Three-dimensional structural alignment based discovery and molecular basis of AtoB, catalyzing linear tetracyclic formation.

Enzymes from the nuclear transport factor 2-like (NTF2-like) superfamily represent a rare group of biocatalysts with diverse catalytic functions facilitating intriguing skeleton formations. However, most proteins of this family remain enigmatic and await further elucidation. In this study, a combination of protein structural alignment with clustering analysis uncovers a new aldolase, AtoB, belonging to the NTF2-like superfamily.

Dynamic DNA methylation modifications in the cold stress response of cassava.

Cassava, a crucial tropical crop, faces challenges from cold stress, necessitating an exploration of its molecular response. Here, we investigated the role of DNA methylation in moderating the response to moderate cold stress (10 °C) in cassava. Using whole-genome bisulfite sequencing, we examined DNA methylation patterns in leaf blades and petioles under control conditions, 5 h, and 48 h of cold stress.

Brominated Depsidones with Antibacterial Effects from a Deep-Sea-Derived Fungus sp.

Eleven new brominated depsidones, namely spiromastixones U-Z5 (-) along with five known analogues (-), were isolated from a deep-sea-derived fungus sp. through the addition of sodium bromide during fermentation. Their structures were elucidated by extensive analysis of the spectroscopic data including high-resolution MS and 1D and 2D NMR data.

A Prediction Model of Defecation Based on BP Neural Network and Bowel Sound Signal Features.

(1) Background: Incontinence and its complications pose great difficulties in the care of the disabled. Currently, invasive incontinence monitoring methods are too invasive, expensive, and bulky to be widely used. Compared with previous methods, bowel sound monitoring is the most commonly used non-invasive monitoring method for intestinal diseases and may even provide clinical support for doctors.