Frequency of respiratory virus-associated infection among children and adolescents from a tertiary-care hospital in Mexico City.

Acute respiratory infections (ARIs) are a major cause of morbidity and mortality among children. The causative pathogens show geographic and seasonal variations. We retrospectively evaluated the frequency and seasonality of respiratory pathogens in children and adolescents (age: 0-19 years) with ARIs treated between January 1, 2021, and March 31, 2022, at a single center in Mexico.

Recombinant BL21 with LngA Variants from ETEC E9034A Promotes Adherence to HT-29 Cells.

Unlabelled: The CS21 pilus produced by enterotoxigenic (ETEC) is involved in adherence to HT-29 intestinal cells. The CS21 pilus assembles proteins encoded by 14 genes clustered into the operon.

Aim: This study aimed to determine whether .

The Assembly of Flagella in Enteropathogenic Requires the Presence of a Functional Type III Secretion System.

In enteropathogenic (EPEC), the production of flagella and the type III secretion system (T3SS) is activated in the presence of host cultured epithelial cells. The goal of this study was to investigate the relationship between expression of flagella and the T3SS. Mutants deficient in assembling T3SS basal and translocon components (Δ, Δ, Δ, Δ, Δ, and Δ), and in secreting effector molecules (Δ and Δ) were tested for flagella production under several growth conditions.

Clinical Characteristics of Coronavirus Disease (COVID-19) in Mexican Children and Adolescents.

Background: We analyzed the demographic, clinical, and diagnostic data of children and adolescents in Mexico, from the first case of coronavirus disease (COVID-19) to 28 February 2022.

Methods: Using the open databases of the Ministry of Health and a tertiary pediatric hospital, we obtained demographic and clinical data from the beginning of the COVID-19 pandemic until 28 February 2022. In addition, quantitative reverse-transcription polymerase chain reaction outputs were used to determine the viral load, and structural protein-based serology was performed to evaluate IgG antibody levels.

The EcpD Tip Adhesin of the Common Pilus Mediates Binding of Enteropathogenic to Extracellular Matrix Proteins.

The attachment of enteropathogenic (EPEC) to intestinal epithelial cells is facilitated by several adhesins; however, the individual host-cell receptors for pili-mediated adherence have not been fully characterized. In this study, we evaluated the hypothesis that the common pilus (ECP) tip adhesin protein EcpD mediates attachment of EPEC to several extracellular matrix (ECM) glycoproteins (fibronectin, laminin, collagens I and IV, and mucin). We found that the Δ mutant, which lacks production of the EcpA filament but retains EcpD on the surface, adhered to these glycoproteins below the wild-type levels, while the Δ mutant, which does not display EcpA or EcpD, bound significantly less to these host glycoproteins.

FoxR is an AraC-like transcriptional regulator of ferrioxamine uptake in Salmonella enterica.

Salmonella enterica spp. produce siderophores to bind iron with high affinity and can also use three xenosiderophores secreted by other microorganisms, ferrichrome, coprogen, and ferrioxamine. Here we focused on FoxA, a TonB-dependent transporter of ferrioxamines.

The Fis Nucleoid Protein Negatively Regulates the Phase Variation Switch of the Type 1 Pilus Operon in Enteropathogenic .

In the expression of type 1 pili (T1P) is determined by the site-specific inversion of the ON-OFF switch located immediately upstream of major fimbrial subunit gene . Here we investigated the role of virulence (Ler, GrlR, and GrlA) and global regulators (H-NS, IHF, and Fis) in the regulation of the switch in the human enteropathogenic (EPEC) O127:H6 strain E2348/69. This strain does not produce detectable T1P and PCR analysis of the switch confirmed that it is locked in the OFF orientation.

The Transcription of Flagella of Enteropathogenic O127:H6 Is Activated in Response to Environmental and Nutritional Signals.

The flagella of enteropathogenic (EPEC) O127:H6 E2348/69 mediate adherence to host proteins and epithelial cells. What environmental and nutritional signals trigger or down-regulate flagella expression in EPEC are largely unknown. In this study, we analyzed the influence of pH, oxygen tension, cationic and anionic salts (including bile salt), carbon and nitrogen sources, and catecholamines on the expression of the flagellin gene ( of E2348/69.

Intracellular niche-specific profiling reveals transcriptional adaptations required for the cytosolic lifestyle of Salmonella enterica.

Salmonella enterica serovar Typhimurium (S. Typhimurium) is a zoonotic pathogen that causes diarrheal disease in humans and animals. During salmonellosis, S.

Coliform bacteria in San Pedro Lake, western Mexico.

Urbanization, livestock activities, and rainfall are factors that contribute to the contamination of inland water. This study aimed to determine the spatial and temporal variability of total coliforms (TCs) and fecal coliforms (FCs) in the surface water of San Pedro Lake as well as the gills and skin of Nile tilapia (Oreochromis niloticus) cultivated in the lake. The study consisted of seasonal sampling during an annual cycle.

Tracking Bioluminescent ETEC during BALB/c Mouse Colonization.

Enterotoxigenic (ETEC) is a leading cause of diarrhea worldwide. Adhesion to the human intestinal tract is crucial for colonization. ETEC adhesive structures have been extensively studied; however, colonization dynamics remain uncharacterized.

Genome Sequence of Enterotoxigenic Strain FMU073332.

Enterotoxigenic (ETEC) is an important cause of bacterial diarrheal illness, affecting practically every population worldwide, and was estimated to cause 120,800 deaths in 2010. Here, we report the genome sequence of ETEC strain FMU073332, isolated from a 25-month-old girl from Tlaltizapán, Morelos, México.

Corrigendum: Effects of Mutations on LngA Expression, Processing, and CS21 Assembly in Enterotoxigenic E9034A.

[This corrects the article on p. 1201 in vol. 7, PMID: 27536289.

Dimeric and Trimeric Fusion Proteins Generated with Fimbrial Adhesins of Uropathogenic .

Urinary tract infections (UTIs) are associated with high rates of morbidity and mortality worldwide, and uropathogenic (UPEC) is the main etiologic agent. Fimbriae assembled on the bacterial surface are essential for adhesion to the urinary tract epithelium. In this study, the FimH, CsgA, and PapG adhesins were fused to generate biomolecules for use as potential target vaccines against UTIs.

Effects of lng Mutations on LngA Expression, Processing, and CS21 Assembly in Enterotoxigenic Escherichia coli E9034A.

Enterotoxigenic Escherichia coli (ETEC) is a major cause of morbidity in children under 5 years of age in low- and middle-income countries and a leading cause of traveler's diarrhea worldwide. The ability of ETEC to colonize the intestinal epithelium is mediated by fimbrial adhesins, such as CS21 (Longus). This adhesin is a type IVb pilus involved in adherence to intestinal cells in vitro and bacterial self-aggregation.