Integrated bioinformatics investigation of adenylyl cyclase family co-expression network in bladder cancer followed by preliminary validation of member 2 () in tumorigenesis and prognosis.

Background: The adenylyl cyclase () gene family encodes enzymes responsible for the synthesis of cyclic adenosine monophosphate (cAMP) from adenosine triphosphate (ATP), which comprises nine transmembrane isoforms (). Although correlate with intracellular signalling and tumorigenesis in different malignancies, their roles in bladder cancer remain unclear.

Methods: Utilizing the bladder urothelial carcinoma (BLCA) dataset from The Cancer Genome Atlas (TCGA), we employed the R package 'limma' to identify differential genes.

Fascin 1 promoted the growth and migration of non-small cell lung cancer cells by activating YAP/TEAD signaling.

Fascin 1 (Fascin actin-bundling protein 1) is an actin-binding protein. Although several studies have reported the dysregulation of Fascin 1 in non-small cell lung cancer (NSCLC), its functions in the progression of NSCLC and the related molecular mechanism were not fully understood. In this study, the expression of Fascin 1 in NSCLC tissues was determined using quantitative PCR (qPCR), and the roles of Fascin 1 in the progression of NSCLC were investigated.

Geological disaster survey based on Curvelet transform with borehole Ground Penetrating Radar in Tonglushan old mine site.

Tonglushan old mine site located in Huangshi City, China, is very famous in the world. However, some of the ruins had suffered from geological disasters such as local deformation, surface cracking, in recent years. Structural abnormalities of rock-mass in deep underground were surveyed with borehole ground penetrating radar (GPR) to find out whether there were any mined galleries or mined-out areas below the ruins.

Direct fabrication of homogeneous microfluidic channels embedded in fused silica using a femtosecond laser.

We demonstrate direct fabrication of homogeneous microfluidic channels embedded in fused silica by femtosecond laser direct writing, followed by wet chemical etching and glass drawing. In addition, the glass drawing process significantly reduces the inner surface roughness of the fabricated channels, and centimeter-level microfluidic channels with an aspect ratio above 1000 can be realized.

Electro-optic integration of embedded electrodes and waveguides in LiNbO3 using a femtosecond laser.

We describe the fabrication of a Mach-Zehnder optical modulator in LiNbO(3) by femtosecond laser micromachining, which is composed of optical waveguides inscripted by a femtosecond laser and embedded microelectrodes subsequently fabricated using femtosecond laser ablation and selective electroless plating. A half-wave voltage close to 19 V is achieved at a wavelength of 632.8 nm with an interaction length of 2.

Selective metallization on insulator surfaces with femtosecond laser pulses.

We report selective metallization on surfaces of insulators (glass slides and lithium niobate crystal) based on femtosecond laser modification combined with electroless plating. The process is mainly composed of four steps: (1) formation of silver nitrate thin films on the surfaces of glass or crystal substrates; (2) generation of silver particles in the irradiated area by femtosecond laser direct writing; (3) removal of unirradiated silver nitrate films; and (4) selective electroless plating in the modified area. We discuss the mechanism of selective metallization on the insulators.

Fabrication of microfluidic optical waveguides on glass chips with femtosecond laser pulses.

We describe the fabrication of microfluidic channel structures on the surface of a borosilicate glass slide by femtosecond laser direct writing for optical waveguide application. Liquid with a variable refractive index is fed into the microchannel, serving as the core of the waveguide. We demonstrate that either a multimode or a single-mode waveguide can be achieved by controlling the refractive index of the liquid.

[Probing R6G single molecules by surface-enhanced resonance Raman scattering].

R6G was incubated in three kinds of silver colloid with different low concentrations, and its surface-enhanced resonance Raman scattering (SERRS) was studied with a confocal microscopy system. Each sample exhibited different spectrum character. At the single-molecule level the SERRS-spectra were recorded in 10(-13) mol x L(-1) dye colloidal solution.