Transcriptional regulation of the human trefoil factor, TFF1, by gastrin.

Background & Aims: This study aimed to identify gastrin-sensitive genes that may mediate the effects of this hormone on gastric epithelial architecture.

Methods: Gastrin-sensitive genes were identified by messenger RNA (mRNA) differential display of the gastric fundus from gastrin-deficient (GAS-KO) or wild-type mice. Gastrin-stimulated expression of the trefoil peptide TFF1 in mouse fundus and in the gastric cancer cell line AGS-G(R) was determined by Northern blot and real-time polymerase chain reaction.