[Histological Study on Integumentary and Digestive System of Hirudo nipponica].

Objective: The integumentary and digestive system of Hirudo nipponica were studied,in order to protect the precious Chinese herbal medicine.

Methods: The histological study of integumentary and digestive system of Hirudo nipponica by histological methods were carried out by microscopical method.

Results: The body wall of Hirudo nipponica could be divided into five layers, including cuticular layer, epithelial layer, dermis layer, muscle layer and grape-like tissue.

[Study on Reproductive Performance of Hirudio nipponica].

Objective: To investigate the reproductive performance of Hirudo nipponica under artificial cultivation, in order to provide reference for the commercial rearing and breeding industry of this species.

Methods: The mean number of cocoons, cocoon weight, hatching numbers of cocoons, and the effect of parent leeches weight and density on reproductive performance were investigated.

Results: The mean number of cocoons produced by parent leeches was 0.

Anti-herpes simplex virus activities of bioactive extracts from Antrodia camphorata mycelia.

Background: Antrodia camphorata, a traditional Chinese medicine, is widely used in the treatment of liver diseases and cancers. Anti-inflammatory properties have also been described. HSV infection represents one of the most serious public health concerns globally because of its devastating impact.

[Phenological characteristics of introduced Polygonum viviparum from high altitude to low altitude area].

Objective: To explore the phenological characteristics of Polygonum viviparum introduced from high altitude to low altitude area.

Methods: To introduce the root and seed of Polygonum viviparum from high altitude to low altitude area and collect their growth and phenological data.

Results: In low altitude area, Polygonum viviparum germinated at the beginning of March, grew from March to the end of July, withered during the whole August due to high temperature, recovered from the end of August to the beginning of October, and then withered again due to low temperature.

[Expression of subtilisin-like protease gene from Hirsutella sinensis before and after infecting Hepialus gonggaensis].

To address the role of Pr1 gene in the process of Hirsutella sinensis infecting Hepialus gonggaensis, differential expression of subtilisin-like protease gene was detected. In the present study, Pr1 gene analogues from H. sinensis were obtained by PCR strategy using specific primers designed from conserved regions of Pr1 gene reported in the GenBank.

[Study on toxic effects of ammonia on embryonic and yolk-sac stage larvae of rare minnow].

To investigate the toxicity to fish which were exposed to ammonia during early life stages. This study choose rare minnow which is unique to China for experimental subject. Representative toxicological endpoints were observed and recorded during the development of embryo and yolk-sac stage larvae.