Antibody-mediated clearance of an ER-resident aggregate that causes glaucoma.

Recombinant antibodies are a promising class of therapeutics to treat protein misfolding associated with neurodegenerative diseases, and several antibodies that inhibit aggregation are approved or in clinical trials to treat Alzheimer's disease. Here, we developed antibodies targeting the aggregation-prone β-propeller olfactomedin (OLF) domain of myocilin, variants of which comprise the strongest genetic link to glaucoma and cause early onset vision loss for several million individuals worldwide. Mutant myocilin aggregates intracellularly in the endoplasmic reticulum (ER).

Root-associated microbial diversity and metabolomics in maize resistance to stalk rot.

As one of the three major food crops in the world, maize plays a significant role in alleviating the food crisis. Maize stalk rot can reduce maize yield and mechanical harvesting efficiency. In addition, mycotoxins such as Deoxynivalenol (DON) and Zearalenone (ZEN) produced by maize stalk rot pathogens can also harm livestock and human health.

The effects of different doses of compound enzyme preparations on the production performance, meat quality and rumen microorganisms of yak were studied by metagenomics and transcriptomics.

Yak () is a large ruminant endemic to the Tibetan plateau. The addition of enzyme complexes to feed can significantly improve their growth performance. Therefore, studying the effects of ruminant compound enzyme preparations dosage on yak rumen microorganisms and production performance is crucial to promoting the development of the yak industry.

The causal relationship between 233 metabolites and coronary atherosclerosis: a Mendelian randomization study.

Objective: To investigate the causal relationship between 233 newly reported metabolites and coronary atherosclerosis through Mendelian randomization analysis.

Methods: Five different methods were used to perform Mendelian randomization analysis on the 233 metabolites and coronary atherosclerosis, with inverse variance weighting as the primary result, supplemented by other methods.

Results: The analysis identified that certain metabolites increase the susceptibility risk of coronary atherosclerosis, including: Total fatty acids (OR = 1.

Changes in sST2 and NT-proBNP levels predict early cardiac arrhythmia in breast cancer patients treated with anthracycline-containing chemotherapies.

Background: Cardiovascular biomarkers are crucial for monitoring cancer therapy-related cardiac toxicity, but the effects on early stage are still inadequate. To screen biomarkers in patients with breast cancer who receive anthracycline-containing chemotherapy, we studied the behavior of six biomarkers during chemotherapy and their association with chemotherapy-related cardiac toxicity.

Methods: In a prospective cohort of 73 patients treated with anthracycline-containing chemotherapy, soluble suppression of tumorigenicity 2 (sST2), high-sensitivity cardiac troponin T, N-terminal pro-B-type natriuretic peptide (NT-proBNP), myoglobin, creatine kinase isoenzyme MB, and heart-fatty acid binding protein were measured at baseline, during chemotherapy cycle (C1-C6).

Causal Associations Between Sleep Traits and Delirium: A Bidirectional Two-Sample Mendelian Randomization Study.

Purpose: Numerous studies have identified a correlation between sleep and delirium; however, the causal relationship remains ambiguous. This bidirectional two-sample Mendelian randomization (MR) study was conducted to examine the possible causal relationships between sleep traits and delirium.

Patients And Methods: Utilizing genome-wide association studies (GWAS), we identified ten sleep traits: chronotype, sleep duration, short sleep duration, long sleep duration, daytime napping, daytime sleepiness, insomnia, number of sleep episodes (NSE), sleep efficiency, and rapid eye movement sleep behavior disorder (RBD).

Bronchoscopic Closure of Bronchopleural Fistula with Occluder.

Bronchopleural Fistula (BPF) represents one of the gravest complications post-lobectomy. Present treatment strategies encompass a wide array of surgical techniques complemented by essential adjunct therapies. Despite numerous treatment modalities, mortality rates associated with BPF remain disconcertingly high.

Volumetric Patch-Based Super-Resolution Reconstruction of Hyperpolarized C Cardiac MRI.

