Publications by authors named "Zang Xiao-Nan"
Article Synopsis
- Natural astaxanthin is a potent antioxidant derived from certain organisms, and the study focuses on how lipids impact its accumulation through specific gene expressions.
- Co-expressing genes involved in astaxanthin synthesis led to an increase in its precursor (zeaxanthin) and altered fatty acid compositions, resulting in higher astaxanthin levels in the transformed strains by nearly 50%.
- The research highlights the connection between lipid metabolism and astaxanthin production, suggesting that enhancing fatty acid synthesis could effectively boost astaxanthin yields.
Article Synopsis
- Phycocyanin and allophycocyanin are valuable due to their fluorescence and antioxidant properties, prompting researchers to create recombinant strains to enhance production and enable easier modification.
- The study involved constructing seven different recombinant strains, which included individual proteins and combinations, revealing variations in molecular weights and confirmation of dimer and polymer formations through mass spectrometry.
- Fluorescence analysis showed that recombinant phycocyanin has a peak around 640 nm, exhibiting higher intensity compared to allophycocyanin, indicating its potential use as a fluorescence probe in medical applications.
has attracted wide attention as a cyanobacteria with high nutritional value. In this research, the response surface method was used to study the effects of light cycle, light intensity and red-blue LED conditions on the growth and organic matter accumulation in spiral shaped strain OUC623 and linear shaped strain OUC793. The light conditions suitable for OUC623 were as follows: growth (light time 12.
View Article and Find Full Text PDFTo study the assembly of phycocyanin β subunit, the gene cpcT was first cloned from Arthrospira platensis FACHB314. To explore the function of cpcT, the DNA of phycocyanin β subunit and cpcT were transformed into Escherichia coli BL21 with the plasmid pET-hox1-pcyA, which contained the genes hemeoxygenase 1 (Hox1) and ferredoxin oxidoreductase (PcyA) needed to produce phycocyanobilin. The transformed strains showed specific phycocyanin fluorescence, and the fluorescence intensity was stronger than the strains with only phycocyanin β subunit, indicating that CpcT can promote the assembly of phycocyanin to generate fluorescence.
View Article and Find Full Text PDFTo study the response of Gracilaria lemaneiformis to heat stress, two key enzymes - ubiquitin-activating enzyme (E1) and ubiquitin-conjugating enzyme (E2) - of the Ubiquitin/26S proteasome pathway (UPP) were studied in three strains of G. lemaneiformis-wild type, heat-tolerant cultivar 981 and heat-tolerant cultivar 07-2. The full length DNA sequence of E1 contained only one exon.
View Article and Find Full Text PDFHigh manufacturing costs and oral delivery are the constraints in clinical application of calcitonin. We selected surface-displayed Saccharomyces cerevisiae as a low-cost and safe carrier for oral delivery of salmon calcitonin (sCT). The sCT DNA fragment, optimized according to the codon preference of S.
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