Development and validation of a survival prediction model for patients with advanced non-small cell lung cancer based on LASSO regression.

Unlabelled: Lung cancer remains a significant global health burden, with non-small cell lung cancer (NSCLC) being the predominant subtype. Despite advancements in treatment, the prognosis for patients with advanced NSCLC remains unsatisfactory, underscoring the imperative for precise prognostic assessment models. This study aimed to develop and validate a survival prediction model specifically tailored for patients diagnosed with NSCLC.

Association between systemic lupus erythematosus and common female reproductive system malignancies.

Lymphocytes are important for protective immunity against infections and cancers, and dysregulation of the immune system may lead to systemic lupus erythematosus (SLE). Metabolic adaptation regulates the fate of lymphocytes. The immune microenvironment is vital role in both SLE and gynecological malignancies.

Prognostic value of postoperative radiotherapy in patients with vulvar squamous carcinoma: findings based on the SEER database.

Introduction: The role of postoperative radiotherapy in treating squamous cell carcinoma of the vulva remains controversial. This study evaluated the effect of radiotherapy on the survival of patients with postoperative squamous cell carcinoma of the vulva.

Methods: Clinical and prognostic information on patients diagnosed with vulvar squamous cell carcinoma from 2010 to 2015 was collected from the Surveillance, Epidemiology, and Prognosis (SEER) database.

Blockade of autophagy aggravates endoplasmic reticulum stress and improves Paclitaxel cytotoxicity in human cervical cancer cells.

Purpose: Autophagy is one of the ways to degrade unfolded proteins after endoplasmic reticulum (ER) stress. The purpose of this study is to determine whether a blockade of autophagy leads to aggravated endoplasmic reticulum stress, which then induces cells apoptosis in HeLa cells treated with paclitaxel.

Materials And Methods: Autophagy activation and the proapoptotic effects were characterized using monodansylcadaverine labeling and Hoechest staining, respectively.

Expression of Derlin-1 and its effect on expression of autophagy marker genes under endoplasmic reticulum stress in lung cancer cells.

Background: Recent findings indicated that Derlin-1 has an important function in tumour progression. In this study, we aimed to determine whether Derlin-1 has an oncogene function as a cross-talk molecule with autophagy.

Methods: Cancer cells were treated with tunicamycin (TM) for 8 and 24 h.

Inhibition of autophagy-potentiated chemosensitivity to cisplatin in laryngeal cancer Hep-2 cells.

Objective: The purposes of this study were to determine whether autophagy was involved in cisplatin (CDDP) resistance and to investigate the role of the autophagy in the regulation of chemosensitivity to CDDP in laryngeal cancer Hep-2 cells.

Methods: A WST-1 assay was performed to determine cell viability and cell proliferation. Autophagy activation and proapoptotic effects were characterized using monodansylcadaverine labeling and Hoechest staining, respectively.

[Effect of autophagy gene Beclin 1 on the growth of cervical cancer HeLa cells in vitro and vivo].

Objective: To investigate the effects of autophagy gene Beclin 1 on growth of cervical cancer HeLa cells in vitro and vivo.

Methods: The eukaryotic expression vector of Beclin1 was constructed and transfected via lipofectamine into HeLa cells. The experimental cells were classified into 3 groups: pcDNA3.

Clinicopathologic correlations between human papillomavirus 16 infection and Beclin 1 expression in human cervical cancer.

Our earlier study showed that the autophagy gene Beclin 1 could affect cell proliferation in a cervical cancer HeLa cell line. In this study, we examined Beclin 1 protein expression in 81 specimens of cervical squamous carcinoma by immunohistochemistry. Meanwhile, we detected E6 and E7 genes of human papillomavirus 16 in these tissues by polymerase chain reaction.

[Effect and mechanism of an autophagy gene beclin 1 on cervical cancer HeLa cells].

Objective: To investigate the inhibitory effects and the mechanism of autophagy gene beclin 1 on cervical cancer HeLa cells.

Methods: The eukaryotic expression vector and short hairpin RNA (shRNA) expression vector of beclin 1 were transfected via lipofectamine into HeLa cells. Experimental cells were classified into 5 groups: pcDNA3.

Combination of IL-24 and cisplatin inhibits cervical cancer growth in a xenograft nude mice model.

IL-24/mda-7 delivery augments the sensitivity of several tumor types to cisplatin but the underlying mechanism(s) are unclear. Here, we used a cervical cancer xenograft model in nude mice to further elucidate the interaction between IL-24 and cisplatin. Nude mice were inoculated subcutaneously in the left axilla with Hela cells and randomly grouped into 5 treatment schedules: PBS (I); pDC316 vector (II); pDC316-hIL-24 (III); cisplatin (IV); and pDC316-hIL-24 combined with cisplatin (V).

[Size distribution of particles collected by passive sampler].

Airborn particulate matters were sampled by two duplicate glass slides coated with thin layer of vaseline laid at the bottom of sampling cylinder of passive sampler for collecting both gaseous and particulate phase semi-volatile organic pollutant. Their size distribution was analyzed and found to be influenced by wind speed and coverage of the fine-screen-mesh (300 mesh) wrapping around the outside of sampling cylinder. In a windless (indoor) and semi-static windy conditions (sealed courtyard), diffusion of coarse particles (> 50 microm) was reduced effectively by covering sampling cylinder with the fine-screen-mesh, and accumulated size distribution curve of PM10 was similar with those collected by an active size-fractionated sampler (cascade impactor).

