Peptide-Driven Proton Sponge Nano-Assembly for Imaging and Triggering Lysosome-Regulated Immunogenic Cancer Cell Death.

Triggering lysosome-regulated immunogenic cell death (ICD, e.g., pyroptosis and necroptosis) with nanomedicines is an emerging approach for turning an "immune-cold" tumor "hot"-a key challenge faced by cancer immunotherapies.

A novel severe cerebral venous thrombosis rat model based on semi-ligation combined with ferric chloride and thrombin.

Aims: An applicable cerebral venous sinus thrombosis (CVST) model is imperative for exploring its pathophysiology. We established a novel severe CVST model using semi-ligation, ferric chloride, and thrombin.

Methods: A total of 138 male Sprague-Dawley rats were randomly divided into semi-ligation (n = 75) and non-semi-ligation (n = 63) groups.

Glioma Imaging by O-(2-18F-Fluoroethyl)-L-Tyrosine PET and Diffusion-Weighted MRI and Correlation With Molecular Phenotypes, Validated by PET/MR-Guided Biopsies.

Gliomas exhibit high intra-tumoral histological and molecular heterogeneity. Introducing stereotactic biopsy, we achieved a superior molecular analysis of glioma using O-(2-18F-fluoroethyl)-L-tyrosine (FET)-positron emission tomography (PET) and diffusion-weighted magnetic resonance imaging (DWI). Patients underwent simultaneous DWI and FET-PET scans.

RELA Fusion in Supratentorial Extraventricular Ependymomas: A Morphologic, Immunohistochemical, and Molecular Study of 43 Cases.

Supratentorial extraventricular ependymomas (STEEs) are relatively rare ependymomas, and their pathologic and genetic characteristics are still poorly understood. The aim of this study was to determine the histologic, immunohistochemical, and RELA fusion features, as well as to clarify in more detail the clinical courses of STEEs. Data from a total of 43 patients with STEEs was analyzed retrospectively.

H3 K27M-mutant diffuse midline gliomas in different anatomical locations.

The histone H3 K27M mutation has been frequently reported in most diffuse midline gliomas. However, the relationship between the H3 K27M mutation and clinical outcomes of gliomas from different anatomical locations is still not fully understood. A total of 120 patients with diffuse midline gliomas were selected for this retrospective observational study.

NHC Pd(II) and Ag(I) Complexes: Synthesis, Structure, and Catalytic Activity in Three Types of C-C Coupling Reactions.

Four bis-benzimidazolium salts, 1,4-bis[1'-(-R-benzimidazoliumyl)methyl]-2,3,5,6-tetramethylbenzene 2X ( : R = ethyl, X = PF; : R = picolyl, X = Br; : R = benzyl, X = Br; and : R = allyl, X = Br), and their three -heterocyclic carbene (NHC) Pd(II) and Ag(I) complexes, (), (), and (), as well as one anionic complex (), have been synthesized and characterized. Complex adopts a funnel-like type of structure, complex adopts a cyclic structure, and complex is an open structure. In the crystal packing of -, one-dimensional polymeric chains and two-dimensional supramolecular layers are formed via intermolecular weak interactions, including hydrogen bonds, π-π interactions, and C-H···π contacts.

Comparative assessment of three methods to analyze MGMT methylation status in a series of 350 gliomas and gangliogliomas.

MGMT promoter methylation is considered as a prognostic and predictive biomarker indicating response to chemotherapy and radiotherapy in glioblastoma. A number of different methods and platforms including pyrosequencing (PSQ), quantitative methylation-specific PCR (qMSP) and immunohistochemistry (IHC), methylation-sensitive high resolution melting (MS-HRM) and NGS (Next Generation Sequencing) have been used to detect MGMT promoter methylation in gliomas. However, controversy remains about the most appropriate method to use for analyzing MGMT status.

Synthesis and structural studies of N-heterocyclic carbene Ag(I) and Hg(II) complexes and recognition of dihydrogen phosphate anion.

Bis-benzimidazolium salt (S)-2,2'-bis[2″-(N-Et-benzimidazoliumyl)ethoxy]-1,1'-binaphthyl hexafluorophosphate [(S)-LH]·(PF) and bis-imidazolium salts (S)-2,2'-bis[2″-(N-R-imidazoliumyl)ethoxy]-1,1'-binaphthyl hexafluorophosphate [(S)-LH]·(PF) and [(S)-LH]·(PF) (R = ethyl or benzyl), as well as their five N-heterocyclic carbene Hg(II) and Ag(I) complexes such as [(S)-LHg(HgBr)] (1), [(S)-LHg(HgBr)] (2), [(S)-LHg(HgI)] (3), {[(S)-LAg](PF)} (4) and [(S)-LAg](PF) (5) have been prepared and characterized. Each of complexes 1-3 consists of two rings (one 6-membered ring and one 11-membered ring), in which the oxygen atom in the ligand participates in coordination with Hg(II) ion. In complex 4, 1D helical polymeric chain is formed via biscarbene ligand (S)-L and Ag(I) ion.

[Correlation of chromosome 1p and 19q status and expression of R132H mutant IDH1 protein in oligodendroglial tumors].

Objective: To correlate the presence of chromosome 1p/19q deletion with the expression of R132H mutant IDH1 status in oligodendroglial tumors, and to explore molecular markers for predicting chemosensitivity of oligodendroglial tumors.

Methods: The study included 75 oligodendroglial tumors (38 oligodendrogliomas and 37 oligoastrocytomas). Immunohistochemistry was used to detect the expression of R132H mutant IDH1 protein, and fluorescence in situ hybridization (FISH) was employed to detect 1p/19q deletion.