[Y chromosome microdeletion in azoospermia and severe oligozoospermia patients with or without varicocele:A comparative study].

Objective: To compare the incidences of Y chromosome microdeletion between patients with azoospermia or severe oligozoospermia with varicocele( VC) and those without VC and investigate the etiopathogenisis of their infertility.

Methods: We included 137 VC patients in group A(70 with azoospermia as group A1 and 67 with severe oligozoospermia as group A2),135 non-VC patients in group B(69 with azoospermia as group B1 and 66 with severe oligozoospermia as group B2),and 30 normal fertile men as controls in group C. We detected Y chromosome microdeletion in different groups using multiplex PCR.

[Correlation between di-2-ethylhexyl phthalate and idiopathic oligoasthenospermia].

Objective: To investigate the relationship between the level of di-2-ethylhexyl phthalate (DEHP) and idiopathic oligoasthenospermia by measuring the content of DEHP in the semen samples of different subjects.

Methods: We obtained semen samples from 100 infertile men with idiopathic oligoasthenospermia, 50 working all the year round in the plastic greenhouse (group A) and the other 50 constantly dining from plastic meal boxes (group B). We also enrolled 50 normal male volunteers as controls (group C).

[Screening of Y chromosome microdeletions in infertile males with varicocele].

Objective: To investigate the relationship between Y chromosome microdeletions and human spermatogenesis in infertile men with varicocele (VC).

Methods: We divided 174 infertile VC patients into groups A (with azoospermia, n = 47) , B (with severe oligozoospermia, n=57) and C (with mild oligozoospermia, n=70), and enlisted 28 fertile males and 26 fertile females as normal controls. We collected DNA from the peripheral blood, amplified 6 sequence tagged sites in AZFa, AZFb and AZFc using multiplex PCR technique.