[Growth inhibitory effects of Beclin1 gene on epithelial ovarian cancer SKOV3 cells].

Objective: To explore the effect of Beclin1 overexpression on the growth of ovarian carcinoma cell line SKOV3 in vitro and in vivo.

Methods: The recombinant plasmid pcDNA3.1/Beclin1 was constructed and transfected into SKOV3 cells via lipofectamine 2000.

A passive sampler with improved performance for collecting gaseous and particulate phase polycyclic aromatic hydrocarbons in air.

A passive sampler which can be used to collect both gaseous and particulate phase polycyclic aromatic hydrocarbons (PAHs) in air was previously developed and calibrated. The sampler was successfully used in a field study in North China Plain. However, the relatively low uptake rate for particulate phase PAHs prevented it from being applied in nonseverely contaminated environment.

Morphology of single inhalable particle in the air polluted city of Shijiazhuang, China.

In the typical air polluted city of Shijiazhuang, single inhalable particle samples in non-heating period, heating period, dust storm days, and snowy days were collected and detected by SEM/EDS (scanning electron microscopy and energy dispersive X-ray spectrometry). The particle morphology was characterized by the 6 shape clusters, which are: irregular square, agglomerate, sphere, floccule, column or stick, and unknown, by quantitative order. The irregular square particles are common in all kinds of samples; sphere particles are more, and column or stick are less in winter samples; in the wet deposit samples, agglomerate and floccule particles are not found.

[Size distribution and shape characteristics of subaerial airborne particles].

This research sampled airborne particles in the typical air polluted city of Shijiazhuang to measure the particle size and shape by the instrument of the CIS-50 and the scan electronic microscope in the non-heating period and heating period. The results show that the subaerial airborne particle size distribution mode is coarse with the size range of 0.8-120 microm, mostly under 10 microm, and the semi-square & square like particle shape is dominant, the sphere like lesser, the acute-angle and lathy like sparse.

Beclin 1-mediated macroautophagy involves regulation of caspase-9 expression in cervical cancer HeLa cells.

Objective: To investigate the role of Beclin 1 in HeLa cells and to obtain further insight into the relationship between autophagy and apoptosis.

Methods: Beclin 1 silencing was achieved using RNA interference. The expression of gene was measured using quantitative real time RT-PCR and Western blotting.

[Construction of shRNA expression vectors for autophagy gene beclin 1 and their downregulation effect on caspase-9].

The shRNA expression vectors were constructed and transfected via lipofectamine into HeLa cells. Real time-ploymerase chain reaction (RT-PCR) and Western blot were used for detecting the expression of mRNA and protein of Beclin1 in transfected cells. Flow cytometry was employed to observe the effect of transfection on the apoptosis and cell cycle of HeLa, and proliferation was analyzed by MTT assay.

[Expression and involved signal transduction pathway of autophagy gene Beclin 1 in epithelial ovarian cancer].

Objective: To investigate the relationship between the autophagy gene Beclin 1 involving the PI3K/PKB signaling pathway and the occurrence, development of epithelial ovarian carcinoma.

Methods: The expression of Beclin 1, Class I PI3K (p110alpha), Class III PI3K (hvps34) or p-PKB was, by immunohistochemistry, detected respectively in 25 normal ovarian tissues, 25 benign neoplasia tissues, 19 borderline tissues, and 69 epithelial ovarian carcinoma tissues.

Results: The higher expressions of Beclin 1 and hvps34 were found in normal and benign ovarian neoplasia tissues; the expressions were reduced in the borderline lesion tissue; and the lowest level of expressions could be detected in the ovarian carcinoma tissue (P < 0.

[Construction of shRNA expression vectors for autophagy gene Beclin 1 and effect of the vectors on transfected HeLa cells in vitro].

Objective: Autophagy gene Beclin 1 plays an important role in several types of human cancer. In this study, RNA interference (RNAi) technique was employed to determine the effect of inhibiting Beclin 1 on the growth of tumor cells.

Methods: According to the encoding sequence of mRNA of Beclin 1, the target site for the RNAi technique was designed and the vector for shRNA (short hairpin RNA) expression in tumor cells was constructed.

[Correlation of autophagy gene Beclin1 to tumorigenesis and development of epithelial ovarian cancer].

Background & Objective: Some studies have showed that autophagy suppression may result in malignant transformation, and the inactivation of autophagy gene Beclin1 induces malignancy. This study was to investigate the role of Beclin1 in the tumorigenesis and development of epithelial ovarian carcinoma, and to explore the effect of Beclin1 overexpression on the growth of ovarian carcinoma cell line SKOV3 in vitro.

Methods: The expression of Beclin1 in 25 specimens of normal ovarian tissue, 25 specimens of benign ovarian neoplasia, 19 specimens of borderline ovarian tissue, and 69 specimens of epithelial ovarian carcinoma was detected by immunohistochemistry.

[Expression and clinical significance of autophagy gene Beclin 1 in cervical squamous cell carcinoma].

Objective: Autophagy gene Beclin 1 plays an important role in several types of human cancers. So we, in this study, employed the immunohistochemical manner to detect the Beclin 1 gene expression and explore its clinical significance in cervical aquamous cell carcinoma.

Methods: SP immunohistochemistry technique was used to detect the expression of Beclin 1 gene in specimens of 81 cervical squamous cell carcinoma, 20 cervical intraepithelial neoplasm (CIN, II - III), and 20 normal cervix.