Accurate identification of 8-oxoguanine in RNA with single-nucleotide resolution using ligase-dependent qPCR.

8-oxoguanine (oG), a prevalent oxidative modification in RNA induced by reactive oxygen species (ROS), plays a pivotal role in regulating RNA functions. Accurate detection and quantification of oG modifications is critical to understanding their biological significance and potential as disease biomarkers, but effective detection methods remain limited. Here, we have developed a highly specific T3 DNA ligase-dependent qPCR assay that exploits the enzyme's ability to discriminate oG from guanine (G) with single-nucleotide resolution.

The Maternal Microbiome Programs the mA Epitranscriptome of the Mouse Fetal Brain and Intestine.

The microbiome exerts profound effects on fetal development and health, yet the mechanisms underlying remain elusive. N6-methyladenosine (mA) plays important roles in developmental regulation. Although it has been shown that the microbiome affects the mRNA mA modification of the host, it remains unclear whether the maternal microbiome affects mA epitranscriptome of the fetus so as to impact fetal development.

The Alteration of mA Modification at the Transcriptome-Wide Level in Human Villi During Spontaneous Abortion in the First Trimester.

A growing number of studies have demonstrated that N6 methyladenine (mA) acts as an important role in the pathogenesis of reproductive diseases. Therefore, it is essential to profile the genome-wide mA modifications such as in spontaneous abortion. In this study, due to the trace of human villi during early pregnancy, we performed high-throughput sequencing in villous tissues from spontaneous abortion (SA group) and controls with induced abortion (normal group) in the first trimester.

EEG-based vibrotactile evoked brain-computer interfaces system: A systematic review.

Recently, a novel electroencephalogram-based brain-computer interface (EVE-BCI) using the vibrotactile stimulus shows great potential for an alternative to other typical motor imagery and visual-based ones. (i) Objective: in this review, crucial aspects of EVE-BCI are extracted from the literature to summarize its key factors, investigate the synthetic evidence of feasibility, and generate recommendations for further studies. (ii) Method: five major databases were searched for relevant publications.

Facial Contour Rejuvenation by Reduction Malarplasty Combined With Second-Stage Fat Grafting.

Background: With aging, the facial contour shows a double-concave deformity. Conventional facial contouring procedure, including face lifting and fat grafting, cannot yield a smooth facial contour line. This study was the first to propose a combination of reduction malarplasty and 2nd-stage autologous fat grafting so as to achieve the aesthetic goals of facial contour rejuvenation.

[Spatial distribution and inter-annual variability of spawning grounds of Cynoglossus joyneri in the Yellow Sea coastal waters in summer].

With survey data from three voyages in the summer of 2014, 2016, and 2017, spatial distribution and inter-annual variability of spawning grounds of Cynoglossus joyneri in the coastal waters of Yellow Sea were examined based on Tweedie-generalized additive model (GAM). The results showed that the spawning grounds of C. joyneri mainly distributed in the Haizhou Bay ranging within 34°00'-35°18' N, 119°30'-121°30' E.

[Distribution and environmental characteristics of the spawning grounds of Scomberomorus niphonius in the coastal waters of Yellow Sea, China].

Japanese Spanish mackerel (Scomberomorus niphonius) is one of the main economic fish species in the Yellow Sea. To understand its spawning habitat and population dynamics in the early stage, we examined the distribution and environmental characteristics of spawning ground of Japanese Spanish mackerel using their egg survey data collecting in 2015 (late May) and 2017 (late May) in the coastal waters of Yellow Sea. The generalized additive model (GAM) with the Tweedie distribution was used to analyze the distribution and environmental characteristics of its spawning ground.

[Effects of environmental factors on catch distribution of stow net Pseudosciaena polyactis and Lophius litulon in southern Yellow Sea in spring].

Stow net fishery is one of the important fishing methods in southern Yellow Sea. Based on the 2006-2009 data from the stow net fishery resources survey in the southern Yellow Sea in spring, and by using the generalized linear model (GLM) and generalized additive model (GAM), this paper quantitatively analyzed the relationships between the catch distribution of Pseudosciaena polyactis and Lophius litulon and the environmental factors such as year, position, water depth, and sea surface temperature (SST). Compared with GLM model, GAM model could better explain the relationships between the spatiotemporal distribution of the catch per unit effort (CPUE) of P.