m-Aminophenylacetylene shortened the eggsac-carrying stages in the wolf spider through enhancing the synthesis of prostaglandins.

Spiders are important predatory enemies that control a range of insect pests in the rice ecosystem. m-Aminophenylacetylene (m-A) has been demonstrated to induce maternal care behaviors in Pardosa pseudoannulata, protecting young spiders and promoting population growth. Here the roles of m-A on other reproduction stages were evaluated by injecting m-A into virgin and postreproductive female spiders.

Peptide neurotoxins affecting insect voltage-gated calcium channels and possessing insecticidal toxicity: Two ω-Atypitoxins from Calommata signata.

Spider peptide toxins, as potent insecticides, distinguish them from current insecticides, and thus expand the range of viable molecular targets of insecticides in insect pests. This study reports the isolation of two neurotoxic peptides, ω-Atyptoxin-Cs2a (Cs2a) and ω-Atyptoxin-Cs2b (Cs2b), from the venom gland of the burrowing spider Calommata signata. These peptides exhibit strong insecticidal activity against two insect pests with agricultural importance, Nilaparvata lugens and Spodoptera frugiperda.

Research on the Assembly of Square Parts on Circular Production Lines Using Admittance Control for Six-DOF Robots.

In circular production lines, issues such as positional errors, manufacturing errors at different square groove workstations, and the accumulation of errors during continuous assembly reduce the assembly success rate and limit efficiency. To address these challenges in assembling square groove parts, this study focuses on the jamming and wedging problems encountered during the assembly of square grooves and square parts. It analyzes typical assembly hole searching contact situations and proposes corresponding strategies.

Discovery of Novel Neonicotinoids with Conjugated Diene Enabled by Cascade Allylation/Isomerization Synthetic Methodology.

As one of the most significant insecticides, neonicotinoids have played a pivotal role in crop protection and public sanitation. However, the high resistance and bee toxicity of neonicotinoid insecticides have attracted considerable attention. Herein, a series of neonicotinoid compounds with conjugated diene moieties were synthesized through the cascade allylation/isomerization reaction.

The cross-resistance to etofenprox in Nilaparvata lugens with a high adaptability to resistant rice variety IR56.

Background: The application of resistant rice varieties and insecticides represents two crucial strategies for managing the brown planthopper (BPH), Nilaparvata lugens (Stål). Insects often employ similar detoxification mechanisms to metabolize plant secondary metabolites and insecticides, which poses a potential risk that BPH population adapted to resistant rice may also obtain resistance to some insecticides.

Results: Here in a BPH population (R-IR56) that has adapted to the resistant rice variety IR56 through continuous selection, the moderate resistance to etofenprox was observed.

Study on the Effect of Phillyrin on In Vivo and In Vitro.

As a zoonotic pathogen, serotype 2 (SS2) can cause severe diseases in both pigs and humans, and develop resistance to antibiotics. Plant natural compounds are regarded as promising alternatives to conventional antibiotics. Phillyrin is the major bioactive components of Chinese herbal medicine Forsythia suspensa.

A novel self-assembling peptide nanofiber hydrogel with glucagon-like peptide-1 functionality enhances islet survival to improve islet transplantation outcome in diabetes treatment.

Islet transplantation is a promising therapy for diabetes, yet the limited survival and functionality of transplanted islet grafts hinder optimal outcomes. Glucagon-like peptide-1 (GLP-1), an endogenous hormone, has shown potential to enhance islet survival and function; however, its systemic administration can result in poor localization and undesirable side effects. To address these challenges, we developed a novel peptide-based nanofiber hydrogel incorporating GLP-1 functionality for localized delivery.

Roles of spermary-specific carboxylesterases in Nilaparvata lugens reproduction: Opposite between insecticide-induced upregulation and resistance-associated overexpression.

Seminal fluid proteins (SFPs) in insect males are critical for reproduction, being transported into female ovary. In Nilaparvata lugens, a significant rice pest, seven spermary-specific carboxylesterases (CarEs) were found abundantly in SFPs, with over-expression in males of an imidacloprid-resistant (RES) strain compared to a susceptible (SUS) strain. This study aimed to evaluate roles of spermary-specific CarEs in N.

Omeprazole and its analogs exhibit insecticidal potencies as inhibitors of insect choline acetyltransferase.

