Roots applicable, high sensitivity and specificity assay for the detection of Liberibacter asiaticus in roots and fruits.

Liberibacter asiaticus (CLas), a phloem-limited Gram-negative bacterium, is associated with citrus huanglongbing (HLB), which is one of the most destructive diseases currently threatening citrus production worldwide. No effective treatment for HLB is currently available. Effective prevention and control in the initial stage can block the spread and disease progression of HLB.

Sequence-specific nanoparticle barcode strategy for multiplex human enterovirus typing.

Human enteroviruses (HEV) can cause a range of diseases from mild to potentially life-threatening. Identification and genotyping of HEV are crucial for disease management. Existing typing methods, however, have inherent limitations.

An Inhibitor-Monitorable Single-Tube Duplex Quantitative Real-Time PCR Assay for the Detection of ' Liberibacter asiaticus'.

Huanglongbing (HLB) is a citrus infectious disease caused by ' Liberibacter' spp. Recently, it has begun to spread rapidly worldwide, causing significant losses to the citrus industry. Early diagnosis of HLB relies on quantitative real-time PCR assays.

An encodable multiplex microsphere-phase amplification sensing platform detects SARS-CoV-2 mutations.

SARS-CoV-2 variants of concern (VOCs) contain several single-nucleotide variants (SNVs) at key sites in the receptor-binding region (RBD) that enhance infectivity and transmission, as well as cause immune escape, resulting in an aggravation of the coronavirus disease 2019 (COVID-19) pandemic. Emerging VOCs have sparked the need for a diagnostic method capable of simultaneously monitoring these SNVs. To date, no highly sensitive, efficient clinical tool exists to monitor SNVs simultaneously.

Accurate nucleic acid quantification in a single sample tube without the need for calibration.

Convenient and accurate nucleic acid quantification (NAQ) is crucial to clinical diagnosis, forensic medicine, veterinary medicine and food analysis. However, traditional NAQ relies on the preparation of a laborious, time-consuming and expensive calibration curve, which would also propagate pipette errors through serially dilutions. Besides, traditional NAQ is run in different tubes, which introduces bias from random tube-to-tube variations and is unable to detect inhibitors from biological samples.

Room-temperature-storable PCR mixes for SARS-CoV-2 detection.

Objectives: A novel coronavirus (severe acute respiratory syndrome coronavirus 2, SARS-CoV-2) emerged in late 2019, causing an outbreak of pneumonia [coronavirus disease 2019 (COVID-19)] globally. Although the use of ready-made reaction mixes can enable more rapid PCR-based diagnosis of COVID-19, the need to transport and store these mixes at low temperatures presents challenges to already overburdened logistics networks.

Methods: Here, we present an optimized freeze-drying procedure that allows SARS-CoV-2 PCR mixes to be transported and stored at ambient temperatures, without loss of activity.

[Advances in multiple PCR technology studies].

Multiple PCR (Multiplex polymerase chain reaction, MPCR) is a technology to simultaneously amplify multiple targets through a single reaction and to detect the amplification products by reliable detection means so as to realize the diagnosis of multiple targets. MPCR has been well studied for its high efficiency, high throughput and low cost. At present, MPCR has been widely used in scientific research, disease diagnosis and other fields.