Copper-Catalyzed Difluoroalkylation Reaction.

This review describes recent advances in copper-catalyzed difluoroalkylation reactions. The RCF radical is generally proposed in the mechanism of these reactions. At present, various types of copper-catalyzed difluoroalkylation reactions have been realized.

Dual-function monolithic enzyme reactor based on dopamine/graphene oxide coating for simultaneous protein enzymatic hydrolysis and glycopeptide enrichment.

A new dual-function enzyme reactor was prepared based on a dopamine/graphene oxide coated boron affinity monolithic column, which can be used for simultaneous protein enzymatic hydrolysis and glycopeptide enrichment. Firstly, a boron affinity monolithic column was prepared as the carrier for enzyme reactor. Secondly, the monolithic column was coated with dopamine/graphene oxide to provide higher specific surface area for the increase in the amount of trypsin bound.

Improving the Identification of Lysine-Acetylated Peptides Using a Molecularly Imprinted Monolith Prepared by a Deep Eutectic Solvent Monomer.

To overcome the identification challenge of low-abundance lysine acetylation (Kac), a novel approach based on a molecularly imprinted polymer (MIP) was developed to improve the extraction capacity of Kac peptides in real samples. Green deep eutectic solvents (DESs) were introduced and used as one of the synergistic functional monomers with zinc acrylate (ZnA). Glycine-glycine-alanine-lysine(ac)-arginine (GGAKacR) was chosen as a template and ,'-methylenbisacrylamide (MBAA) was used as a cross-linker.

Construction of a microfluidic platform integrating online protein fractionation, denaturation, digestion, and peptide enrichment.

Microfluidic system with multi-functional integration of high-throughput protein/peptide separation ability has great potential for improving the identification capacity of biological samples in proteomics. In this paper, a sample treatment platform was constructed by integrating reversed phase chromatography, immobilized enzyme reactor (IMER) and imprinted monolith through a microfluidic chip to achieve the online proteins fractionation, denaturation, digestion and peptides enrichment. We firstly synthesized a poly-allyl phenoxyacetate (AP) monolith and a lysine-glycine-glycine (KGG) imprinted monolith separately, and investigated in detail their performance in fractionating proteins and extracting KGG from the protein digests of MCF-7 cell.

Preparation, characterization, and application of soluble liquid crystalline molecularly imprinted polymer in electrochemical sensor.

A novel soluble molecularly imprinted polymer (SMIP) without chemical cross-linker was successfully synthesized. The quinine (QN), which the structure was similar to the template, was chosen as the immobile template to improve the affinity of MIP. 4-Methyl phenyl dicyclohexyl ethylene (MPDE) was used as the liquid crystal (LC) monomer to increase the rigid of the composite.

Improving affinity of imprinted monolithic polymer prepared in deep eutectic solvent by metallic pivot.

One of the major drawbacks of conventional molecularly imprinted polymers (MIPs) is the requirements of volatility porogenic solvent during polymerization. To overcome the default, MIP based on deep eutectic solvent (DES, a new type of green designer solvents) has been synthesized successfully. To improve the affinity of the MIP based on DES, in this work, a strategy of metallic pivot was suggested in the first time to prepare a highly selective MIP monolithic column.

[Research progress in developing reporter systems for the enrichment of positive cells with targeted genome modification].

Targeted genome editing technology plays an important role in studies of gene function, gene therapy and transgenic breeding. Moreover, the efficiency of targeted genome editing is increased dramatically with the application of recently developed artificial nucleases such as ZFNs, TALENs and CRISPR/Cas9. However, obtaining positive cells with targeted genome modification is restricted to some extent by nucleases expression plasmid transfection efficiency, nucleases expression and activity, and repair efficiency after genome editing.

Molecularly imprinted nanoparticles with nontailing peaks in capillary electrochromatography.

The combination of microparticles of molecularly imprinted polymers (MIPs) with partial filling capillary electrochromatography (CEC) has previously been demonstrated for the enantiomer separation. In this paper, precipitation polymerization was used to prepare d-zopiclone imprinted nanoparticles (50-80 nm) by a strategy of the dilution of pre-polymerization mixtures. The influence of some important parameters on the preparation of MIPs nanoparticles, including template to monomer ratio, type and amount of cross-linking monomer, and functional monomer composition ratio were investigated.

Preparation and characterization of grafted imprinted monolith for capillary electrochromatography.

In this paper, a molecularly imprinted polymer (MIP) coating grafted to a trimethylolpropane trimethacrylate (TRIM) core material for CEC was reported. The core monolith was prepared with a solution of 20% (w/w) TRIM in a mixture of porogen and a polymerization precursor, which can generate a stable electroosmotic flow due to the formation of ionizable groups after postpolymerization hydrolization. Graft polymerization took place on the resultant TRIM monolith with a mixture of template, methacrylic acid, and ethylene glycol dimethacrylate.

Low crosslinking imprinted coatings based on liquid crystal for capillary electrochromatography.

Low loading capacity is the main problem of molecularly imprinted stationary phase, which is attributed to the high level of crosslinking restricting distortion phenomena of polymer backbone in molecular imprinting. A new approach based on liquid crystal with recognition ability is demonstrated for synthesis of molecularly imprinted polymer coatings in a low level of crosslinking. The resulting low crosslinking (20%) open-tubular imprinted capillary was able to separate enantiomers by means of capillary electrochromatography.

Coatings of one monomer molecularly imprinted polymers for open tubular capillary electrochromatography.

One monomer molecularly imprinted polymer coatings were first synthesized in fused silica capillary columns with 2-methacrylamidopropyl methacrylate (MAM) as single functional monomer in addition to a cross-linking monomer. Since MAM may generate no or little EOF, a strategy of precursor of polymerization, which does not interfere with the formation of defined imprints, was used to introduce an ionizable functional monomer to generate a stable electroosmotic flow for electrochromatography (CEC) by post-polymerization hydrolization. The resulting MAM-based open-tubular imprinted capillary was able to separate enantiomers by means of CEC.