[Establishment and identification of recombinant NS-1 cell strain that stably expresses chimeric HBc containing HBV multiepitope short peptides].

Objective: To establish recombinant NS-1 cell strain that is capable of stable expression of chimeric HBc particle containing HBV multi epitope short peptides.

Methods: The recombinant plasmid, pHBc-Mep, was transfected into NS-1 cells via Lipofectamine, and the recombinant cell strain was screened with G418 and subclone screening. The expression products of the cells were examined by RT-PCR, ELISA, indirect immunofluorescence assay (IFA) and Western blotting.

[Cloning and expression of hepatitis C core protein gene].

Objective: To express hepatitis C virus (HCV) core protein gene fragment in E. coli.

Methods: A fragment of HCV core gene sequence 357 bp in length was amplified by PCR, digested with EcoRI+Hind III and inserted to the plasmid vector pET-32a to construct recombinant HCVc/pET-32a plasmid, which was transformed into E.

Expression and immunocompetence identification of Plasmodium falciparum glutamate dehydrogenase.

Objective: To express the fusion protein of glutamate dehydrogenase (GDH) with glutathione S-transferase (GST) of Plasmodium falciparum FCC1/HN in E. coli BL21 and assess the immunocompetence of the recombinant protein.

Methods: GDH gene of P.