Determination of N-acetyl-β-hexosaminidase in serum from hemolyzed blood.

Background: Determination of lysosomal N-acetyl-β-hexosaminidase (HEX) in serum from hemolyzed blood, creates serious analytical problems, because hemoglobin absorbs light at a similar wavelength like 4-nitrophenol, which is released from artificial substrate.

Objective: The objective of the work was to adapt a manual method to allow analysis of HEX in hemolyzed samples.

Methods: Serums without and with hemolysis were incubated with 4-nitrophenol-N-acetylglucosamine as a substrate.