Simultaneous detection and differentiation of classical Muscovy duck reovirus and goose-origin Muscovy duck reovirus by RT-qPCR assay with high-resolution melting analysis.

Introduction: Classical Muscovy duck reovirus (C-MDRV) and goose-origin Muscovy duck reovirus (Go-MDRV) infections cause "Liver white-spots disease" in Muscovy duckling and gosling. It is difficult to differentiate the infections caused by C-MDRV and Go-MDRV using conventional serological methods.

Methods: Specific primers were designed and synthesized according to σNS and λA nucleotide sequences of C-MDRV and Go-MDRV, respectively.

Ferroptosis, oxidative stress and hearing loss: Mechanistic insights and therapeutic opportunities.

Hearing loss, a prevalent sensory impairment, poses significant challenges worldwide. Recent research has shed light on the intricate interplay between ferroptosis, a newly recognized form of regulated cell death characterized by iron-dependent lipid peroxidation, and oxidative stress in the pathogenesis of hearing loss. In this review, we delve into the mechanisms underlying ferroptosis and oxidative stress in various forms of hearing loss, including age-related hearing loss (ARHL), noise-induced hearing loss (NIHL) ototoxic drug-induced hearing loss and genetic hearing loss.

Transient receptor potential channel 6 knockout ameliorates hepatic fibrosis by inhibiting the activation and proliferation of hepatic stellate cells.

Background And Aim: Hepatic fibrosis is a common outcome of chronic liver injury and can eventually lead to cirrhosis, which is a major public health concern. Hepatic stellate cells (HSCs) are the major producers of extracellular matrix (ECM) and regulate the synthesis and decomposition of ECM, but the specific mechanism of them remains unclear. Transient receptor potential channel 6 (TRPC6), a non-selective cation channel, plays an important role in organic fibrosis.

Implication of CCNG1 in radiosensitivity via the Wnt/β-catenin pathway in esophageal squamous cells.

Esophageal cancer (EC) poses a substantial threat to human health. The development of radioresistance in esophageal cancer cells is a critical factor contributing to local treatment failure and an unfavorable prognosis in affected patients. A comprehensive analysis was performed using bulk RNA sequencing (RNA-seq) and single-cell RNA sequencing (scRNA-seq) data from esophageal squamous cell carcinoma (ESCC) samples.

A porous network of boron-doped IrO nanoneedles with enhanced mass activity for acidic oxygen evolution reactions.

While proton exchange membrane water electrolyzers (PEMWEs) are essential for realizing practical hydrogen production, the trade-off among activity, stability, and cost of state-of-the-art iridium (Ir)-based oxygen evolution reaction (OER) electrocatalysts for PEMWE implementation is still prohibitively challenging. Ir minimization coupled with mass activity improvement of Ir-based catalysts is a promising strategy to address this challenge. Here, we present a discovery demonstrating that boron doping facilitates the one-dimensional (1D) anisotropic growth of IrO crystals, as supported by both experimental and theoretical evidence.

Prevention and Treatment of Alzheimer's Disease Via the Regulation of the Gut Microbiota With Traditional Chinese Medicine.

Alzheimer's disease (AD) is caused by a variety of factors, and one of the most important factors is gut microbiota dysbiosis. An imbalance in the gut mincrobiota have been shown to change the concentrations of lipopolysaccharide and short-chain fatty acids. These microorganisms synthesize substances that can influence the levels of a variety of metabolites and cause multiple diseases through the immune response, fatty acid metabolism, and amino acid metabolism pathways.

Tailoring structural and mechanical properties of konjac glucomannan/curdlan composite hydrogels by freeze-thaw treatment.

To improve the gelling properties of konjac glucomannan/curdlan (KGM/CUD) composite hydrogels, KGM/CUD composite hydrogels were treated by freeze-thawing. Herein, we focus on the effects of freeze-thaw cycles, freezing temperature, and freezing time on the structural and mechanical properties of KGM/CUD composite hydrogels. SEM and SAXS results showed that ice crystals generated by freezing extruded the molecular chains and increased the cross-linking density between molecular chains, which resulted in a denser gel microstructure.

[Experience of Integrative Palliative Care at Peking Union Medical College Hospital].

Objective To summarize the experience of integrative palliative care at Peking Union Medical College Hospital and provide a reference for promoting the integrative palliative care model. Methods Twenty cases receiving integrative palliative care at Peking Union Medical College Hospital were collected.The clinical characteristics,reasons for initiating integrative palliative care,the process of integrative palliative care,and feedback from these cases were summarized.

ΔNp63α promotes radioresistance in esophageal squamous cell carcinoma through the PLEC-KEAP1-NRF2 feedback loop.

