Predation of Cyclopoid Copepods on the Theronts of : Shedding Light on Biocontrol of White Spot Disease.

White spot disease, caused by the parasitic ciliate , is a significant threat to the freshwater fish farming industry worldwide, resulting in massive mortality and economic losses. Eliminating the free-swimming theronts from the culture environment is considered crucial for the control of infection. It is well-documented that planktonic ciliates are valuable food resources for macro-zooplankton in aquatic ecosystems.

Characterization of the complete mitochondrial genome of Yamaguti and Miyata, 1940 (Cestoda: Nippotaeniidae).

In this study, we report the first complete mitochondrial genome of the tapeworm in the order Nippotaeniidea Yamaguti, 1939. This mitogenome, which is 14,307 base pairs (bp) long with an A + T content of 72.2%, consists of 12 protein-coding genes, 22 transfer RNA (tRNA) genes, two rRNA genes, and two non-coding regions.

[Expression of peroxiredoxin III in cervical lesions].

Objective: To investigate the expression feature of peroxiredoxin III in cervical lesions and to further understand the mechanism for cervical cancer development/progression.

Methods: Expression of peroxiredoxin III was immunohistochemically detected in cervical cancer. In addition, cervical epithelia were transfected with recombinant adeno-associated virus vector containing human papillomavirus 16 E6/E7 and peroxiredoxin III expression was detected by quantitative real time PCR and Western blotting.

[The effects of progesterone and insulin-like growth factor (IGF- I and IGF-II) on proliferation in human decidual stromal cells of early pregnancy in vitro].

Objective: To study the effect of progesterone and Insulin-like growth factor (IGF-I and IGF-II) on proliferation in human decidual stromal cells of early pregnancy in vitro.

Methods: [3H]Thymidine (3H-TdR) uptake was applied to assess cell proliferation in human decidual stromal cells of early pregnancy (gestation of 5 to 7 weeks) in vitro after cultured with progesterone, IGF-I or IGF-II.

Results: Progesterone, IGF-I and IGF-II stimulated cell proliferation by 1.

[The affection of antigen presentation ability, which sources from the spleen macrophage of Balb/c mouse during late gestation, on maternal Th2/Th1 type of immune].

Objective: To study the relationship between antigen presentation ability of spleen macrophage and maternal Th2>Th1 immune bias in Balb/c mice during late pregnancy.

Methods: Balb/c mice during late gestation were adopted in our study, and mice of same species in estrus were used as control. With antigen stimulation, the spleen macrophages of Balb/c mice were pulsed as antigen presentation cells (APC).

[P53 codon 72 polymorphism in cervical cancers and its correlation with HPV16,18E6].

Objectives: To examine p53 codon 72 polymorphism in cervical cancers and its correlation with HPV16/18 E6.

Methods: Cervical specimens were taken from 81 patients with cervical squamous cancer, 18 patients with cervical adenocarcinoma, 88 patients with CIN II - III and 60 patients without cancers. PCR was used to examine the p53 genotypes and the expression of HPV16 and 18 E6.

[Evaluation of the effect of fertility-saving surgery on young patients with malignant ovarian tumors].

Objective: To evaluate the impact of fertility-saving surgery and adjuvant chemotherapy on survival and fertility of young patients with ovarian malignant tumors.

Methods: A retrospective analysis was done on 38 patients with ovarian malignant germ cell tumors, 22 patients with malignant epithelial tumors and 4 patients with sexual cord mesenchymal tumors receiving conservative treatments. Outcomes such as menstruation and reproduction ability were assessed.

[Comparison of therapeutical effect between arterial interventional chemotherapy and venous chemotherapy on bulky or locally advanced cervical cancer].

Objective: To investigate the therapeutical effect of arterial interventional chemotherapy and venous chemotherapy on bulky or locally advanced cervical cancer.

Methods: A retrospective study was carried out on 174 patients with bulky or locally advanced cervical cancers admitted to our hospital from Sept 1997 to Sept 2003. The therapeutical, toxic and adverse effects of either arterial interventional or venous chemotherapy were analyzed and compared.

[Study of thalidomide on the growth and angiogenesis of ovary cancer SKOV3 transplanted subcutaneously in nude mice].

Objective: To study the effect of thalidomide (Thd) used alone and in combination with cytoxan (CTX) on the growth and angiogenesis of human ovarian cancer transplanted subcutaneously in nude mice.

Methods: Human ovarian cancer model transplanted subcutaneously in nude mice was established, and divided into 3 groups: control group, Thd group, and Thd + CTX group. Tumor volume and weight, vascular endothelial growth factor (VEGF) mRNA, VEGF protein, microvascular density (MVD) were detected.

[Immortalization of human embryonic cervical epithelial cells induced by E6, E7 genes of human papillomavirus 16].

Objective: To establish an immortalized cell line derived from the embryonic cervical epithelium by infection with the recombinant adeno-associated virus (rAAV) containing human papillomavirus (HPV)16 E6, E7, and to study the biological features of cervical cancer cell line.

Methods: Human embryonic cervical tissues were cultured in keratinocyte free serum (K-FS) medium and infected with rAAV containing HPV16 E6, E7. Morphological features and growth rate were examined by light, electronic and fluorescence microscopies.

[Induction of human oral carcinoma by human papillomavirus 16 E6/E7 and TPA].

Background: To study the effect of human papillomavirus (HPV) 16 E6/E7 and TPA (12-O-tetradecanog-1-phorbol-13-acetate) on malignant transformation of human embryo oral tissue.

Methods: Recombinant plasmid with HPV 16 E6/E7 was constructed and transfected into human embryo oral tissue. The oral tissue with HPV 16 E6/E7 gene or without the gene was inoculated into the hypophloeodal of right shoulder in scid mice, respectively.

[Study on the TIL and NK of IL-2 injected via pelvic retroperitoneal space in gynecological cancer patient].

Objective: To verify the feasibility and effect of biotherapy instituted via pelvic retroperitoneal space on gynecological cancer.

Methods: Injecting IL-2 (and/or) 5-Fu through a tube installed in the pelvic retroperitoneal space. Counting the subpopulation of T cell and NK of lymph-nodes of pelvis after the drugs being by FCM.