Publications by authors named "Zeynep S Yunt"
Article Synopsis
- Researchers introduce a new method called Plasma Membrane-BioID to effectively label and isolate plasma membrane proteins from mammalian cells without additional labeling steps.
- This technique uses a lipid-modified enzyme (MyrPalm BirA*) that targets the inner leaflet of the plasma membrane to biotinylate the proteins.
- By combining this method with traditional sucrose density gradient centrifugation, they achieve highly pure plasma membrane components suitable for thorough mass spectrometry analysis, improving the study of integral membrane proteins.
The benastatins, pradimicins, fredericamycins, and members of the griseorhodin/rubromycin family represent a structurally and functionally diverse group of long-chain polyphenols from actinomycetes. Comparison of their biosynthetic gene clusters (ben, prm, fdm, grh, rub) revealed that all loci harbor genes coding for a similar, yet uncharacterized, type of ketoreductases. In a phylogenetic survey of representative KRs involved in type II PKS systems, we found that it is generally possible to deduce the KR regiospecificity (C-9, C-15, C17) from the amino acid sequence and thus to predict the nature of the aromatic polyketide (e.
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