Development and Validation of High Performance Liquid Chromatographic Method for Determination of Gentisic Acid and Related Renal Cell Carcinoma Biomarkers in Urine.

A reversed phase liquid chromatographic (RPLC) method was developed to simultaneously detect and quantify creatinine, quinolinic acid, gentisic acid and 4-hydroxybenzoic acid in urine. These four bio-markers are present in relatively high concentrations in urine. Using a 5% methanol in water mobile phase with 0.