Tannins and copper sulphate as antimicrobial agents to prevent contamination of seedling culture for restoration purposes.

Seed-based restoration methods are increasingly recognized as a relevant tool contributing to halt and reverse the loss of seagrass meadows while providing genetic and evolutionary benefit for the conservation of these habitats. protocols aimed at maximizing the survival of plantlets obtained from seeds in cultivation systems are therefore required. Previous trials of seedling culture of , the dominant seagrass of the Mediterranean Sea, recorded up to 40% loss due to mould development.

Exploring class III cellobiose dehydrogenase: sequence analysis and optimized recombinant expression.

Background: Cellobiose dehydrogenase (CDH) is an extracellular fungal oxidoreductase with multiple functions in plant biomass degradation. Its primary function as an auxiliary enzyme of lytic polysaccharide monooxygenase (LPMO) facilitates the efficient depolymerization of cellulose, hemicelluloses and other carbohydrate-based polymers. The synergistic action of CDH and LPMO that supports biomass-degrading hydrolases holds significant promise to harness renewable resources for the production of biofuels, chemicals, and modified materials in an environmentally sustainable manner.

Exploring priming strategies to improve stress resilience of Posidonia oceanica seedlings.

Seagrasses' ability to store information after exposure to stress (i.e. stress memory) and to better respond to further stress (i.

Continuous photometric activity assays for lytic polysaccharide monooxygenase-Critical assessment and practical considerations.

Lytic polysaccharide monooxygenase (LPMO) is a monocopper-dependent enzyme that cleaves glycosidic bonds by using an oxidative mechanism. In nature, they act in concert with cellobiohydrolases to facilitate the efficient degradation of lignocellulosic biomass. After more than a decade of LPMO research, it has become evident that LPMOs are abundant in all domains of life and fulfill a diverse range of biological functions.

Biological adhesion in seagrasses: The role of substrate roughness in Posidonia oceanica (L.) Delile seedling anchorage via adhesive root hairs.

Seagrasses are marine flowering plants that developed several adaptive traits for living in submerged waters. Among this group, Posidonia oceanica (L.) Delile is the dominant species of the Mediterranean Sea, forming persistent meadows that provide valuable ecosystem services to human communities.

The perfect microsite: How to maximize Posidonia oceanica seedling settlement success for restoration purposes using ecological knowledge.

The use of sexual propagules to restore seagrass meadows has raised increasing attention in the last years as seed-based strategies avoid impacts on donor beds while preserving genetic diversity in restored populations. However, the availability of suitable microsites for seedling establishment at transplantation locations is crucial in order to achieve positive outcome of restoration actions. In this study we develop ad-hoc holders that act as optimal microsites for Posidonia oceanica seedling establishment.

Microplastics impair the feeding performance of a Mediterranean habitat-forming coral.

The impact of plastic debris, and in particular of microplastics (here referred as particles smaller than 5 mm) on aquatic environments has now become a topic of raising concern. Microplastics are particularly abundant in the Mediterranean Sea, potentially exerting substantial pressures on marine organisms at different levels of organization. Ingestion of microplastics has been observed in a large number of marine species.