Roles of Response Regulators in the Two-Component System in the Formation of Stress Tolerance, Motility and Biofilm in Enteritidis.

Two-component systems (TCS) of serovar Enteritidis are composed of a histidine kinase and a response regulator (RR) and represent a critical mechanism by which bacteria develop resistance to environmental stress. Here, we characterized the functions of RRs in TCS in the formation of stress tolerance, motility and biofilm using twenty-six . Enteritidis RR-encoding gene deletion mutants.

Dissemination of IncC plasmids in Salmonella enterica serovar Thompson recovered from seafood and human diarrheic patients in China.

Salmonella Thompson is a prevalent foodborne pathogen and a major threat to food safety and public health. This study aims to reveal the dissemination mechanism of S. Thompson with co-resistance to ceftriaxone and ciprofloxacin.

BolA affects the biofilm formation ability, outer membrane permeability and virulence, thus is required for the adaptability of Salmonella enterica serotype Typhimurium to the harsh survival environment.

Salmonella enterica serotype Typhimurium, an important foodborne pathogen with high adaptability to the host's internal and external survival environment, seriously threatens public health. Therefore, to understand the mechanism underlying the high adaptability, this study investigated the transcription factor BolA by constructing BolA deletion strain 269△BolA, complemented strain 269BolAR and overexpression strain 269BolA+ based on WT269. BolA significantly inhibited motility; at 6 h, the BolA overexpression strain (269BolA+) showed 91.

Genomic Characterization of Salmonella enterica serovar Weltevreden Associated with Human Diarrhea.

Salmonella Weltevreden is an emerging pathogen associated with human diarrhea, and knowledge of the genomics and epidemiology of this serovar is still limited. In this study, we performed whole-genome sequencing of 96 Weltevreden isolates recovered from diarrheal patients and 62 isolates from food animals in China between 2006 and 2017. Together, with an additional 199 genome sequences of .

Ethanol at Subinhibitory Concentrations Enhances Biofilm Formation in Enteritidis.

The survival of Enteritidis in the food chain is relevant to its biofilm formation capacity, which is influenced by suboptimal environmental conditions. Here, biofilm formation pattern of this bacterium was assessed in the presence of ethanol at sub-minimal inhibitory concentrations (sub-MICs) by microtiter plate assays, cell characteristic analyses, and gene expression tests. It was observed that ethanol at subinhibitory concentrations (1/4 MIC, 2.

International Spread of Tet(X4)-Producing Isolates.

Tigecycline resistance in bacteria has become a significant threat to food safety and public health, where the development of which is attributed to plasmid-mediated (X4) genes. In this study, the genomes of 613 (X4)-producing () isolates, available from public databases, are evaluated to determine their international prevalence and molecular characterization. These isolates have been disseminated in 12 countries across Asia and Europe.

Antimicrobial resistance and molecular characterization of Salmonella enterica serovar Corvallis isolated from human patients and animal source foods in China.

In this study, 205 Salmonella enterica serovar Corvallis strains were obtained from humans and foods from Guangdong, Guangxi, and Shanghai in China from 2009 to 2017 to assess drug resistance and molecular epidemiology. These isolates displayed high rates of resistance to sulfisoxazole (94.15%) and tetracycline (77.

A rapid novel visualized loop-mediated isothermal amplification method for Salmonella detection targeting at fimW gene.

Salmonella infection causes huge losses in the poultry industry worldwide. With the aim to prevent infectious diseases caused by Salmonella and to achieve rapid visualized Salmonella detection in poultry production, we used cresol red as an indicator to develop a novel visualized loop-mediated isothermal amplification method that targets the Salmonella fimW gene firstly in related field. The detection limit was 7.

Plasmid-Encoded Gene That Confers High-Level Carbapenem Resistance in Typhimurium of Pork Origin.

Purpose: Carbapenem resistance is rarely reported in Typhimurium, especially from a food origin. Here, we report a plasmid-mediated mobile carbapenem-resistant gene in Typhimurium isolated from pork in Shanghai, China in 2016.

Patients And Methods: In July 2016, the .

Prevalence, Antimicrobial Resistance, Virulence Genes and Genetic Diversity of Isolated from Retail Duck Meat in Southern China.

is an important cause of foodborne diseases. This study was undertaken to investigate the prevalence, serotype distribution, antimicrobial resistance, virulence genes, and genetic diversity of isolates recovered from fresh duck meat obtained from retail markets in Southern China. In total, 365 samples of fresh duck meat were collected from retail markets in six different cities of Guangdong Province between May 2017 and April 2019.

Ciprofloxacin-Resistant Serovar Kentucky ST198 in Broiler Chicken Supply Chain and Patients, China, 2010-2016.

serovar Kentucky (. Kentucky) sequence type 198 has emerged as a global zoonotic pathogen. We explored serovar Kentucky ST198 samples from the broiler chicken supply chain and patients between 2010 and 2016.

Rapid Emergence of Florfenicol-Resistant Invasive Non-Typhoidal in China: A Potential Threat to Public Health.

Infection caused by invasive occurs when bacteria, which normally cause diarrhea, enter the bloodstream and spread through the body. We report the dramatic increase in florfenicol-resistant invasive non-typhoidal (iNTS) in China between 2007 and 2016. Of the 186 iNTS strains isolated during the study period, 34 were florfenicol resistant, most of which harbored known resistance genes.

Molecular epidemiology and antimicrobial resistance of invasive non-typhoidal in China, 2007-2016.

Purpose: Human infections caused by invasive non-typhoidal (iNTS) are highly prevalent worldwide. However, data for such infections in China are scarce. This study reports the epidemiology of iNTS in China.

High-levels of resistance to quinolone and cephalosporin antibiotics in MDR-ACSSuT Salmonella enterica serovar Enteritidis mainly isolated from patients and foods in Shanghai, China.

In this study, 2887 Salmonella strains were mainly obtained from patients and foods in Shanghai from 2006 to 2014 in order to assess the susceptibility to 16 antibiotics. Among them, 3.8% (110/2887) S.

Antimicrobial Susceptibility and Molecular Typing of Salmonella Senftenberg Isolated from Humans and Other Sources in Shanghai, China, 2005 to 2011.

Salmonella Senftenberg is an important nontyphoidal Salmonella serovar that causes gastrointestinal disease worldwide. In total, 130 Salmonella Senftenberg strains obtained from humans, food, and the environment in Shanghai, People's Republic of China, were characterized for antimicrobial susceptibility and subjected to molecular typing. Our findings indicated that most (96 of 130, 73.