The achievable spatial resolution of C metabolic images acquired with hyperpolarized C-pyruvate is worse than H images typically by an order of magnitude due to the rapidly decaying hyperpolarized signals and the low gyromagnetic ratio of C. This study is to develop and characterize a volumetric patch-based super-resolution reconstruction algorithm that enhances spatial resolution C cardiac MRI by utilizing structural information from H MRI. The reconstruction procedure comprises anatomical segmentation from high-resolution H MRI, calculation of a patch-based weight matrix, and iterative reconstruction of high-resolution multi-slice C MRI.

Influence of inhaled nitric oxide on bronchopulmonary dysplasia in preterm infants with PPHN or HRF at birth: a propensity score matched study.

Background: Bronchopulmonary Dysplasia (BPD) is a chronic lung disease affecting preterm infants, with limited prevention and treatment options. Inhaled Nitric Oxide (iNO) is sometimes used to treat Persistent Pulmonary Hypertension of the Newborn (PPHN) and Hypoxemic Respiratory Failure (HRF), and its impact on BPD development remains debated.

Objective: To assess whether iNO-related factors are potential contributors to the development of BPD Grade Ⅱ-Ⅲ in very premature infants (VPI) diagnosed with PPHN or HRF at birth using Propensity Score Matching (PSM).

Brg1 and RUNX1 synergy in regulating TRPM4 channel in mouse cardiomyocytes.

Background: Transient Receptor Potential Melastatin 4 (TRPM4), a non-selective cation channel, plays a critical role in cardiac conduction abnormalities. Brg1, an ATP-dependent chromatin remodeler, is essential for regulating gene expression in both heart development and disease. Our previous studies demonstrated Brg1 impacted on cardiac sodium/potassium channels and electrophysiological stability, its influence on TRPM4 expression and function remained unexplored.

Macranthoidin B restrains the epithelial-mesenchymal transition through COX-2/PGE pathway in endometriosis.

Introduction: Macranthoidin B is one of the primary and unique triterpenoid saponin metabolites from Hand. -Mazz, which is used to treat endometriosis (EMS) in traditional Chinese medicine. However, the effect of macranthoidin B remains unknown in EMS.

Quantitative hemodynamics of draining veins in brain arteriovenous malformation: a preliminary study based on computational fluid dynamics.

Objective: This study initiated a preliminary computational fluid dynamics (CFD)-based study to investigate the relationship between quantitative hemodynamics of arteriovenous malformation (AVM) draining veins and rupture.

Methods: The quantitative hemodynamics of AVM draining veins were generated from computed tomography angiography (CTA)-based steady-state CFD models. Morphological and hemodynamic parameters were compared between the ruptured and unruptured groups.

Ferrostatin-1 improves acute sepsis-induced cardiomyopathy via inhibiting neutrophil infiltration through impaired chemokine axis.

Introduction: Sepsis-induced cardiomyopathy is a common complication of sepsis and is associated with higher mortality. To date, effective diagnostic and management strategies are still lacking. Recent studies suggest that ferroptosis plays a critical role in sepsis-induced cardiomyopathy and ferroptosis inhibitor Ferrostatin-1 (Fer-1) improved cardiac dysfunction and survival in lipopolysaccharide (LPS) induced endotoxemia.

One-Step Image Reconstruction for Cine MRI with a Quadratic Constraint.

Motivation: In cine MRI, the measurements within each timeframe alone are too noisy for image reconstruction. Some information must be 'borrowed' from other time frames and the reconstruction algorithm is a slow iterative procedure.

Goals: We set up a constrained objective function, which uses the measurements at other time frames to regularize the image reconstruction.

SOX13 as a potential prognostic biomarker linked to immune infiltration and ferroptosis inhibits the proliferation, migration, and metastasis of thyroid cancer cells.

Background: SOX13 is a transcription factor belonging to the SOX family. SOX proteins are critical regulators of multiple cancer progression, and some are known to control carcinogenesis. Nevertheless, the functional and clinical significance of SOX13 in human thyroid cancer (THCA) remain largely unelucidated.

Immune imbalance in Lupus Nephritis: The intersection of T-Cell and ferroptosis.

