GenoBaits®WheatplusEE: a targeted capture sequencing panel for quick and accurate identification of wheat-Thinopyrum derivatives.

A total of 90,000 capture probes derived from wheat and Thinopyrum elongatum were integrated into one chip, which served as an economical genotype for explorating Thinopyrumspecies and their derivatives. Thinopyrum species play a crucial role as a source of new genetic variations for enhancing wheat traits, including resistance to both abiotic and biotic factors. Accurate identification of exogenous chromosome(s) or chromosome segments or genes is essential following the introduction of alien genetic material into wheat, but this task remains challenging.

Cytogenetic identification and molecular marker development of a novel wheat- 4Ns(4D) alien disomic substitution line with resistance to stripe rust and Fusarium head blight.

(Trin.) Pilg. (2 = 4 = 28, NsNsXmXm) potentially harbours useful genes that might contribute to the improvement of wheat.

Identification and DNA Marker Development for a Wheat- 2Ns (2D) Disomic Chromosome Substitution.

(2 = 4 = 28, NsNsXmXm), a wild relative of common wheat ( L.), carries numerous loci which could potentially be used in wheat improvement. In this study, line 17DM48 was isolated from the progeny of a wheat and hybrid.

Chromosome karyotype and stability of new synthetic hexaploid wheat.

Synthetic hexaploid wheat offers breeders ready access to potentially novel genetic variation in wild ancestral species. In this study, we crossed MY3478 (2 = 4 = 28, AABB) as the maternal parent with the stripe rust-resistant SY41 (2 = 2 = 14, DD) as the paternal parent to construct the new hexaploid wheat line NA0928 through natural allopolyploidization. Agronomic traits and the cytology of the S-S generations of NA0928 were analyzed.

Gene co-expression network analysis provides a novel insight into the dynamic response of wheat to powdery mildew stress.

Powdery mildew ( f. sp. , ()) is an important worldwide fungal foliar disease of wheat () responsible for severe yield losses.

Characterization and Evaluation of Resistance to Powdery Mildew of Wheat- Roth 7M (7A) Alien Disomic Substitution Line W16998.

Roth has been used as a donor of disease-resistance genes, to enrich the gene pool for wheat () improvement through distant hybridization. In this study, the wheat- alien disomic substitution line W16998 was obtained from the BCF progeny of a cross between the common wheat 'Chinese Spring' (CS) and Roth (serial number: SY159//CS). This line was identified using cytogenetic techniques, analysis of genomic in situ hybridization (GISH), functional molecular markers (Expressed sequence tag-sequence-tagged site (EST-STS) and PCR-based landmark unique gene (PLUG), fluorescence in situ hybridization (FISH), sequential fluorescence in situ hybridization-genomic in situ hybridization (sequential FISH-GISH), and assessment of agronomic traits and powdery mildew resistance.

Isolation and molecular cytogenetic characterization of a wheat - Leymus mollis double monosomic addition line and its progenies with resistance to stripe rust.

A common wheat - Leymus mollis (2n = 4x = 28, NsNsXmXm) double monosomic addition line, M11003-4-3-8/13/15 (2n = 44 = 42T.a + L.m2 + L.

Molecular cytogenetics identification of a wheat - Leymus mollis double disomic addition line with stripe rust resistance.

Leymus mollis (Trin.) Pilg. (2n = 4x = 28, NsNsXmXm) possesses a number of valuable genes against biotic and abiotic stress, which could be transferred into common wheat background for wheat improvement.

Development and Molecular Cytogenetic Identification of a Novel Wheat-Leymus mollis Lm#7Ns (7D) Disomic Substitution Line with Stripe Rust Resistance.

Leymus mollis (2n = 4x = 28, NsNsXmXm) possesses novel and important genes for resistance against multi-fungal diseases. The development of new wheat-L. mollis introgression lines is of great significance for wheat disease resistance breeding.

Chromosome constitution and origin analysis in three derivatives of Triticum aestivum--Leymus mollis by molecular cytogenetic identification.

Leymus mollis (2n = 4x = 28, NsNsXmXm) is an important tetraploid species in Leymus (Poaceae: Triticeae) and a useful genetic resource for wheat breeding because of the stress tolerance and disease resistance of this species. The development of Triticum aestivum (common wheat) - L. mollis derivatives with desirable genes will provide valuable bridge materials for wheat improvement, especially regarding powdery mildew resistance genes, which are rarely documented in L.