The incorporation of 3H-thymidine and 3H-deoxycytidine into acidoprecipitable fraction of hamster cells transformed by herpes simplex viruses type 1 and type 2 and of 3H-thymidine into hamster cells transformed by human cytomegalovirus was found to be resistant to the action of cytosine arabinoside. More 3H-thymidine was incorporated into these cells in the presence than in the absence of the drug. Similar stimulaton of 3H-thymidine uptake could be achieved by using unlabelled deoxycytidine instead of cytosine arabinoside.
View Article and Find Full Text PDFHerpes simplex virus type 1, strain Kupka, did not replicate in chick embryo fibroblasts (CEF), but the infection was followed by the development of cytopathic changes. This effect could be abolished by UV irradiation of the virus. Virus-induced thymidine kinase was synthesized in the infected cells reaching a maximum level at 24 hours post infection (p.
View Article and Find Full Text PDFIn human diploid fibroblast LEP cells infected with AD169 strain of human cytomegalovirus (CMV) a sharp increase of cytosol thymidine kinase activity was observed. The properties of the cytosol enzymes from infected and non-infected cells were compared. No significant differences between the enzymes from infected and control cells were observed in substrate specificity, pH dependence, thermostability and relative electrophoretic mobility.
View Article and Find Full Text PDFThe gal-bio region was transduced from Escherichia coli B to K12 and lysogenized with lambda b2. By means of P1 transduction mapping, the lambda b2 prophage was shown to be inserted between gal and bio and to be circularly permuted in the same way as lambda b2+ in K12. Increased frequency of recombinations within the prophage was observed when the viral genes were mapped with respect to bio instead of to gal.
View Article and Find Full Text PDFFolia Microbiol (Praha)
October 1968
Folia Microbiol (Praha)
November 1965
Biochem Biophys Res Commun
June 1965