Esophageal squamous cell carcinoma (ESCC) is one of the most aggressive cancers and is highly prevalent in China, exhibiting resistance to current treatments. ΔNP63α, the main isoform of p63, is frequently amplified in ESCC and contributes to therapeutic resistance, although the molecular mechanisms remain unknown. Here, we report that ΔNP63α is highly expressed in ESCC and is associated with radioresistance by reducing ROS level.

MOF-confined ultrafine nanozymes with enhanced catalysis for sensitive colorimetric detection of glucose.

The constrained enzymatic activity and aggregation challenges encountered of nanozymes pose an obstacle to their practical utility, necessitating a strategy to alleviate this problem and improve the enzymatic catalytic efficiency. Herein, MOF-confined ultrafine ZnIrO clusters (ZnIrO/ZnIrMOFs) nanozyme have been synthesized by controlling the in-situ growth of ZnIrO via a one-pot method. Notably, due to the domain-limiting effect of MOF, the ZnIrO clusters with small size grew uniformly in the structure of ZnIrMOFs nanosheets, while the presence of Zn atoms improved the crystallinity of the MOF phase and the dispersion of ZnIrO clusters.

nPOD-Kidney: A Heterogenous Donor Cohort for the Investigation of Diabetic Kidney Disease Pathogenesis and Progression.

Background: The Network for Pancreatic Organ donors with Diabetes-Kidney (nPOD-K) project was initiated to assess the feasibility of using kidneys from organ donors to enhance understanding of diabetic kidney disease (DKD) progression.

Methods: Traditional and digital pathology approaches were employed to characterize the nPOD-K cohort. Periodic acid-Schiff- and Hematoxylin and Eosin-stained sections were used to manually examine and score each nPOD-K case.

Biochanin A Attenuates Psoriasiform Inflammation by Regulating Nrf2/HO-1 Pathway Activation and Attenuating Inflammatory Signalling.

Psoriasis is a long-term inflammatory skin condition marked by an overabundance of keratinocytes and the release of pro-inflammatory cytokines in the outer layer of skin. For the comprehensive management of intermediate to advanced psoriasis, innovative biological treatments have been developed. Products for the superficial therapy of mild to moderate psoriasis are still necessary, though.

Pharmacological inhibition of KSper impairs flagellar pH homeostasis of human spermatozoa.

Background: Sperm-specific potassium channel (KSper) comprised of pore-forming subunit SLO3 and auxiliary subunit LRRC52 is of importance for sperm fertility. The deficiency of KSper in both mice and humans resulted in severe impairments of sperm functions including sperm hyperactivity and acrosome reaction. Previous reports suggested that mouse KSper modulated sperm function possibly by affecting sperm intracellular pH (pH).

Aqueous Electrocatalytic Hydrogenation Depolymerization of Lignin β-O-4 Linkage via Selective C-O(C) Bond Cleavage: The Regulation of Adsorption.

The cleavage of the benzene-oxygen (C-O(C)) bond of the lignin β-O-4 linkage is expected to relieve condensation of the degradation product and improve the product value. Nevertheless, the electrochemical breaking of the C-O(C) bond has not been achieved yet due to the high dissociation energy (∼409 kJ mol) and the easy over-reduction of aromatic compounds. Here, we report an aqueous electrochemical reduction strategy for breaking C-O(C) bonds via the regulation of molecular adsorption.

Psychological-Distress Factors in Patients With Breast Cancer: A Qualitative Meta-Synthesis.

Aims: To systematically review and integrate qualitative-research results pertaining to psychological distress in patients with breast cancer and to clarify its causes and drivers.

Background: Patients with breast cancer experience psychological distress in the stages of diagnosis, treatment, and rehabilitation, which seriously affects their quality of life. Through comprehensive qualitative research, the study comprehensively describes the experiences of patients with breast cancer to guide medical personnel in taking better care of them.

MSC-microvesicles protect cartilage from degradation in early rheumatoid arthritis via immunoregulation.

Objective: As research into preclinical rheumatoid arthritis (pre-RA) has advanced, a growing body of evidence suggests that abnormalities in RA-affected joint cartilage precede the onset of arthritis. Thus, early prevention and treatment strategies are imperative. In this study, we aimed to explore the protective effects of mesenchymal stem cell (MSC)-derived microvesicles (MVs) on cartilage degradation in a collagen-induced arthritis (CIA) mouse model.

Photoelectrochemical detection of Cu based on ZnInS/WO Z-scheme heterojunction.

A one-step hydrothermal technique was utilized to generate WO nanosheets on fluorine-doped tin oxide (FTO) (WO/FTO), which were subsequently modified with ZnInS microspheres to create a Z-scheme heterojunction ZnInS/WO/FTO electrode for Cu detection. The heterojunction exhibited excellent photoelectric conversion efficiency, which was nearly 2.5-fold and 5.