Continuous cropping of Patchouli alters soil physiochemical properties and rhizosphere microecology revealed by metagenomic sequencing.

Continuous cropping (CC) profoundly impacts soil ecosystems, including changes in soil factors and the structure and stability of microbial communities. These factors are interrelated and together affect soil health and plant growth. In this research, metagenomic sequencing was used to explore the effects of CC on physicochemical properties, enzyme activities, microbial community composition, and functional genes of the rhizosphere soil of patchouli.

Rubber intercropping with arboreal and herbaceous species alleviated the global warming potential through the reduction of soil greenhouse gas emissions.

Agroforestry systems are known to enhance soil health and climate resilience, but their impact on greenhouse gas (GHG) emissions in rubber-based agroforestry systems across diverse configurations is not fully understood. Here, six representative rubber-based agroforestry systems (encompassing rubber trees intercropped with arboreal, shrub, and herbaceous species) were selected based on a preliminary investigation, including Hevea brasiliensis intercropping with Alpinia oxyphylla (AOM), Alpinia katsumadai (AKH), Coffea arabica (CAA), Theobroma cacao (TCA), Cinnamomum cassia (CCA), and Pandanus amaryllifolius (PAR), and a rubber monoculture as control (RM). Soil physicochemical properties, enzyme activities, and GHG emission characteristics were determined at 0-20 cm soil depth.

Identification and Molecular Characterization of Telosma Mosaic Virus (TelMV) and East Asian Passiflora Virus (EAPV) from Patchouli in China.

Patchouli is a valuable medicinal herb and cash crop in China, but viral infections cause significant yield losses. This study identified six viruses in patchouli transcriptome data, including the first-ever detection of East Asian Passiflora Virus (EAPV) in patchouli. RT-PCR validated three viruses from diseased patchouli plants in Haikou, China: telosma tosaic virus (TelMV), broad bean wilt virus-2 (BBWV-2), and pogostemom alphacytorhabdovirus 1 (PogACRV1_Pog).

Continuous Cropping of Patchouli Alleviate Soil Properties, Enzyme Activities, and Bacterial Community Structures.

(Patchouli), an essential medicinal plant in the family, faces significant challenges under continuous cropping (CC) obstacles. This study examined the rhizospheric soil bacterial communities of patchouli under four different CC years, zero (CK), one (T1), two (T2), and three (T3) years through high-throughput 16S rRNA gene amplicon sequencing. Results showed long-term CC led to significant soil properties and enzyme activity shifts.

Glutathione and Ascorbic Acid Accumulation in Mango Pulp Under Enhanced UV-B Based on Transcriptome.

Mango (), a nutritionally rich tropical fruit, is significantly impacted by UV-B radiation, which induces oxidative stress and disrupts physiological processes. This study aimed to investigate mango pulp's molecular and biochemical responses to UV-B stress (96 kJ/mol) from the unripe to mature stages over three consecutive years, with samples collected at 10-day intervals. UV-B stress affected both non-enzymatic parameters, such as maturity index, reactive oxygen species (ROS) levels, membrane permeability, and key enzymatic components of the ascorbate-glutathione (AsA-GSH) cycle.

Transcriptomic Insights into Salt Stress Response in Two Pepper Species: The Role of MAPK and Plant Hormone Signaling Pathways.

Salt stress imposes significant plant limitations, altering their molecular, physiological, and biochemical functions. Pepper, a valuable herbaceous plant species of the family, is particularly susceptible to salt stress. This study aimed to elucidate the physiological and molecular mechanisms that contribute to the development of salt tolerance in two pepper species ( (moderate salt tolerant) and (salt sensitive)) through a transcriptome and weighted gene co-expression network analysis (WGCNA) approach to provide detailed insights.

Genome-Wide Identification and Expression Analysis of the Casparian Strip Membrane Domain Protein-like Gene Family in Peanut ( L.) Revealed Its Crucial Role in Growth and Multiple Stress Tolerance.

Casparian strip membrane domain proteins (CASPs), regulating the formation of Casparian strips in plants, serve crucial functions in facilitating plant growth, development, and resilience to abiotic stress. However, little research has focused on the characteristics and functions of in cultivated peanuts. In this study, the genome-wide identification and expression analysis of the gene family was performed using bioinformatics and transcriptome data.

Identification of the gene family in patchouli and functional analysis in response to continuous cropping stress.

To further reveal the molecular mechanisms underlying the formation of continuous cropping disorders in patchouli, this study analyzed the function of calcium dependent protein kinase () genes at the molecular level in patchouli continuous cropping disorders. The findings unveiled the presence of 45 genes within the complete patchouli genome dataset. These genes exhibited a range of molecular weights from 50.

A Genome-Wide Identification and Expression Analysis of the Casparian Strip Membrane Domain Protein-like Gene Family in in Response to p-HBA-Induced Continuous Cropping Obstacles.

Casparian strip membrane domain protein-like () genes are key genes for the formation and regulation of the Casparian strip and play an important role in plant abiotic stress. However, little research has focused on the members, characteristics, and biological functions of the patchouli gene family. In this study, 156 genes were identified at the whole-genome level.

cDNA cloning, prokaryotic expression and functional analysis of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) in Pogostemon cablin.

Pogostemon cablin is an important commercial source of patchouli oil, whose main active ingredient is patchouli alcohol. This sesquiterpene is a product of the mevalonate pathway, in which 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase (HMGCR) is the rate-limiting enzyme. In this study, P.