Background: Automated hematology analyzer is widely used by clinical laboratories to examine blood cell counts and differential leukocyte counts. However, a microscopic examination of a well-prepared blood film is still necessary and useful in most cases.
Methods: Two patients with severe disease were required complete blood counts (CBC) after admission at West China Second University Hospital in Southwest China.
Objective: To analyse the difference of genome-wide DNA methylation status of CpG island between the monozygotic twins with disconcordant phenotype of HBV infection and to discover possible differentially methylated genes.
Methods: Modified AIMS (amplification of inter-methylated sites) method was adopted. According to the frequent sites of CpG islands (-CGCG- and -CCGG-), three groups of isochizomers with distinct methylation sensitivity (Sma I-Xma I, Hpa II-Msp I and BssH II-Pau I/BseP I) were used to modify the AIMS method.
Sichuan Da Xue Xue Bao Yi Xue Ban
July 2004
Objective: To discuss the influence of abnormally higher white blood cell (WBC) on hemoglobin (Hb) determination and work for its redress.
Methods: High WBC concentration suspensions of 48 concentration gradients were prepared to be determined in the pre-dilution pattern of automated hematology analyzer while the changes of Hb concentration before and after removal of WBC being observed. At the same time, a regression equation was set up to correct errors and redress the Hb concentration.
Hua Xi Yi Ke Da Xue Xue Bao
January 2002
Objective: To investigate the effects of CCK8 on protein kinase C activity in rat cerebral cortex.
Methods: The cerebral cortex neurocytes were isolated and used as a model. The effects of CCK8, L-364, 718 and L-365, 260 on PKC activities were detected by using a non-radioactive method.