Characterisation and identification of chemical constituents in aqueous extract of Fomes officinalis Ames based on ultrahigh-performance liquid chromatography tandem quadrupole-Orbitrap high-resolution mass spectrometry.

Introduction: Fungal species are an attractive resource for physiologically functional food and drug precursor. Fomes officinalis Ames, a medicinal fungus, is traditionally used as a folk medicine in traditional Chinese medicine prescription for the therapy of cough and asthma. The water-soluble substances in Chinese herbal medicines are likely to play an important physiological function.

Piptolinic acids F-J, five new lanostane-type triterpenoids from .

Five new lanostane-type triterpenoids, named piptolinic acids F - J (-), as well as seven known analogues (-), were isolated from methanolic extract of the fruiting bodies of . Compounds - were 24-methyl-lanostane triterpenoids, while compound was a 3,4--lanostane derivative. Their structures were established on the basis of extensive spectroscopic analysis (1D, 2D NMR, and HRESIMS).

Cytotoxic lanostane triterpenoids from the fruiting bodies of Piptoporus betulinus.

Chemical investigation of a bioactive methanolic extract of the fruiting bodies of Piptoporus betulinus led to the isolation of five previously undescribed lanostane triterpenoids named piptolinic acids A-E, as well as five known lanostane triterpenoids. Their structures were elucidated on the basis of 1D and 2D NMR spectroscopic and HRESIMS analysis. Piptolinic acid A with an unusual moiety (3-hydroxy-4-methoxycarbonyl-3-methylbutyryloxy) at C-3 exhibited comparable cytotoxic activity against human promyelocytic leukemia cell line HL-60 (IC = 1.