Ferroptosis is a novel form of cell death characterized by unlimited accumulation of iron-dependent lipid peroxides. It is often accompanied by disease, and the relationship between ferroptosis of immune cells and immune regulation has been attracting increasing attention. Initially, it was found in cancer research that the inhibition of regulatory T cell (Treg) ferroptosis and the promotion of CD8+ T cell ferroptosis jointly promoted the formation of an immune-tolerant environment in tumors.

Metabolic crosstalk between roots and rhizosphere drives alfalfa decline under continuous cropping.

Considerable biological decline of continuously cropped alfalfa may be tightly linked to rhizosphere metabolism. However, plant-soil feedbacks and age-related metabolic changes in alfalfa stands remain unexplored. The aim of this study was to identify the linkages of rhizosphere and root metabolites, particularly autotoxins and prebiotics, to alfalfa decline under continuous cropping.

Haemoglobin for Fall Risk Screening in Gynaecological and Obstetric Wards: Retrospective Survey and Delphi Validation.

Aims: The objective of this study is to ascertain the suitability of haemoglobin as a screening factor for falls among obstetrics and gynaecology inpatients and to formulate a stratified scheme for assessing fall risk based on haemoglobin.

Design: A retrospective analysis and Delphi surveys were employed for this investigation.

Methods: Initially, a retrospective survey analysed falls among obstetrics and gynaecology inpatients in two hospitals from January 1, 2020, to July 10, 2022.

Total Syntheses of Diepoxy-Kaurane Diterpenoids Enabled by a Bridgehead-Enone-Initiated Intramolecular Cycloaddition.

Here, we report the enantioselective total syntheses of four diepoxy--kaurane diterpenoids including (-)-Macrocalin B, (-)-Acetyl-macrocalin B, and (-)-Isoadenolin A and the revised structure of (-)-Phyllostacin I, which hinges on the strategic design of a regioselective and stereospecific trapping of a highly reactive [3.2.1]-bridgehead enone intermediate via a tethered intramolecular Diels-Alder reaction.

Evaluating PAX1/JAM3 methylation for triage in HPV 16/18-infected women.

Objective: Referring all women who tested positive for human papillomavirus (HPV) 16/18 to colposcopy may lead to potential over-referral issues. Triage tests based on cytology results face challenges in achieving accurate diagnoses. Our study aims to assess the clinical effectiveness of PAX1/JAM3 methylation (CISCER) test as a triage method for HPV 16/18-positive women.

GsMYB10 encoding a MYB-CC transcription factor enhances the tolerance to acidic aluminum stress in soybean.

Background: MYB transcription factors (TFs) play crucial roles in the response to diverse abiotic and biotic stress factors in plants. In this study, the GsMYB10 gene encoding a MYB-CC transcription factor was cloned from wild soybean BW69 line. However, there is less report on the aluminum (Al)-tolerant gene in this subfamily.

Investigating the inflammatory mechanism of notoginsenoside R1 in Diabetic nephropathy via ITGB8 based on network pharmacology and experimental validation.

Background: Diabetes often causes diabetic nephropathy (DN), a serious long-term complication. It is characterized by chronic proteinuria, hypertension, and kidney function decline, can progress to end-stage renal disease, lowering patients' quality of life and lifespan. Inflammation and apoptosis are key to DN development.

Detection of a novel DNA methylation marker panel for esophageal cancer diagnosis using circulating tumor DNA.

Background: Esophageal cancer (ECa) is one of the most deadly cancers, with increasing incidence worldwide and poor prognosis. While endoscopy is recommended for the detection of ECa in high-risk individuals, it is not suitable for large-scale screening due to its invasiveness and inconvenience.

Methods: In this study, a novel gene methylation panel was developed for a blood-based test, and its diagnostic efficacy was evaluated using a cohort of 304 participants (203 cases, 101 controls).

Secreted Xylanase PstXyn1 Contributes to Stripe Rust Infection Possibly by Overcoming Cell Wall Barrier and Suppressing Defense Responses in Wheat.

f. sp. () secretes a plethora of cell wall-degrading enzymes (CWDEs) to facilitate fungal invasion during infection.