of wet and dry cycles, with a larger decay in the first three times and leveling off thereafter.

Elevated Serum Direct Bilirubin and Smoking Status Are Prognostic Factors for EGFR-Mutated Non-Small Cell Lung Cancer Patients Receiving Tyrosine Kinase Inhibitor Therapy.

Lung cancer is one of the leading causes of cancer-related death worldwide [...

Conicity index and sex differences in relation to carotid plaque instability in Chinese community residents: Conicity index and carotid plaque.

Objectives: The conicity index (CI), an anthropometric parameter of visceral obesity, has exhibited a strong correlation with the incidence of atherosclerosis and cardiovascular events. This study aimed to explore the relationship between CI and carotid plaque instability, as well as the potential differences between sexes in this context.

Methods: Our cross-sectional study included a total of 44,248 participants from the China Stroke High-risk Population Screening and Intervention Program (CSHPSIP), all of whom underwent carotid ultrasound for the evaluation of carotid plaque.

Incidental findings in preoperative computed tomography images of robotic-assisted total joint replacement: a multi-center retrospective study.

Background: The majority of robot-assisted total joint arthroplasties necessitate preoperative computed tomography (CT) scans. Incidental findings in these CT scans can introduce complexity into clinical treatment decisions. Consequently, this study sought to document the nature and frequency of incidental findings identified in preoperative CT imaging conducted for robot-assisted total joint arthroplasty, along with their impact on planned surgical procedures.

Association between work-related physical activity and mortality among US Cohort.

Purpose: The benefits of leisure-time physical activity (LTPA) in reducing mortality are well-known, while the relationship between work-related physical activity (WRPA) and mortality remains controversial. The study aimed to investigate the association between WRPA and mortality, and to determine whether LTPA should be recommended in a population with a high level of WRPA.

Method: Data were obtained from a sample of adults aged 20-80 years included in the National Health and Nutrition Examination Survey (NHANES) cohort from 2007 to 2018.

Robust Sodium Carboxymethyl Cellulose-Based Neuromorphic Device with High Biocompatibility Engineered through Molecular Polarization for the Emulation of Learning Behaviors in the Human Brain.

Sodium carboxymethyl cellulose (CMC-Na), derived from natural cellulose and frequently employed as a biocompatible coating, thus renders it an ideal component for the construction of highly biocompatible neuromorphic devices aimed at biomachine interfaces. Here, an array of Mo/CMC-Na/ITO neuromorphic devices is fabricated, with CMC-Na serving as the functional layer. The devices exhibit capabilities to emulate various synaptic learning rules and demonstrate high endurance performance among biomaterial-based electronics, achieving stability over 2 × 10 pulses.

Application of multi-modal temporal neural network based on enhanced sparrow optimization in lithium battery life prediction.

This paper introduces the DeNet-Mamba-DC-SCSSA network, an advanced solution for predicting the Remaining Useful Life (RUL) of lithium-ion batteries, crucial for the safety and efficiency management of electric vehicles. Combining the robust Denoising Enhancement Network (DeNet), the Improved Sparrow Optimization Algorithm (SCSSA), the adept Mamba time-series model, and the proficient Dilated Convolution (DC), this model excels in precise noise handling and sophisticated feature extraction. DeNet diligently refines input data, mitigating noise interference, while Mamba skillfully captures sequential intricacies.