Choline acetyltransferase (ChAT) is crucial for acetylcholine synthesis and regulates diverse functions in numerous biological processes. Omeprazole, an inhibitor on human ChAT, was evaluated here on insect ChAT as a potential inhibitor, as well as its insecticidal potency on Nilaparvata lugens, a major insect pest on rice. The evaluation also included omeprazole analogs and α-NETA, in order to explore a superior leading compound targeting on insect ChAT.

Overexpression of ace2 compensating for the acetylcholinesterase activity loss from ace1 mutations accelerated the development of eggs and early nymphs in Nilaparvata lugens.

Acetylcholinesterase (AChE) guarantees the acetylcholine signal in insect central nervous system, and is the target of organophosphorus and carbamate insecticides, towards which resistance was often reported due to AChE mutations. In Nilaparvata lugens, a major pest on rice, two mutations (G119S and F331C) were detected in AChE1 in a chlorpyrifos-resistant (CHL) strain. The double mutations in AChE1 reduced total AChE activity in metabolizing acetylcholine, such as low protein stability, substrate affinity and catalytic efficiency, which needed compensation in a special way.

Rosmarinic acid-mediated downregulation of RIG-I and p62 in microglia confers resistance to Japanese encephalitis virus-induced inflammation.

Background: Japanese encephalitis virus (JEV) is a mosquito-borne zoonotic pathogen that causes encephalitis in humans and reproductive failure in pigs. The transmission of JEV between humans and animals poses a significant public health threat and results in substantial economic losses. Excessive inflammation in the central nervous system of JEV-infected patients is a major cause of mortality and disability.

Pesticides enhance maternal care in the wolf spider by inhibiting HSD3B, an enzyme for progesterone synthesis.

Natural enemies are essential for the integrated pest management (IPM) strategy in insect pest control. However, chemical pesticides remain the primary method for insect pest control, diminishing natural enemies and causing environmental contamination. The wolf spider (Pardosa pseudoannulata), a major enemy in agro-ecosystem, exhibits maternal care (MC) behaviors such as eggsac-carrying and pulli-carrying, which are crucial for population growth.

Akt-FoxO signaling drives co-adaptation to insecticide and host plant stresses in an herbivorous insect.

Introduction: Ongoing interactions between host and herbivorous insect trigger a co-evolutionary arms race. Genetic diversity within insects facilitates their adaptation to phytochemicals and their derivatives, including plant-derived insecticides. Cytochrome P450s play important roles in metabolizing phytochemicals and insecticides, due to their diversity and almost perfect evolution.

Rational design and discovery of novel hydrazide derivatives as potent succinate dehydrogenase inhibitors inspired by natural d/l-camphor.

Background: Succinate dehydrogenase inhibitors (SDHIs) have rapidly become one of the fastest-growing categories of fungicides used against plant pathogenic fungi. Recent research advancements have emphasized that structural modifications of SDHIs using naturally sourced scaffolds represent an innovative strategy for developing new, highly effective, broad-spectrum fungicides. A novel series of d/l-camphorhydrazide derivatives potentially targeting fungal succinate dehydrogenase (SDH) were designed, synthesized and evaluated for their antifungal effects against Rhizoctonia solani, Fusarium graminearum, Valsa mali and Botrytis cinerea.

Glyphosate-based herbicides reduced overwintering population and reproduction of agrobiont spiders.

Spiders are important in ecosystem and serve as predators in the biological control of pest insects in agroecosystem, where they encounter various harsh challenges including pesticides and low temperature in winter. Glyphosate-based herbicides (GBH) are widely and frequently applied to diminish weeds, exposing spiders a disturbed habitat, especially to overwintering spiders. We conducted a study combining field surveys and lab assays, to assess the effects of a GBH on the overwintering of the agrobiont wolf spider, Pardosa pseudoannulata.

Knockdown of Fzr inhibited the growth of Nilaparvata lugens by blocking endocycle.

Background: The endocycle can generate cells referred to as 'polyploid'. Fizzy-related protein (Fzr) plays an important role in driving the mitosis-to-endocycle transition. The brown planthopper (BPH), Nilaparvata lugens (Stål), a serious insect pest, feeds exclusively on rice.

CYP4CE1 Metabolized Nitenpyram through Two Types of Oxidation Reaction, Hydroxylation, and N-Demethylation.

Nitenpyram, taking the place of imidacloprid, is a widely used neonicotinoid insecticide to control in Asia. Two P450s, CYP4CE1 and CYP6ER1, are key factors in the metabolic resistance against nitenpyram and imidacloprid. In this study, we found that expression was strongly associated with nitenpyram resistance in 8 field-collected populations, whereas expression correlated with imidacloprid resistance.