The effect of China's birth policy changes on birth defects-A large hospital-based cross-sectional study.

A retrospective analysis of birth data hospital-based obtained from 14 monitoring areas in the Huaihe River Basin from 2009 to 2019 was conducted. Trend in the total prevalence of birth defects (BDs) and subgroups were analyzed using the Joinpoint Regression model. The incidence of BDs increased gradually from 118.

DietaryAdvancedGlycationEnd Products-InducedCognitive Impairment in Aged ICR Mice: Protective Role of Quercetin.

Scope: Dietary advanced glycation products (dAGEs) have been reported to induce cognitive impairment while quercetin possesses potential neuroprotective effects. The aim is to explore whether dAGEs would induce similar cognitive impairment from both young and aged ICR mice, and the protective effects of quercetin.

Methods And Results: A total of 32 aged ICR mice (15-month-old) and 16 young ICR mice (3-month-old) are randomly assigned into the following six groups: Young mice control group, young mice fed with AGEs diet group, old mice control group, old mice fed with AGEs diet group, old mice with quercetin supplemented diet group, old mice fed with AGE diet supplemented with quercetin group.

Grape seed proanthocyanidin extract alleviates high-fat diet induced testicular toxicity in rats.

The present study aimed to investigate the protective effects of grape seed proanthocyanidin extract (GSPE) on high-fat diet (HFD) induced testicular damage, oxidative stress, and apoptotic germ cell death. Male rats ( = 40) were randomly divided into four groups: the control group (treated with physiological saline), HFD group, HFD + GSPE (100 mg kg) group and HFD + GSPE (300 mg kg) group. Compared with the HFD group the rats of the GSPE-treated group showed improved serum testosterone levels, sperm quality and histological appearance of the testis tissue.

Maternal Lead Exposure Induces Down-regulation of Hippocampal Insulin-degrading Enzyme and Nerve Growth Factor Expression in Mouse Pups.

Lead exposure is a known potential risk factor for neurodegenerative diseases such as Alzheimer's disease (AD). Exposure to lead during the critical phase of brain development has been linked with mental retardation and hypophrenia in later life. This study was aimed to investigate the effects of lead exposure of pregnant mice on the expressions of insulin-degrading enzyme (IDE) and nerve growth factor (NGF) in the hippocampus of their offspring.

The Effects of Early Life Lead Exposure on the Expression of Glycogen Synthase Kinase-3β and Insulin-like Growth Factor 1 Receptor in the Hippocampus of Mouse Pups.

The present study was undertaken to investigate the effects of maternal lead exposure on expression of GSK-3β and IGF1R in the hippocampus of mice offspring. Lead exposure initiated from beginning of gestation to weaning. Lead acetate administered in drinking solutions was dissolved in distilled deionized water at the concentrations of 0.

[Effects of genistein on proliferation and apoptosis in HT-29 cells].

Objective: The study is to explore the effects of genistein on proliferation and apoptosis in human colon cancer HT-29 cells and the likely underlying molecular mechanisms.

Methods: HT-29 cultures were maintained in DMEM containing 10% fetal bovine serum. Cell proliferation was determined by MTT assay and cell cycle distribution by cytometry.

[Retinoic acid induced cell cycle arrest and apoptosis in mouse embryonic palatal mesenchymal cells].

Objectives: To investigate the effect of all-trans retinoic acid (atRA) on proliferation activity and cell cycle distribution in mouse embryonic palatal mesenchymal (MEPM) cells and the underlying molecular mechanisms.

Methods: MEPM cells were prepared from palate shelves of mouse fetal on gestation day 13. Cell viability was determined by MTI assay.

[Effect of pueraria crude extreact and puerarin on ethanol-induced expression of heat shock protein 70 in embryonic mouse hippocampal cultures].

Objective: To study if the Pueraria crude extreact (CP) and standard preparation of pure puerarin (SP) possess the same neuroprotective effects on the expression of heat shock protein (HSP) 70 in the embryonic mouse hippocampal cells.

Methods: The hippocampus of 18-days-old mouse embryo was taken out and suspension of single cells was cultured. Ethanol was added to cause HSP70 mRNA expression.

[Effects of zearalenone on proliferation and apoptosis in MCF-7 cells].

Objective: To explore the effects of zearalenone (ZEA) on proliferation and apoptosis in estrogen-dependent human breast cancer MCF-7 cells and the likely underlying molecular mechanisms.

Methods: Cell viability was determined by MTT assay and cell cycle distribution by cytometry. Apoptosis was detected by Cell Death Detection ELISA and cytometry, respectively.

[Potential antiviral drug Pueraria crude extract and puerarin protect against ethanol-induced cytotoxicity in embryonic mouse hippocampal cultures].

Objective: To examine whether Chinese medical herb Pueraria crude extract (CP) and standard of pure puerarin (SP) possess the same neuroprotective effects during concomitant ethanol (EtOH) treatment.

Methods: Hippocampus cultures were prepared from mice at gestational age of 18 day. Cell viability was measured by MTT assay.

[Effects of environmental estrogens on apoptosis induced by estrogen depletion in T47D cells].

Objective: To explore the effects of environmental estrogens (n-4-noniphenol, NP; bisphenol, BisA; and dibutylphthalate, DBP) on apoptosis induced by estrogen depletion in breast cancer T47D cells.

Methods: Human T47D breast cancer cells were grown in DMEM medium containing 10% bovine serum. Four days before adding the test compounds, the cells were washed in phosphate-buffered saline, and the medium was substituted with a phenol red-free DMEM medium containing 5% dextral charcoal-stripped FBS.

[Effects of genistein and zearalenone on proliferation of PEO4].

Objective: The objective of this study was to investigate the estrogenic activity of genistein and zearalenone through their effects on the proliferative capacity of human ovarian PEO4.

Methods: Estrogen receptor-positive PEO4 cell was grown in DMEM medium containing 10% bovine serum. Five days before the addition of the test compounds, the cells were washed in phosphate-buffered saline, and the medium was substituted with a phenol red-free DMEM medium containing 5% dextran charcoal-stripped FBS.

[The effects of three plastic additives on the proliferation of MCF-7 cell].

Objective: To explore the effect of environmental estrogens on the proliferation of breast cancer cell line MCF-7.

Methods: The tested compounds were n-4-nonyphenol, Bisphenol A and dibutylphthalate. Human estradiol-dependent MCF-7 breast cancer cells were grown in DMEM medium containing 10% bovine serum.

Effect of zinc on bone metabolism in fetal mouse limb culture.

Objective: To determine the effects of zinc-deficiency and zinc-excess on bone metabolism.

Methods: We developed the culture model of fetal mouse limbs (16th day) cultivated in self-made rotator with continuing flow of mixed gas for six days in vitro. The cultured limbs were examined by the techniques of 45Ca tracer and X-roentgenography.